A high performance thin layer chromatographic method was developed for the simultaneous estimation of Tizanidine and Diclofenac in tablet formulations. The separation was achieved by precoated Silicagel F254 and the solvent system used for the separation was chloroform and methanol (80:20 v/v) as eluent. The detection was carried out at 230 nm. Cetirizine was used as an internal standard. The solvent system was found to give compact spots for diclofenac sodium (Rf value of 0.86±0.01), tizanidine (0.26±0.01) and cetirizine (0.52±0.02). The method has been validated for linearity accuracy and precision. Linearity for tizanidine and diclofenac sodium were in the range of 0.6-1.4 μg/ml and 7.5-17.5 μg/ml, respectively. The mean recoveries obtained for tizanidine and diclofenac sodium were 98.73% and 99.70%, respectively. The proposed method was accurate, precise, selective and rapid for simultaneous estimation of tizanidine and diclofenac sodium in tablets.
|Number of pages||4|
|Publication status||Published - 2005|