HSV susceptibility to acyclovir – genotypic and phenotypic characterization

Raksha Vikas, Suresha G. Prabhu, Piya P. Mudgal, Ujwal Shetty, Kavitha Karunakaran, Anitha Jagadesh, Amogh Auti, Rithu P. Stansilaus, Sudheesh Nair, Govindakarnavar Arunkumar

Research output: Contribution to journalArticle

Abstract

Background: Infections due to drug-resistant herpes simplex viruses (HSV) represent an important clinical concern, especially in immunocompromised patients. The present study was aimed at detecting acyclovir (ACV) susceptibility in HSV clinical samples. Methods: A total of 13 HSV-positive clinical samples (5 HSV-1 and 8 HSV-2) recovered from patients (1 immunocompromised and 12 of unknown immune status) were included in the study. The genotypic analysis involved an initial UL23 (thymidine kinase) gene sequencing, followed by a confirmatory phenotypic assay using plaque reduction technique. Results: Two novel amino acid changes, A37V and H283N, were detected in HSV-1 positive clinical samples, which were found to be susceptible to acyclovir (half maximal effective concentration = 1.5 µM) by plaque reduction assay. Conclusions: These two novel amino acid changes could be therefore considered as natural polymorphisms, a phenomenon widely associated with the HSV-UL23 gene.

Original languageEnglish
Pages (from-to)141-145
Number of pages5
JournalAntiviral Therapy
Volume24
Issue number2
DOIs
Publication statusPublished - 01-01-2019

All Science Journal Classification (ASJC) codes

  • Pharmacology
  • Pharmacology (medical)
  • Infectious Diseases

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  • Cite this

    Vikas, R., Prabhu, S. G., Mudgal, P. P., Shetty, U., Karunakaran, K., Jagadesh, A., Auti, A., Stansilaus, R. P., Nair, S., & Arunkumar, G. (2019). HSV susceptibility to acyclovir – genotypic and phenotypic characterization. Antiviral Therapy, 24(2), 141-145. https://doi.org/10.3851/IMP3279