HSV susceptibility to acyclovir – genotypic and phenotypic characterization

Raksha Vikas, Suresha G. Prabhu, Piya P. Mudgal, Ujwal Shetty, Kavitha Karunakaran, Anitha Jagadesh, Amogh Auti, Rithu P. Stansilaus, Sudheesh Nair, Govindakarnavar Arunkumar

Research output: Contribution to journalArticle

Abstract

Background: Infections due to drug-resistant herpes simplex viruses (HSV) represent an important clinical concern, especially in immunocompromised patients. The present study was aimed at detecting acyclovir (ACV) susceptibility in HSV clinical samples. Methods: A total of 13 HSV-positive clinical samples (5 HSV-1 and 8 HSV-2) recovered from patients (1 immunocompromised and 12 of unknown immune status) were included in the study. The genotypic analysis involved an initial UL23 (thymidine kinase) gene sequencing, followed by a confirmatory phenotypic assay using plaque reduction technique. Results: Two novel amino acid changes, A37V and H283N, were detected in HSV-1 positive clinical samples, which were found to be susceptible to acyclovir (half maximal effective concentration = 1.5 µM) by plaque reduction assay. Conclusions: These two novel amino acid changes could be therefore considered as natural polymorphisms, a phenomenon widely associated with the HSV-UL23 gene.

Original languageEnglish
Pages (from-to)141-145
Number of pages5
JournalAntiviral Therapy
Volume24
Issue number2
DOIs
Publication statusPublished - 01-01-2019

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Acyclovir
Simplexvirus
Human Herpesvirus 1
Immunocompromised Host
Amino Acids
Human Herpesvirus 2
Thymidine Kinase
Genes
Infection
Pharmaceutical Preparations

All Science Journal Classification (ASJC) codes

  • Pharmacology
  • Pharmacology (medical)
  • Infectious Diseases

Cite this

Vikas, Raksha ; Prabhu, Suresha G. ; Mudgal, Piya P. ; Shetty, Ujwal ; Karunakaran, Kavitha ; Jagadesh, Anitha ; Auti, Amogh ; Stansilaus, Rithu P. ; Nair, Sudheesh ; Arunkumar, Govindakarnavar. / HSV susceptibility to acyclovir – genotypic and phenotypic characterization. In: Antiviral Therapy. 2019 ; Vol. 24, No. 2. pp. 141-145.
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Vikas, R, Prabhu, SG, Mudgal, PP, Shetty, U, Karunakaran, K, Jagadesh, A, Auti, A, Stansilaus, RP, Nair, S & Arunkumar, G 2019, 'HSV susceptibility to acyclovir – genotypic and phenotypic characterization', Antiviral Therapy, vol. 24, no. 2, pp. 141-145. https://doi.org/10.3851/IMP3279

HSV susceptibility to acyclovir – genotypic and phenotypic characterization. / Vikas, Raksha; Prabhu, Suresha G.; Mudgal, Piya P.; Shetty, Ujwal; Karunakaran, Kavitha; Jagadesh, Anitha; Auti, Amogh; Stansilaus, Rithu P.; Nair, Sudheesh; Arunkumar, Govindakarnavar.

In: Antiviral Therapy, Vol. 24, No. 2, 01.01.2019, p. 141-145.

Research output: Contribution to journalArticle

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Vikas R, Prabhu SG, Mudgal PP, Shetty U, Karunakaran K, Jagadesh A et al. HSV susceptibility to acyclovir – genotypic and phenotypic characterization. Antiviral Therapy. 2019 Jan 1;24(2):141-145. https://doi.org/10.3851/IMP3279