Hydrolysis of rice bran oil using an immobilized lipase from Candida rugosa in isooctane

V. Ramachandra Murty, Jayadev Bhat, P. K.A. Muniswaran

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

The kinetics of enzymatic hydrolysis of rice bran oil in isooctane by immobilized Candida rugosa lipase in a batch reactor showed competitive inhibition by isooctane with a dissociation constant, KI, of 0.92 M. Continuous hydrolysis of rice bran oil was performed in recycling, packed bed reactor with 4352 U of immobilized lipase; the optimum recycle ratio was 9 and the operational half-life was 360 h without isooctane but 288 h with 25% (v/v) isooctane in rice bran oil.

Original languageEnglish
Pages (from-to)563-567
Number of pages5
JournalBiotechnology Letters
Volume26
Issue number7
DOIs
Publication statusPublished - 01-04-2004

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Candida
Lipases
Lipase
Hydrolysis
Enzyme inhibition
Enzyme kinetics
Enzymatic hydrolysis
Batch reactors
Packed beds
Recycling
Half-Life
Oils
rice bran oil
2,2,4-trimethylpentane

All Science Journal Classification (ASJC) codes

  • Biotechnology

Cite this

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Hydrolysis of rice bran oil using an immobilized lipase from Candida rugosa in isooctane. / Murty, V. Ramachandra; Bhat, Jayadev; Muniswaran, P. K.A.

In: Biotechnology Letters, Vol. 26, No. 7, 01.04.2004, p. 563-567.

Research output: Contribution to journalArticle

TY - JOUR

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