Hydrolysis of rice bran oil using an immobilized lipase from Candida rugosa in isooctane

V. Ramachandra Murty, Jayadev Bhat, P. K.A. Muniswaran

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The kinetics of enzymatic hydrolysis of rice bran oil in isooctane by immobilized Candida rugosa lipase in a batch reactor showed competitive inhibition by isooctane with a dissociation constant, KI, of 0.92 M. Continuous hydrolysis of rice bran oil was performed in recycling, packed bed reactor with 4352 U of immobilized lipase; the optimum recycle ratio was 9 and the operational half-life was 360 h without isooctane but 288 h with 25% (v/v) isooctane in rice bran oil.

Original languageEnglish
Pages (from-to)563-567
Number of pages5
JournalBiotechnology Letters
Issue number7
Publication statusPublished - 01-04-2004


All Science Journal Classification (ASJC) codes

  • Biotechnology

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