Hydrolysis of rice bran oil using immobilized lipase in a stirred batch reactor

V. Ramachandra Murty, Jayadev Bhat, P. K.A. Muniswaran

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Candida cylindracea lipase was immobilized by adsorption on acid washed glass beads. It was observed that protein loading of the support depends on the size of the particle, with smaller particle containing higher amount of protein per unit weight. Initial reaction rate linearly varied up to enzyme concentration of 17.25 U/mL. Amount of free fatty acids produced was linearly proportional up to the enzyme loading of 1650 μg/g of bead. Achievement of chemical equilibrium took longer time in the case of less protein loading. Degree of hydrolysis was found to decrease in second and third consecutive batch operations on repeated use of immobilized lipase.

Original languageEnglish
Pages (from-to)367-370
Number of pages4
JournalBiotechnology and Bioprocess Engineering
Volume7
Issue number6
DOIs
Publication statusPublished - 01-01-2002

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Lipases
Batch reactors
Lipase
Hydrolysis
Proteins
Enzymes
Candida
Fatty acids
Particle Size
Nonesterified Fatty Acids
Adsorption
Reaction rates
Glass
Weights and Measures
Acids
Oils
rice bran oil

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology
  • Biomedical Engineering

Cite this

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Hydrolysis of rice bran oil using immobilized lipase in a stirred batch reactor. / Murty, V. Ramachandra; Bhat, Jayadev; Muniswaran, P. K.A.

In: Biotechnology and Bioprocess Engineering, Vol. 7, No. 6, 01.01.2002, p. 367-370.

Research output: Contribution to journalArticle

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