Identification and characterization of phosphoseryl-tRNA[Ser]Sec kinase

Bradley A. Carlson, Xue Ming Xu, Gregory V. Kryukov, Mahadev Rao, Marla J. Berry, Vadim N. Gladyshev, Dolph L. Hatfield

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Abstract

In 1970, a kinase activity that phosphorylated a minor species of seryl-tRNA to form phosphoseryl-tRNA was found in rooster liver [Maenpaa, P. H. & Bernfield, M. R. (1970) Proc. Natl. Acad. Sci. USA 67, 688-695], and a minor seryl-tRNA that decoded the nonsense UGA was detected in bovine liver. The phosphoseryl-tRNA and the minor UGA-decoding seryl-tRNA were subsequently identified as selenocysteine (Sec) tRNA[ser]Sec, but the kinase activity remained elusive. Herein, by using a comparative genomics approach that searched completely sequenced archaeal genomes for a kinase-like protein with a pattern of occurrence similar to that of components of Sec insertion machinery, we detected a candidate gene for mammalian phosphoseryl-tRNA[Ser]Sec kinase (pstk). Mouse pstk was cloned, and the gene product (PSTK) was expressed and characterized. PSTK specifically phosphorylated the seryl moiety on seryl-tRNA[Ser]Sec and, in addition, had a requirement for ATP and Mg2+. Proteins with homology to mammalian PSTK occur in Drosophila, Caenorhabditis elegans, Methanopyrus kandleri, and Methanococcus jannaschii, suggesting a conservation of its function across archaea and eukaryotes that synthesize selenoproteins and the absence of this function in bacteria, plants, and yeast. The fact that PSTK has been highly conserved in evolution suggests that it plays an important role in selenoprotein biosynthesis and/or regulation.

Original languageEnglish
Pages (from-to)12848-12853
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume101
Issue number35
DOIs
Publication statusPublished - 31-08-2004

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Transfer RNA
Selenoproteins
Selenocysteine
Archaeal Genome
Phosphotransferases
Methanocaldococcus
Liver
Archaea
Caenorhabditis elegans
Genomics
Eukaryota
Protein Kinases
Genes
Drosophila
seryl-tRNA-ATP phosphotransferase
Adenosine Triphosphate
Yeasts
Bacteria
Proteins

All Science Journal Classification (ASJC) codes

  • General

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Carlson, Bradley A. ; Xu, Xue Ming ; Kryukov, Gregory V. ; Rao, Mahadev ; Berry, Marla J. ; Gladyshev, Vadim N. ; Hatfield, Dolph L. / Identification and characterization of phosphoseryl-tRNA[Ser]Sec kinase. In: Proceedings of the National Academy of Sciences of the United States of America. 2004 ; Vol. 101, No. 35. pp. 12848-12853.
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Identification and characterization of phosphoseryl-tRNA[Ser]Sec kinase. / Carlson, Bradley A.; Xu, Xue Ming; Kryukov, Gregory V.; Rao, Mahadev; Berry, Marla J.; Gladyshev, Vadim N.; Hatfield, Dolph L.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, No. 35, 31.08.2004, p. 12848-12853.

Research output: Contribution to journalArticle

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T1 - Identification and characterization of phosphoseryl-tRNA[Ser]Sec kinase

AU - Carlson, Bradley A.

AU - Xu, Xue Ming

AU - Kryukov, Gregory V.

AU - Rao, Mahadev

AU - Berry, Marla J.

AU - Gladyshev, Vadim N.

AU - Hatfield, Dolph L.

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