IM-133N modulates cytokine secretion by RAW264.7 and THP-1 cells

R. Sandeep Varma, Kanive P. Guruprasad, Kapaettu Satyamoorthy, L. M Sharath Kumar, U. Venkanna Babu, S. Pralhad Patki

    Research output: Contribution to journalArticle

    3 Citations (Scopus)

    Abstract

    An indigenous herbal extract IM-133N containing extracts of Prosopis glandulosa Torr and Symplocos racemosa Roxb were evaluated for potential immunomodulatory effects using RAW264.7 and THP-1 cells. The incubation of the cells for 24 h with IM-133N over a dose range 0-125 μg/ml did not cause cytotoxicity that exceeded 10%. The results indicated that non-cytotoxic doses of IM-133N effectively up-regulated iNOS, TNFα, IL-6, IL-10, IL-8 and IFNγ gene expression in both the RAW264.7 and THP-1 cells. The results also indicated IM-133N elicited dose-related increases in nitric oxide (NO) and tumor necrosis factor (TNF)-α production by RAW264.7 or THP-1 cells. These results demonstrated that IM-133N could stimulate NO and induced pro-inflammatory cytokine expression by monocytes/macrophages. As clinical studies have shown IM-133N to be an effective immunomodulator without any adverse effects, the results of the present study provide further support for the potential use of this agent as an immunostimulant or as an immunotherapy adjuvant.

    Original languageEnglish
    Pages (from-to)217-225
    Number of pages9
    JournalJournal of Immunotoxicology
    Volume13
    Issue number2
    DOIs
    Publication statusPublished - 03-03-2016

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    Nitric Oxide
    Tumor Necrosis Factor-alpha
    Cytokines
    Immunologic Adjuvants
    Macrophages
    Immunologic Factors
    Cytotoxicity
    Interleukin-8
    Gene expression
    Interleukin-10
    Interleukin-6
    Prosopis
    Cells
    Immunotherapy
    Monocytes
    Gene Expression

    All Science Journal Classification (ASJC) codes

    • Immunology
    • Toxicology

    Cite this

    Varma, R. Sandeep ; Guruprasad, Kanive P. ; Satyamoorthy, Kapaettu ; Kumar, L. M Sharath ; Babu, U. Venkanna ; Patki, S. Pralhad. / IM-133N modulates cytokine secretion by RAW264.7 and THP-1 cells. In: Journal of Immunotoxicology. 2016 ; Vol. 13, No. 2. pp. 217-225.
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    IM-133N modulates cytokine secretion by RAW264.7 and THP-1 cells. / Varma, R. Sandeep; Guruprasad, Kanive P.; Satyamoorthy, Kapaettu; Kumar, L. M Sharath; Babu, U. Venkanna; Patki, S. Pralhad.

    In: Journal of Immunotoxicology, Vol. 13, No. 2, 03.03.2016, p. 217-225.

    Research output: Contribution to journalArticle

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