Image analysis tools for evaluation of microscopic views of immunohistochemically stained specimen in medical research - A review

Keerthana Prasad, Gopalakrishna K. Prabhu

Research output: Contribution to journalReview article

63 Citations (Scopus)


The aim of this study is to review the methods being used for image analysis of microscopic views of immunohistochemically stained specimen in medical research. The solutions available range from general purpose software to commercial packages. Many studies have developed their own custom written programs based on some general purpose software available. Many groups have reported development of computer aided image analysis programs aiming at obtaining faster, simpler and cheaper solutions. Image analysis tools namely Aperio, Lucia, Metaview, Metamorph, ImageJ, Scion, Adobe Photoshop, Image Pro Plus are also used for evaluation of expressions using immunohistochemical staining. An overview of such methods used for image analysis is provided in this paper. This study concludes that there is good scope for development of freely available software for staining intensity quantification, which a medical researcher could easily use without requiring high level computer skills.

Original languageEnglish
Pages (from-to)2621-2631
Number of pages11
JournalJournal of Medical Systems
Issue number4
Publication statusPublished - 08-2012


All Science Journal Classification (ASJC) codes

  • Medicine (miscellaneous)
  • Information Systems
  • Health Informatics
  • Health Information Management

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