Imaging collagen and skeletal muscle fibers using Stokes-Polarimeter based Second Harmonic Generation Microscopy

Nirmal Mazumder, Jianjun Qiu, Chih Wei Hu, Han Ruei Tsai, Fu Jen Kao

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We analyzed the polarization states of SHG signals through four-channel photon counting based Stokes-polarimeter. The effects of linearly and circularly polarized light are characterized on molecular structure of starch granule. Different polarization parameters such as the degree of polarization (DOP), degree of linear polarization (DOLP), degree of circular polarization (DOCP), as well as anisotropy are measured from the acquired Stokes parameters. We present a polarimetric analysis of the SHG signal emitted by collagen type I and skeletal muscle fibers, in order to extract structural information on molecular order and orientation. With the appropriate combination of input laser polarization and the detection of output polarization states of SH signal will yield the absolute molecular orientations of the samples.

Original languageEnglish
Title of host publication2012 International Conference on Fiber Optics and Photonics, PHOTONICS 2012
Publication statusPublished - 2012
Event2012 International Conference on Fiber Optics and Photonics, PHOTONICS 2012 - Chennai, Tamil Nadu, India
Duration: 09-12-201212-12-2012

Conference

Conference2012 International Conference on Fiber Optics and Photonics, PHOTONICS 2012
CountryIndia
CityChennai, Tamil Nadu
Period09-12-1212-12-12

Fingerprint

Polarimeters
Harmonic generation
Collagen
Muscle
Microscopic examination
Polarization
Imaging techniques
Fibers
Circular polarization
Molecular orientation
Light polarization
Collagen Type I
Starch
Molecular structure
Anisotropy
Photons
Lasers

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials

Cite this

Mazumder, N., Qiu, J., Hu, C. W., Tsai, H. R., & Kao, F. J. (2012). Imaging collagen and skeletal muscle fibers using Stokes-Polarimeter based Second Harmonic Generation Microscopy. In 2012 International Conference on Fiber Optics and Photonics, PHOTONICS 2012 [6545671]
Mazumder, Nirmal ; Qiu, Jianjun ; Hu, Chih Wei ; Tsai, Han Ruei ; Kao, Fu Jen. / Imaging collagen and skeletal muscle fibers using Stokes-Polarimeter based Second Harmonic Generation Microscopy. 2012 International Conference on Fiber Optics and Photonics, PHOTONICS 2012. 2012.
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abstract = "We analyzed the polarization states of SHG signals through four-channel photon counting based Stokes-polarimeter. The effects of linearly and circularly polarized light are characterized on molecular structure of starch granule. Different polarization parameters such as the degree of polarization (DOP), degree of linear polarization (DOLP), degree of circular polarization (DOCP), as well as anisotropy are measured from the acquired Stokes parameters. We present a polarimetric analysis of the SHG signal emitted by collagen type I and skeletal muscle fibers, in order to extract structural information on molecular order and orientation. With the appropriate combination of input laser polarization and the detection of output polarization states of SH signal will yield the absolute molecular orientations of the samples.",
author = "Nirmal Mazumder and Jianjun Qiu and Hu, {Chih Wei} and Tsai, {Han Ruei} and Kao, {Fu Jen}",
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Mazumder, N, Qiu, J, Hu, CW, Tsai, HR & Kao, FJ 2012, Imaging collagen and skeletal muscle fibers using Stokes-Polarimeter based Second Harmonic Generation Microscopy. in 2012 International Conference on Fiber Optics and Photonics, PHOTONICS 2012., 6545671, 2012 International Conference on Fiber Optics and Photonics, PHOTONICS 2012, Chennai, Tamil Nadu, India, 09-12-12.

Imaging collagen and skeletal muscle fibers using Stokes-Polarimeter based Second Harmonic Generation Microscopy. / Mazumder, Nirmal; Qiu, Jianjun; Hu, Chih Wei; Tsai, Han Ruei; Kao, Fu Jen.

2012 International Conference on Fiber Optics and Photonics, PHOTONICS 2012. 2012. 6545671.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

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T1 - Imaging collagen and skeletal muscle fibers using Stokes-Polarimeter based Second Harmonic Generation Microscopy

AU - Mazumder, Nirmal

AU - Qiu, Jianjun

AU - Hu, Chih Wei

AU - Tsai, Han Ruei

AU - Kao, Fu Jen

PY - 2012

Y1 - 2012

N2 - We analyzed the polarization states of SHG signals through four-channel photon counting based Stokes-polarimeter. The effects of linearly and circularly polarized light are characterized on molecular structure of starch granule. Different polarization parameters such as the degree of polarization (DOP), degree of linear polarization (DOLP), degree of circular polarization (DOCP), as well as anisotropy are measured from the acquired Stokes parameters. We present a polarimetric analysis of the SHG signal emitted by collagen type I and skeletal muscle fibers, in order to extract structural information on molecular order and orientation. With the appropriate combination of input laser polarization and the detection of output polarization states of SH signal will yield the absolute molecular orientations of the samples.

AB - We analyzed the polarization states of SHG signals through four-channel photon counting based Stokes-polarimeter. The effects of linearly and circularly polarized light are characterized on molecular structure of starch granule. Different polarization parameters such as the degree of polarization (DOP), degree of linear polarization (DOLP), degree of circular polarization (DOCP), as well as anisotropy are measured from the acquired Stokes parameters. We present a polarimetric analysis of the SHG signal emitted by collagen type I and skeletal muscle fibers, in order to extract structural information on molecular order and orientation. With the appropriate combination of input laser polarization and the detection of output polarization states of SH signal will yield the absolute molecular orientations of the samples.

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M3 - Conference contribution

SN - 9781467347181

BT - 2012 International Conference on Fiber Optics and Photonics, PHOTONICS 2012

ER -

Mazumder N, Qiu J, Hu CW, Tsai HR, Kao FJ. Imaging collagen and skeletal muscle fibers using Stokes-Polarimeter based Second Harmonic Generation Microscopy. In 2012 International Conference on Fiber Optics and Photonics, PHOTONICS 2012. 2012. 6545671