Imaging molecular structure with Stokes-polarimeter based second harmonic generation microscopy

Nirmal Mazumder, Jianjun Qiu, Chih Wei Hu, Fu Jen Kao

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

We analyzed the polarization states of second harmonic generation (SHG) signals from starch granules and type I collagen through a four-channel photon counting based Stokes-polarimeter. The 2D SHG images of samples are reconstructed using various polarization parameters, such as the degree of polarization (DOP), the degree of linear polarization (DOLP), the degree of circular polarization (DOCP), as well as the anisotropy from the acquired Stokes parameters. Furthermore, we have demonstrated that the polarization parameters are changes at different input polarizations and focusing depths.

Original languageEnglish
Title of host publicationMultiphoton Microscopy in the Biomedical Sciences XIII
Volume8588
DOIs
Publication statusPublished - 2013
EventMultiphoton Microscopy in the Biomedical Sciences XIII - San Francisco, CA, United States
Duration: 03-02-201305-02-2013

Conference

ConferenceMultiphoton Microscopy in the Biomedical Sciences XIII
CountryUnited States
CitySan Francisco, CA
Period03-02-1305-02-13

Fingerprint

Polarimeters
Anisotropy
polarimeters
Harmonic generation
Collagen Type I
Molecular Structure
Photons
Starch
Molecular structure
Microscopy
harmonic generations
Microscopic examination
molecular structure
Polarization
microscopy
Imaging techniques
polarization
starches
collagens
circular polarization

All Science Journal Classification (ASJC) codes

  • Atomic and Molecular Physics, and Optics
  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Radiology Nuclear Medicine and imaging

Cite this

Mazumder, N., Qiu, J., Hu, C. W., & Kao, F. J. (2013). Imaging molecular structure with Stokes-polarimeter based second harmonic generation microscopy. In Multiphoton Microscopy in the Biomedical Sciences XIII (Vol. 8588). [858823] https://doi.org/10.1117/12.2003894
Mazumder, Nirmal ; Qiu, Jianjun ; Hu, Chih Wei ; Kao, Fu Jen. / Imaging molecular structure with Stokes-polarimeter based second harmonic generation microscopy. Multiphoton Microscopy in the Biomedical Sciences XIII. Vol. 8588 2013.
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abstract = "We analyzed the polarization states of second harmonic generation (SHG) signals from starch granules and type I collagen through a four-channel photon counting based Stokes-polarimeter. The 2D SHG images of samples are reconstructed using various polarization parameters, such as the degree of polarization (DOP), the degree of linear polarization (DOLP), the degree of circular polarization (DOCP), as well as the anisotropy from the acquired Stokes parameters. Furthermore, we have demonstrated that the polarization parameters are changes at different input polarizations and focusing depths.",
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Mazumder, N, Qiu, J, Hu, CW & Kao, FJ 2013, Imaging molecular structure with Stokes-polarimeter based second harmonic generation microscopy. in Multiphoton Microscopy in the Biomedical Sciences XIII. vol. 8588, 858823, Multiphoton Microscopy in the Biomedical Sciences XIII, San Francisco, CA, United States, 03-02-13. https://doi.org/10.1117/12.2003894

Imaging molecular structure with Stokes-polarimeter based second harmonic generation microscopy. / Mazumder, Nirmal; Qiu, Jianjun; Hu, Chih Wei; Kao, Fu Jen.

Multiphoton Microscopy in the Biomedical Sciences XIII. Vol. 8588 2013. 858823.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

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AB - We analyzed the polarization states of second harmonic generation (SHG) signals from starch granules and type I collagen through a four-channel photon counting based Stokes-polarimeter. The 2D SHG images of samples are reconstructed using various polarization parameters, such as the degree of polarization (DOP), the degree of linear polarization (DOLP), the degree of circular polarization (DOCP), as well as the anisotropy from the acquired Stokes parameters. Furthermore, we have demonstrated that the polarization parameters are changes at different input polarizations and focusing depths.

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Mazumder N, Qiu J, Hu CW, Kao FJ. Imaging molecular structure with Stokes-polarimeter based second harmonic generation microscopy. In Multiphoton Microscopy in the Biomedical Sciences XIII. Vol. 8588. 2013. 858823 https://doi.org/10.1117/12.2003894