Imaging molecular structure with Stokes-polarimeter based second harmonic generation microscopy

Nirmal Mazumder; Jianjun Qiu; Chih Wei Hu; Fu Jen Kao

doi:10.1117/12.2003894

Imaging molecular structure with Stokes-polarimeter based second harmonic generation microscopy

Nirmal Mazumder, Jianjun Qiu, Chih Wei Hu, Fu Jen Kao

Department of Biophysics, Manipal School of Life Sciences, Manipal

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

2 Citations (Scopus)

Abstract

We analyzed the polarization states of second harmonic generation (SHG) signals from starch granules and type I collagen through a four-channel photon counting based Stokes-polarimeter. The 2D SHG images of samples are reconstructed using various polarization parameters, such as the degree of polarization (DOP), the degree of linear polarization (DOLP), the degree of circular polarization (DOCP), as well as the anisotropy from the acquired Stokes parameters. Furthermore, we have demonstrated that the polarization parameters are changes at different input polarizations and focusing depths.

Original language	English
Title of host publication	Multiphoton Microscopy in the Biomedical Sciences XIII
Volume	8588
DOIs	https://doi.org/10.1117/12.2003894
Publication status	Published - 2013
Event	Multiphoton Microscopy in the Biomedical Sciences XIII - San Francisco, CA, United States Duration: 03-02-2013 → 05-02-2013

Conference

Conference	Multiphoton Microscopy in the Biomedical Sciences XIII
Country/Territory	United States
City	San Francisco, CA
Period	03-02-13 → 05-02-13

All Science Journal Classification (ASJC) codes

Atomic and Molecular Physics, and Optics
Electronic, Optical and Magnetic Materials
Biomaterials
Radiology Nuclear Medicine and imaging

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10.1117/12.2003894

Cite this

@inproceedings{8ca17c48f7fe4d2d8fc3ddd155639edc,

title = "Imaging molecular structure with Stokes-polarimeter based second harmonic generation microscopy",

abstract = "We analyzed the polarization states of second harmonic generation (SHG) signals from starch granules and type I collagen through a four-channel photon counting based Stokes-polarimeter. The 2D SHG images of samples are reconstructed using various polarization parameters, such as the degree of polarization (DOP), the degree of linear polarization (DOLP), the degree of circular polarization (DOCP), as well as the anisotropy from the acquired Stokes parameters. Furthermore, we have demonstrated that the polarization parameters are changes at different input polarizations and focusing depths.",

author = "Nirmal Mazumder and Jianjun Qiu and Hu, {Chih Wei} and Kao, {Fu Jen}",

year = "2013",

doi = "10.1117/12.2003894",

language = "English",

isbn = "9780819493576",

volume = "8588",

booktitle = "Multiphoton Microscopy in the Biomedical Sciences XIII",

note = "Multiphoton Microscopy in the Biomedical Sciences XIII ; Conference date: 03-02-2013 Through 05-02-2013",

}

TY - GEN

T1 - Imaging molecular structure with Stokes-polarimeter based second harmonic generation microscopy

AU - Mazumder, Nirmal

AU - Qiu, Jianjun

AU - Hu, Chih Wei

AU - Kao, Fu Jen

PY - 2013

Y1 - 2013

N2 - We analyzed the polarization states of second harmonic generation (SHG) signals from starch granules and type I collagen through a four-channel photon counting based Stokes-polarimeter. The 2D SHG images of samples are reconstructed using various polarization parameters, such as the degree of polarization (DOP), the degree of linear polarization (DOLP), the degree of circular polarization (DOCP), as well as the anisotropy from the acquired Stokes parameters. Furthermore, we have demonstrated that the polarization parameters are changes at different input polarizations and focusing depths.

AB - We analyzed the polarization states of second harmonic generation (SHG) signals from starch granules and type I collagen through a four-channel photon counting based Stokes-polarimeter. The 2D SHG images of samples are reconstructed using various polarization parameters, such as the degree of polarization (DOP), the degree of linear polarization (DOLP), the degree of circular polarization (DOCP), as well as the anisotropy from the acquired Stokes parameters. Furthermore, we have demonstrated that the polarization parameters are changes at different input polarizations and focusing depths.

UR - http://www.scopus.com/inward/record.url?scp=84878742143&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84878742143&partnerID=8YFLogxK

U2 - 10.1117/12.2003894

DO - 10.1117/12.2003894

M3 - Conference contribution

AN - SCOPUS:84878742143

SN - 9780819493576

VL - 8588

BT - Multiphoton Microscopy in the Biomedical Sciences XIII

T2 - Multiphoton Microscopy in the Biomedical Sciences XIII

Y2 - 3 February 2013 through 5 February 2013

ER -

Imaging molecular structure with Stokes-polarimeter based second harmonic generation microscopy

Abstract

Conference

All Science Journal Classification (ASJC) codes

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