Iminoflavones combat 1,2-dimethyl hydrazine-induced aberrant crypt foci development in colon cancer

V. Ganga Prasad, Shishir Kawade, B. S. Jayashree, Neetinkumar D. Reddy, Albi Francis, Pawan G. Nayak, Anoop Kishore, K. Nandakumar, C. Mallikarjuna Rao, Rekha R. Shenoy

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The aim of the present study was to evaluate the antitumor potential of iminoflavones in in vitro and in vivo anticancer models. Preliminary screening in various cancer cell lines revealed four potential iminoflavones out of which IMF-8 was taken based on its activity against colon cancer cells. This was further confirmed by observing the nuclear changes in the cells by AO/EB and Hoechst 33342 staining studies. In vivo activity was assessed by dimethyl hydrazine-(DMH-) induced colon cancer model in rats. Animals were administered DMH (20 mg/kg, b.w. for 10 weeks and 30 mg/kg b.w., i.p. for 10 weeks) and were supplemented with (IMF-8) iminoflavone-8 (200 mg/kg, p.o. for 14 days). Results showed that DMH induced 100% aberrant crypt foci (ACF) and polyps which were significantly reduced in the IMF-8 treated group. IMF-8 significantly increased the catalase and GSH levels whereas it reduced the TNF-alpha and IL-6 levels markedly which suggests the antioxidative and anti-inflammatory actions of flavonoids present in IMF-8. The histopathological images of the IMF-8 treated colon showed no signs of mucosal crypt abscess. These findings suggest that the semi-synthetic iminoflavones, IMF-8, effectively inhibit DMH-induced ACFs and colonic crypts by alleviating the oxidative stress and suppressing the inflammation.

Original languageEnglish
Article number569130
JournalBioMed Research International
Volume2014
DOIs
Publication statusPublished - 2014

Fingerprint

Dimenhydrinate
Aberrant Crypt Foci
1,2-Dimethylhydrazine
Colonic Neoplasms
Cells
Oxidative stress
Polyps
Flavonoids
Catalase
Abscess
Rats
Interleukin-6
Screening
Colon
Animals
Oxidative Stress
Anti-Inflammatory Agents
Tumor Necrosis Factor-alpha
Staining and Labeling
Inflammation

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)
  • Medicine(all)

Cite this

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title = "Iminoflavones combat 1,2-dimethyl hydrazine-induced aberrant crypt foci development in colon cancer",
abstract = "The aim of the present study was to evaluate the antitumor potential of iminoflavones in in vitro and in vivo anticancer models. Preliminary screening in various cancer cell lines revealed four potential iminoflavones out of which IMF-8 was taken based on its activity against colon cancer cells. This was further confirmed by observing the nuclear changes in the cells by AO/EB and Hoechst 33342 staining studies. In vivo activity was assessed by dimethyl hydrazine-(DMH-) induced colon cancer model in rats. Animals were administered DMH (20 mg/kg, b.w. for 10 weeks and 30 mg/kg b.w., i.p. for 10 weeks) and were supplemented with (IMF-8) iminoflavone-8 (200 mg/kg, p.o. for 14 days). Results showed that DMH induced 100{\%} aberrant crypt foci (ACF) and polyps which were significantly reduced in the IMF-8 treated group. IMF-8 significantly increased the catalase and GSH levels whereas it reduced the TNF-alpha and IL-6 levels markedly which suggests the antioxidative and anti-inflammatory actions of flavonoids present in IMF-8. The histopathological images of the IMF-8 treated colon showed no signs of mucosal crypt abscess. These findings suggest that the semi-synthetic iminoflavones, IMF-8, effectively inhibit DMH-induced ACFs and colonic crypts by alleviating the oxidative stress and suppressing the inflammation.",
author = "Prasad, {V. Ganga} and Shishir Kawade and Jayashree, {B. S.} and Reddy, {Neetinkumar D.} and Albi Francis and Nayak, {Pawan G.} and Anoop Kishore and K. Nandakumar and Rao, {C. Mallikarjuna} and Shenoy, {Rekha R.}",
year = "2014",
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journal = "BioMed Research International",
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Iminoflavones combat 1,2-dimethyl hydrazine-induced aberrant crypt foci development in colon cancer. / Prasad, V. Ganga; Kawade, Shishir; Jayashree, B. S.; Reddy, Neetinkumar D.; Francis, Albi; Nayak, Pawan G.; Kishore, Anoop; Nandakumar, K.; Rao, C. Mallikarjuna; Shenoy, Rekha R.

In: BioMed Research International, Vol. 2014, 569130, 2014.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Iminoflavones combat 1,2-dimethyl hydrazine-induced aberrant crypt foci development in colon cancer

AU - Prasad, V. Ganga

AU - Kawade, Shishir

AU - Jayashree, B. S.

AU - Reddy, Neetinkumar D.

AU - Francis, Albi

AU - Nayak, Pawan G.

AU - Kishore, Anoop

AU - Nandakumar, K.

AU - Rao, C. Mallikarjuna

AU - Shenoy, Rekha R.

PY - 2014

Y1 - 2014

N2 - The aim of the present study was to evaluate the antitumor potential of iminoflavones in in vitro and in vivo anticancer models. Preliminary screening in various cancer cell lines revealed four potential iminoflavones out of which IMF-8 was taken based on its activity against colon cancer cells. This was further confirmed by observing the nuclear changes in the cells by AO/EB and Hoechst 33342 staining studies. In vivo activity was assessed by dimethyl hydrazine-(DMH-) induced colon cancer model in rats. Animals were administered DMH (20 mg/kg, b.w. for 10 weeks and 30 mg/kg b.w., i.p. for 10 weeks) and were supplemented with (IMF-8) iminoflavone-8 (200 mg/kg, p.o. for 14 days). Results showed that DMH induced 100% aberrant crypt foci (ACF) and polyps which were significantly reduced in the IMF-8 treated group. IMF-8 significantly increased the catalase and GSH levels whereas it reduced the TNF-alpha and IL-6 levels markedly which suggests the antioxidative and anti-inflammatory actions of flavonoids present in IMF-8. The histopathological images of the IMF-8 treated colon showed no signs of mucosal crypt abscess. These findings suggest that the semi-synthetic iminoflavones, IMF-8, effectively inhibit DMH-induced ACFs and colonic crypts by alleviating the oxidative stress and suppressing the inflammation.

AB - The aim of the present study was to evaluate the antitumor potential of iminoflavones in in vitro and in vivo anticancer models. Preliminary screening in various cancer cell lines revealed four potential iminoflavones out of which IMF-8 was taken based on its activity against colon cancer cells. This was further confirmed by observing the nuclear changes in the cells by AO/EB and Hoechst 33342 staining studies. In vivo activity was assessed by dimethyl hydrazine-(DMH-) induced colon cancer model in rats. Animals were administered DMH (20 mg/kg, b.w. for 10 weeks and 30 mg/kg b.w., i.p. for 10 weeks) and were supplemented with (IMF-8) iminoflavone-8 (200 mg/kg, p.o. for 14 days). Results showed that DMH induced 100% aberrant crypt foci (ACF) and polyps which were significantly reduced in the IMF-8 treated group. IMF-8 significantly increased the catalase and GSH levels whereas it reduced the TNF-alpha and IL-6 levels markedly which suggests the antioxidative and anti-inflammatory actions of flavonoids present in IMF-8. The histopathological images of the IMF-8 treated colon showed no signs of mucosal crypt abscess. These findings suggest that the semi-synthetic iminoflavones, IMF-8, effectively inhibit DMH-induced ACFs and colonic crypts by alleviating the oxidative stress and suppressing the inflammation.

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