Abstract

BACKGROUND: Human variations related to immune response and disease susceptibility is well-documented in Ayurveda. Prakriti (body constitution) is the basic constitution of an individual established at the time of birth and distinguishes variations, into three broad phenotype categories such as vata, pitta and kapha. Variation in immune response is often attributed to and measured from the difference in cluster differentiation (CD) markers expressed in lymphocytes. Currently, there are no reports available on the expression of CD markers related to prakriti.

OBJECTIVE: This is a pilot study performed to evaluate a panel of lymphocyte subset CD markers in dominant prakriti individuals.

MATERIALS AND METHODS: Immunophenotyping was carried out using whole blood from a total of healthy 222 subjects, who are grouped into kapha (n = 95), pitta (n = 57) and vata (n = 70) prakritis. CD markers such as CD3, CD4, CD8, CD14, CD25, CD56, CD69, CD71 and HLA-DR were analyzed using flow cytometry method. Differences between groups were analyzed using one-way ANOVA or Kruskal-Wallis analysis of variance (ANOVA) and multiple comparisons between groups were performed by Bonferroni or Mann-Whitney U test with corrections for type I error respectively. Significance was evaluated by ANOVA and Pearson's correlation.

RESULTS: We observed a significant difference (P < 0.05) in the expression of CD markers such as CD14 (monocytes), CD25 (activated B cells) and CD56 (Natural killer cells) between different prakriti groups. CD25 and CD56 expression was significantly higher in kapha prakriti samples than other prakriti groups. Similarly, slightly higher levels of CD14 were observed in pitta prakriti samples.

CONCLUSION: Significant difference in the expression of CD14, CD25 and CD56 markers between three different prakriti is demonstrated. The increased level of CD25 and CD56 in kapha prakriti may indicate ability to elicit better immune response, which is in conformity with textual references in Ayurveda.

Original languageEnglish
Pages (from-to)43-9
Number of pages7
JournalJournal of Ayurveda and Integrative Medicine
Volume5
Issue number1
DOIs
Publication statusPublished - 01-2014

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CD Antigens
Immunophenotyping
Analysis of Variance
Body Constitution
Constitution and Bylaws
Disease Susceptibility
Immune System Diseases
Lymphocyte Subsets
HLA-DR Antigens
Nonparametric Statistics
Natural Killer Cells
Monocytes
Healthy Volunteers
Flow Cytometry
B-Lymphocytes
Parturition
Lymphocytes
Phenotype

Cite this

Rotti, Harish ; Guruprasad, Kanive Parashiva ; Nayak, Jayakrishna ; Kabekkodu, Shama Prasada ; Kukreja, Harpreet ; Mallya, Sandeep ; Nayak, Jyothi ; Bhradwaj, Ramachandra C ; Gangadharan, G G ; Prasanna, B V ; Raval, Ritu ; Kamath, Asha ; Gopinath, Puthiya Mundyat ; Kondaiah, Paturu ; Satyamoorthy, Kapaettu. / Immunophenotyping of normal individuals classified on the basis of human dosha prakriti. In: Journal of Ayurveda and Integrative Medicine. 2014 ; Vol. 5, No. 1. pp. 43-9.
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title = "Immunophenotyping of normal individuals classified on the basis of human dosha prakriti",
abstract = "BACKGROUND: Human variations related to immune response and disease susceptibility is well-documented in Ayurveda. Prakriti (body constitution) is the basic constitution of an individual established at the time of birth and distinguishes variations, into three broad phenotype categories such as vata, pitta and kapha. Variation in immune response is often attributed to and measured from the difference in cluster differentiation (CD) markers expressed in lymphocytes. Currently, there are no reports available on the expression of CD markers related to prakriti.OBJECTIVE: This is a pilot study performed to evaluate a panel of lymphocyte subset CD markers in dominant prakriti individuals.MATERIALS AND METHODS: Immunophenotyping was carried out using whole blood from a total of healthy 222 subjects, who are grouped into kapha (n = 95), pitta (n = 57) and vata (n = 70) prakritis. CD markers such as CD3, CD4, CD8, CD14, CD25, CD56, CD69, CD71 and HLA-DR were analyzed using flow cytometry method. Differences between groups were analyzed using one-way ANOVA or Kruskal-Wallis analysis of variance (ANOVA) and multiple comparisons between groups were performed by Bonferroni or Mann-Whitney U test with corrections for type I error respectively. Significance was evaluated by ANOVA and Pearson's correlation.RESULTS: We observed a significant difference (P < 0.05) in the expression of CD markers such as CD14 (monocytes), CD25 (activated B cells) and CD56 (Natural killer cells) between different prakriti groups. CD25 and CD56 expression was significantly higher in kapha prakriti samples than other prakriti groups. Similarly, slightly higher levels of CD14 were observed in pitta prakriti samples.CONCLUSION: Significant difference in the expression of CD14, CD25 and CD56 markers between three different prakriti is demonstrated. The increased level of CD25 and CD56 in kapha prakriti may indicate ability to elicit better immune response, which is in conformity with textual references in Ayurveda.",
author = "Harish Rotti and Guruprasad, {Kanive Parashiva} and Jayakrishna Nayak and Kabekkodu, {Shama Prasada} and Harpreet Kukreja and Sandeep Mallya and Jyothi Nayak and Bhradwaj, {Ramachandra C} and Gangadharan, {G G} and Prasanna, {B V} and Ritu Raval and Asha Kamath and Gopinath, {Puthiya Mundyat} and Paturu Kondaiah and Kapaettu Satyamoorthy",
year = "2014",
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language = "English",
volume = "5",
pages = "43--9",
journal = "Journal of Ayurveda and Integrative Medicine",
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Immunophenotyping of normal individuals classified on the basis of human dosha prakriti. / Rotti, Harish; Guruprasad, Kanive Parashiva; Nayak, Jayakrishna; Kabekkodu, Shama Prasada; Kukreja, Harpreet; Mallya, Sandeep; Nayak, Jyothi; Bhradwaj, Ramachandra C; Gangadharan, G G; Prasanna, B V; Raval, Ritu; Kamath, Asha; Gopinath, Puthiya Mundyat; Kondaiah, Paturu; Satyamoorthy, Kapaettu.

In: Journal of Ayurveda and Integrative Medicine, Vol. 5, No. 1, 01.2014, p. 43-9.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Immunophenotyping of normal individuals classified on the basis of human dosha prakriti

AU - Rotti, Harish

AU - Guruprasad, Kanive Parashiva

AU - Nayak, Jayakrishna

AU - Kabekkodu, Shama Prasada

AU - Kukreja, Harpreet

AU - Mallya, Sandeep

AU - Nayak, Jyothi

AU - Bhradwaj, Ramachandra C

AU - Gangadharan, G G

AU - Prasanna, B V

AU - Raval, Ritu

AU - Kamath, Asha

AU - Gopinath, Puthiya Mundyat

AU - Kondaiah, Paturu

AU - Satyamoorthy, Kapaettu

PY - 2014/1

Y1 - 2014/1

N2 - BACKGROUND: Human variations related to immune response and disease susceptibility is well-documented in Ayurveda. Prakriti (body constitution) is the basic constitution of an individual established at the time of birth and distinguishes variations, into three broad phenotype categories such as vata, pitta and kapha. Variation in immune response is often attributed to and measured from the difference in cluster differentiation (CD) markers expressed in lymphocytes. Currently, there are no reports available on the expression of CD markers related to prakriti.OBJECTIVE: This is a pilot study performed to evaluate a panel of lymphocyte subset CD markers in dominant prakriti individuals.MATERIALS AND METHODS: Immunophenotyping was carried out using whole blood from a total of healthy 222 subjects, who are grouped into kapha (n = 95), pitta (n = 57) and vata (n = 70) prakritis. CD markers such as CD3, CD4, CD8, CD14, CD25, CD56, CD69, CD71 and HLA-DR were analyzed using flow cytometry method. Differences between groups were analyzed using one-way ANOVA or Kruskal-Wallis analysis of variance (ANOVA) and multiple comparisons between groups were performed by Bonferroni or Mann-Whitney U test with corrections for type I error respectively. Significance was evaluated by ANOVA and Pearson's correlation.RESULTS: We observed a significant difference (P < 0.05) in the expression of CD markers such as CD14 (monocytes), CD25 (activated B cells) and CD56 (Natural killer cells) between different prakriti groups. CD25 and CD56 expression was significantly higher in kapha prakriti samples than other prakriti groups. Similarly, slightly higher levels of CD14 were observed in pitta prakriti samples.CONCLUSION: Significant difference in the expression of CD14, CD25 and CD56 markers between three different prakriti is demonstrated. The increased level of CD25 and CD56 in kapha prakriti may indicate ability to elicit better immune response, which is in conformity with textual references in Ayurveda.

AB - BACKGROUND: Human variations related to immune response and disease susceptibility is well-documented in Ayurveda. Prakriti (body constitution) is the basic constitution of an individual established at the time of birth and distinguishes variations, into three broad phenotype categories such as vata, pitta and kapha. Variation in immune response is often attributed to and measured from the difference in cluster differentiation (CD) markers expressed in lymphocytes. Currently, there are no reports available on the expression of CD markers related to prakriti.OBJECTIVE: This is a pilot study performed to evaluate a panel of lymphocyte subset CD markers in dominant prakriti individuals.MATERIALS AND METHODS: Immunophenotyping was carried out using whole blood from a total of healthy 222 subjects, who are grouped into kapha (n = 95), pitta (n = 57) and vata (n = 70) prakritis. CD markers such as CD3, CD4, CD8, CD14, CD25, CD56, CD69, CD71 and HLA-DR were analyzed using flow cytometry method. Differences between groups were analyzed using one-way ANOVA or Kruskal-Wallis analysis of variance (ANOVA) and multiple comparisons between groups were performed by Bonferroni or Mann-Whitney U test with corrections for type I error respectively. Significance was evaluated by ANOVA and Pearson's correlation.RESULTS: We observed a significant difference (P < 0.05) in the expression of CD markers such as CD14 (monocytes), CD25 (activated B cells) and CD56 (Natural killer cells) between different prakriti groups. CD25 and CD56 expression was significantly higher in kapha prakriti samples than other prakriti groups. Similarly, slightly higher levels of CD14 were observed in pitta prakriti samples.CONCLUSION: Significant difference in the expression of CD14, CD25 and CD56 markers between three different prakriti is demonstrated. The increased level of CD25 and CD56 in kapha prakriti may indicate ability to elicit better immune response, which is in conformity with textual references in Ayurveda.

U2 - 10.4103/0975-9476.128857

DO - 10.4103/0975-9476.128857

M3 - Article

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JO - Journal of Ayurveda and Integrative Medicine

JF - Journal of Ayurveda and Integrative Medicine

SN - 0975-9476

IS - 1

ER -