Impact of seasonal variant temperatures and laboratory room ambient temperature on mortality of rats with ischemic brain injury

Sivakumar Gopalakrishanan, M. G.Ramesh Babu, Rajesh Thangarajan, Dhiren Punja, Vidyadhara Devarunda Jaganath, Akriti B. Kanth, Mohandas Rao, Kiranmai S. Rai

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3 Citations (Scopus)

Abstract

Introduction: A popular rat model for hypoperfusion ischemic brain injury is bilateral common carotid artery occlusion (BCCAO). BCCAO surgery when performed in varying geographical locations and during different seasons of the year is reported to have variable mortality rates. Studies have also documented the diminishing influence of Ketamine-Xylazine (KT-XY) on thermoregulatory functions in rodents. Aim: To explore the impact of seasonal variant temperatures and laboratory room ambient temperatures on mortality of rats following BCCAO surgery. Materials and Methods: The study has two parts: 1 The first part is an analysis of a three year retrospective data to explore the association between the geographical season (hot summer and cold winter) induced laboratory room ambient temperature variations and the mortality rate in KT-XY anaesthetized BCCAO rats. 2. The second part investigated the effect of conditioned laboratory room ambient temperature (CAT) (23-25°C) in KT-XY anaesthetized BCCAO group of rats. Rats were divided into 4 groups(n =8/group) as-Normal control, BCCAO and Sham BCCAO where they were all exposed to unconditioned ambient temperature (UCAT) during their surgery and postoperative care. And finally fourth group rats exposed to CAT during the BCCAO surgery and postoperative care. Results: Pearson’s chi-square test indicates a significantly high association (p<0.006) between post-BCCAO mortality and hot season of the year. CAT during the hot season reduced the mortality rate (24% less) in post- BCCAO rats compared to the rats of UCAT. Conclusion: Despite seasonal variations in temperature, conditioning the laboratory room ambient temperatures to 23°C, induces hypothermia in KT-XY anaesthetized ischemic brain injured rodents and improves their survival rate.

Original languageEnglish
Pages (from-to)CF01-CF05
JournalJournal of Clinical and Diagnostic Research
Volume10
Issue number4
DOIs
Publication statusPublished - 01-04-2016

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Common Carotid Artery
Brain Injuries
Rats
Brain
Xylazine
Temperature
Mortality
Ketamine
Surgery
Postoperative Care
Hypothermia
Rodentia
Chi-Square Distribution
Survival Rate

All Science Journal Classification (ASJC) codes

  • Medicine(all)
  • Clinical Biochemistry

Cite this

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title = "Impact of seasonal variant temperatures and laboratory room ambient temperature on mortality of rats with ischemic brain injury",
abstract = "Introduction: A popular rat model for hypoperfusion ischemic brain injury is bilateral common carotid artery occlusion (BCCAO). BCCAO surgery when performed in varying geographical locations and during different seasons of the year is reported to have variable mortality rates. Studies have also documented the diminishing influence of Ketamine-Xylazine (KT-XY) on thermoregulatory functions in rodents. Aim: To explore the impact of seasonal variant temperatures and laboratory room ambient temperatures on mortality of rats following BCCAO surgery. Materials and Methods: The study has two parts: 1 The first part is an analysis of a three year retrospective data to explore the association between the geographical season (hot summer and cold winter) induced laboratory room ambient temperature variations and the mortality rate in KT-XY anaesthetized BCCAO rats. 2. The second part investigated the effect of conditioned laboratory room ambient temperature (CAT) (23-25°C) in KT-XY anaesthetized BCCAO group of rats. Rats were divided into 4 groups(n =8/group) as-Normal control, BCCAO and Sham BCCAO where they were all exposed to unconditioned ambient temperature (UCAT) during their surgery and postoperative care. And finally fourth group rats exposed to CAT during the BCCAO surgery and postoperative care. Results: Pearson’s chi-square test indicates a significantly high association (p<0.006) between post-BCCAO mortality and hot season of the year. CAT during the hot season reduced the mortality rate (24{\%} less) in post- BCCAO rats compared to the rats of UCAT. Conclusion: Despite seasonal variations in temperature, conditioning the laboratory room ambient temperatures to 23°C, induces hypothermia in KT-XY anaesthetized ischemic brain injured rodents and improves their survival rate.",
author = "Sivakumar Gopalakrishanan and Babu, {M. G.Ramesh} and Rajesh Thangarajan and Dhiren Punja and Jaganath, {Vidyadhara Devarunda} and Kanth, {Akriti B.} and Mohandas Rao and Rai, {Kiranmai S.}",
year = "2016",
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doi = "10.7860/JCDR/2016/17372.7597",
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TY - JOUR

T1 - Impact of seasonal variant temperatures and laboratory room ambient temperature on mortality of rats with ischemic brain injury

AU - Gopalakrishanan, Sivakumar

AU - Babu, M. G.Ramesh

AU - Thangarajan, Rajesh

AU - Punja, Dhiren

AU - Jaganath, Vidyadhara Devarunda

AU - Kanth, Akriti B.

AU - Rao, Mohandas

AU - Rai, Kiranmai S.

PY - 2016/4/1

Y1 - 2016/4/1

N2 - Introduction: A popular rat model for hypoperfusion ischemic brain injury is bilateral common carotid artery occlusion (BCCAO). BCCAO surgery when performed in varying geographical locations and during different seasons of the year is reported to have variable mortality rates. Studies have also documented the diminishing influence of Ketamine-Xylazine (KT-XY) on thermoregulatory functions in rodents. Aim: To explore the impact of seasonal variant temperatures and laboratory room ambient temperatures on mortality of rats following BCCAO surgery. Materials and Methods: The study has two parts: 1 The first part is an analysis of a three year retrospective data to explore the association between the geographical season (hot summer and cold winter) induced laboratory room ambient temperature variations and the mortality rate in KT-XY anaesthetized BCCAO rats. 2. The second part investigated the effect of conditioned laboratory room ambient temperature (CAT) (23-25°C) in KT-XY anaesthetized BCCAO group of rats. Rats were divided into 4 groups(n =8/group) as-Normal control, BCCAO and Sham BCCAO where they were all exposed to unconditioned ambient temperature (UCAT) during their surgery and postoperative care. And finally fourth group rats exposed to CAT during the BCCAO surgery and postoperative care. Results: Pearson’s chi-square test indicates a significantly high association (p<0.006) between post-BCCAO mortality and hot season of the year. CAT during the hot season reduced the mortality rate (24% less) in post- BCCAO rats compared to the rats of UCAT. Conclusion: Despite seasonal variations in temperature, conditioning the laboratory room ambient temperatures to 23°C, induces hypothermia in KT-XY anaesthetized ischemic brain injured rodents and improves their survival rate.

AB - Introduction: A popular rat model for hypoperfusion ischemic brain injury is bilateral common carotid artery occlusion (BCCAO). BCCAO surgery when performed in varying geographical locations and during different seasons of the year is reported to have variable mortality rates. Studies have also documented the diminishing influence of Ketamine-Xylazine (KT-XY) on thermoregulatory functions in rodents. Aim: To explore the impact of seasonal variant temperatures and laboratory room ambient temperatures on mortality of rats following BCCAO surgery. Materials and Methods: The study has two parts: 1 The first part is an analysis of a three year retrospective data to explore the association between the geographical season (hot summer and cold winter) induced laboratory room ambient temperature variations and the mortality rate in KT-XY anaesthetized BCCAO rats. 2. The second part investigated the effect of conditioned laboratory room ambient temperature (CAT) (23-25°C) in KT-XY anaesthetized BCCAO group of rats. Rats were divided into 4 groups(n =8/group) as-Normal control, BCCAO and Sham BCCAO where they were all exposed to unconditioned ambient temperature (UCAT) during their surgery and postoperative care. And finally fourth group rats exposed to CAT during the BCCAO surgery and postoperative care. Results: Pearson’s chi-square test indicates a significantly high association (p<0.006) between post-BCCAO mortality and hot season of the year. CAT during the hot season reduced the mortality rate (24% less) in post- BCCAO rats compared to the rats of UCAT. Conclusion: Despite seasonal variations in temperature, conditioning the laboratory room ambient temperatures to 23°C, induces hypothermia in KT-XY anaesthetized ischemic brain injured rodents and improves their survival rate.

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U2 - 10.7860/JCDR/2016/17372.7597

DO - 10.7860/JCDR/2016/17372.7597

M3 - Article

VL - 10

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JO - Journal of Clinical and Diagnostic Research

JF - Journal of Clinical and Diagnostic Research

SN - 2249-782X

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