Impact of wastewater from different sources on the prevalence of antimicrobial-resistant Escherichia coli in sewage treatment plants in South India

Masato Akiba, Hironobu Senba, Haruna Otagiri, Valipparambil P. Prabhasankar, Sachi Taniyasu, Nobuyoshi Yamashita, Ken ichi Lee, Takehisa Yamamoto, Toshiyuki Tsutsui, Derrick Ian Joshua, Keshava Balakrishna, Indira Bairy, Taketoshi Iwata, Masahiro Kusumoto, Kurunthachalam Kannan, Keerthi S. Guruge

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

The sewage treatment plant (STP) is one of the most important interfaces between the human population and the aquatic environment, leading to contamination of the latter by antimicrobial-resistant bacteria. To identify factors affecting the prevalence of antimicrobial-resistant bacteria, water samples were collected from three different STPs in South India. STP1 exclusively treats sewage generated by a domestic population. STP2 predominantly treats sewage generated by a domestic population with a mix of hospital effluent. STP3 treats effluents generated exclusively by a hospital. The water samples were collected between three intermediate treatment steps including equalization, aeration, and clarification, in addition to the outlet to assess the removal rates of bacteria as the effluent passed through the treatment plant. The samples were collected in three different seasons to study the effect of seasonal variation. Escherichia coli isolated from the water samples were tested for susceptibility to 12 antimicrobials. The results of logistic regression analysis suggest that the hospital wastewater inflow significantly increased the prevalence of antimicrobial-resistant E. coli, whereas the treatment processes and sampling seasons did not affect the prevalence of these isolates. A bias in the genotype distribution of E. coli was observed among the isolates obtained from STP3. In conclusion, hospital wastewaters should be carefully treated to prevent the contamination of Indian environment with antimicrobial-resistant bacteria.

Original languageEnglish
Pages (from-to)203-208
Number of pages6
JournalEcotoxicology and Environmental Safety
Volume115
DOIs
Publication statusPublished - 01-05-2015

Fingerprint

Sewage treatment plants
Waste Water
Sewage
Escherichia coli
India
Bacteria
Wastewater
Effluents
Water
Contamination
Population
Regression analysis
Logistics
Logistic Models
Genotype
Regression Analysis
Sampling

All Science Journal Classification (ASJC) codes

  • Pollution
  • Public Health, Environmental and Occupational Health
  • Health, Toxicology and Mutagenesis

Cite this

Akiba, Masato ; Senba, Hironobu ; Otagiri, Haruna ; Prabhasankar, Valipparambil P. ; Taniyasu, Sachi ; Yamashita, Nobuyoshi ; Lee, Ken ichi ; Yamamoto, Takehisa ; Tsutsui, Toshiyuki ; Ian Joshua, Derrick ; Balakrishna, Keshava ; Bairy, Indira ; Iwata, Taketoshi ; Kusumoto, Masahiro ; Kannan, Kurunthachalam ; Guruge, Keerthi S. / Impact of wastewater from different sources on the prevalence of antimicrobial-resistant Escherichia coli in sewage treatment plants in South India. In: Ecotoxicology and Environmental Safety. 2015 ; Vol. 115. pp. 203-208.
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abstract = "The sewage treatment plant (STP) is one of the most important interfaces between the human population and the aquatic environment, leading to contamination of the latter by antimicrobial-resistant bacteria. To identify factors affecting the prevalence of antimicrobial-resistant bacteria, water samples were collected from three different STPs in South India. STP1 exclusively treats sewage generated by a domestic population. STP2 predominantly treats sewage generated by a domestic population with a mix of hospital effluent. STP3 treats effluents generated exclusively by a hospital. The water samples were collected between three intermediate treatment steps including equalization, aeration, and clarification, in addition to the outlet to assess the removal rates of bacteria as the effluent passed through the treatment plant. The samples were collected in three different seasons to study the effect of seasonal variation. Escherichia coli isolated from the water samples were tested for susceptibility to 12 antimicrobials. The results of logistic regression analysis suggest that the hospital wastewater inflow significantly increased the prevalence of antimicrobial-resistant E. coli, whereas the treatment processes and sampling seasons did not affect the prevalence of these isolates. A bias in the genotype distribution of E. coli was observed among the isolates obtained from STP3. In conclusion, hospital wastewaters should be carefully treated to prevent the contamination of Indian environment with antimicrobial-resistant bacteria.",
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Akiba, M, Senba, H, Otagiri, H, Prabhasankar, VP, Taniyasu, S, Yamashita, N, Lee, KI, Yamamoto, T, Tsutsui, T, Ian Joshua, D, Balakrishna, K, Bairy, I, Iwata, T, Kusumoto, M, Kannan, K & Guruge, KS 2015, 'Impact of wastewater from different sources on the prevalence of antimicrobial-resistant Escherichia coli in sewage treatment plants in South India', Ecotoxicology and Environmental Safety, vol. 115, pp. 203-208. https://doi.org/10.1016/j.ecoenv.2015.02.018

Impact of wastewater from different sources on the prevalence of antimicrobial-resistant Escherichia coli in sewage treatment plants in South India. / Akiba, Masato; Senba, Hironobu; Otagiri, Haruna; Prabhasankar, Valipparambil P.; Taniyasu, Sachi; Yamashita, Nobuyoshi; Lee, Ken ichi; Yamamoto, Takehisa; Tsutsui, Toshiyuki; Ian Joshua, Derrick; Balakrishna, Keshava; Bairy, Indira; Iwata, Taketoshi; Kusumoto, Masahiro; Kannan, Kurunthachalam; Guruge, Keerthi S.

In: Ecotoxicology and Environmental Safety, Vol. 115, 01.05.2015, p. 203-208.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Impact of wastewater from different sources on the prevalence of antimicrobial-resistant Escherichia coli in sewage treatment plants in South India

AU - Akiba, Masato

AU - Senba, Hironobu

AU - Otagiri, Haruna

AU - Prabhasankar, Valipparambil P.

AU - Taniyasu, Sachi

AU - Yamashita, Nobuyoshi

AU - Lee, Ken ichi

AU - Yamamoto, Takehisa

AU - Tsutsui, Toshiyuki

AU - Ian Joshua, Derrick

AU - Balakrishna, Keshava

AU - Bairy, Indira

AU - Iwata, Taketoshi

AU - Kusumoto, Masahiro

AU - Kannan, Kurunthachalam

AU - Guruge, Keerthi S.

PY - 2015/5/1

Y1 - 2015/5/1

N2 - The sewage treatment plant (STP) is one of the most important interfaces between the human population and the aquatic environment, leading to contamination of the latter by antimicrobial-resistant bacteria. To identify factors affecting the prevalence of antimicrobial-resistant bacteria, water samples were collected from three different STPs in South India. STP1 exclusively treats sewage generated by a domestic population. STP2 predominantly treats sewage generated by a domestic population with a mix of hospital effluent. STP3 treats effluents generated exclusively by a hospital. The water samples were collected between three intermediate treatment steps including equalization, aeration, and clarification, in addition to the outlet to assess the removal rates of bacteria as the effluent passed through the treatment plant. The samples were collected in three different seasons to study the effect of seasonal variation. Escherichia coli isolated from the water samples were tested for susceptibility to 12 antimicrobials. The results of logistic regression analysis suggest that the hospital wastewater inflow significantly increased the prevalence of antimicrobial-resistant E. coli, whereas the treatment processes and sampling seasons did not affect the prevalence of these isolates. A bias in the genotype distribution of E. coli was observed among the isolates obtained from STP3. In conclusion, hospital wastewaters should be carefully treated to prevent the contamination of Indian environment with antimicrobial-resistant bacteria.

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