Importance of glucokinase -258G/A polymorphism in Asian Indians with post-transplant and type 2 diabetes mellitus

Imran Ali Khan, Kiran Kumar Vattam, Parveen Jahan, Qurratulain Hasan, Pragna Rao

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Type 2 diabetes mellitus (T2DM) and post-transplant diabetes mellitus (PTDM) are non-synonymous forms of diabetes. Glucokinase (GCK) plays a key role in glucose metabolism. The relationship between the GCK promoter and specific types of diabetes, such as PTDM and T2DM, in the Asian Indian population is unknown. We examined the occurrence of a specific GCK promoter variant (-258G/A) in patients with T2DM and PTDM. The case-control study enrolled 640 Asian Indian subjects, including controls (n = 250) and T2DM (n = 250), PTDM (n = 42), and non-post-transplant diabetes mellitus (non-PTDM) (n = 98) patients. Purified Deoxyribonucleic acid (DNA) was genotyped with the polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) analysis. The digested PCR products were analyzed on 12% polyacrylamide gels. The anthropometric, biochemical, and clinical details of each group were documented. GCK -258G/A alleles and genotypes were not associated with T2DM. However, among PTDM subjects, we detected a higher frequency of heterozygotes (52.4%) and a positive association with alleles/genotypes. The results suggest that the promoter region (-258G/A) of GCK plays an important role in PTDM in Asian Indians.

Original languageEnglish
Pages (from-to)25-30
Number of pages6
JournalIntractable and Rare Diseases Research
Volume5
Issue number1
DOIs
Publication statusPublished - 01-01-2016

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Glucokinase
Type 2 Diabetes Mellitus
Diabetes Mellitus
Transplants
Alleles
Genotype
Polymerase Chain Reaction
Heterozygote
Genetic Promoter Regions
Restriction Fragment Length Polymorphisms
Case-Control Studies
Glucose
DNA

All Science Journal Classification (ASJC) codes

  • Medicine(all)

Cite this

Khan, Imran Ali ; Vattam, Kiran Kumar ; Jahan, Parveen ; Hasan, Qurratulain ; Rao, Pragna. / Importance of glucokinase -258G/A polymorphism in Asian Indians with post-transplant and type 2 diabetes mellitus. In: Intractable and Rare Diseases Research. 2016 ; Vol. 5, No. 1. pp. 25-30.
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Importance of glucokinase -258G/A polymorphism in Asian Indians with post-transplant and type 2 diabetes mellitus. / Khan, Imran Ali; Vattam, Kiran Kumar; Jahan, Parveen; Hasan, Qurratulain; Rao, Pragna.

In: Intractable and Rare Diseases Research, Vol. 5, No. 1, 01.01.2016, p. 25-30.

Research output: Contribution to journalArticle

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