Improvement of the Production and Characterisation of Polyhydroxyalkanoate by Bacillus endophyticus Using Inexpensive Carbon Feedstock

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Abstract

The study aimed at the production and characterization of biopolymer polyhydroxyalkanoate (PHA) by Bacillus endophyticus. The usage of refined (sucrose 2–4%) and inexpensive unrefined (sugar cane molasses 2–4%) carbon source was evaluated by varying the media components via shake flask method and batch cultivation in bioreactor. The optimal PHA production of 10.7 g/L of PHA from 15.37 g/L of cell density was obtained using 4% sugarcane molasses (SCM) at 250 rpm using optimized medium obtained through statistical experimental design. Batch cultivation with increased agitation rate and addition of feeding nutrients provided by SCM significantly lead to enhanced PHA production by the test organism by increasing the overall cell density. According to the statistical model, the increase in the sugar concentration (> 4%) by SCM had a negative effect on PHA production. Characterization by FTIR and 1 H NMR was performed to confirm the biopolymer that has been produced by Bacillus spp.

Original languageEnglish
Pages (from-to)917-928
Number of pages12
JournalJournal of Polymers and the Environment
Volume27
Issue number5
DOIs
Publication statusPublished - 01-05-2019

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Polyhydroxyalkanoates
Molasses
Bacilli
Feedstocks
Carbon
Biopolymers
Sugar (sucrose)
Sugar cane
Bioreactors
Design of experiments
Nutrients
Nuclear magnetic resonance
Sucrose

All Science Journal Classification (ASJC) codes

  • Environmental Engineering
  • Polymers and Plastics
  • Materials Chemistry

Cite this

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title = "Improvement of the Production and Characterisation of Polyhydroxyalkanoate by Bacillus endophyticus Using Inexpensive Carbon Feedstock",
abstract = "The study aimed at the production and characterization of biopolymer polyhydroxyalkanoate (PHA) by Bacillus endophyticus. The usage of refined (sucrose 2–4{\%}) and inexpensive unrefined (sugar cane molasses 2–4{\%}) carbon source was evaluated by varying the media components via shake flask method and batch cultivation in bioreactor. The optimal PHA production of 10.7 g/L of PHA from 15.37 g/L of cell density was obtained using 4{\%} sugarcane molasses (SCM) at 250 rpm using optimized medium obtained through statistical experimental design. Batch cultivation with increased agitation rate and addition of feeding nutrients provided by SCM significantly lead to enhanced PHA production by the test organism by increasing the overall cell density. According to the statistical model, the increase in the sugar concentration (> 4{\%}) by SCM had a negative effect on PHA production. Characterization by FTIR and 1 H NMR was performed to confirm the biopolymer that has been produced by Bacillus spp.",
author = "M. Geethu and R. Vrundha and S. Raja and {Raghu Chandrashekar}, H. and Divyashree, {M. S.}",
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AU - Geethu, M.

AU - Vrundha, R.

AU - Raja, S.

AU - Raghu Chandrashekar, H.

AU - Divyashree, M. S.

PY - 2019/5/1

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AB - The study aimed at the production and characterization of biopolymer polyhydroxyalkanoate (PHA) by Bacillus endophyticus. The usage of refined (sucrose 2–4%) and inexpensive unrefined (sugar cane molasses 2–4%) carbon source was evaluated by varying the media components via shake flask method and batch cultivation in bioreactor. The optimal PHA production of 10.7 g/L of PHA from 15.37 g/L of cell density was obtained using 4% sugarcane molasses (SCM) at 250 rpm using optimized medium obtained through statistical experimental design. Batch cultivation with increased agitation rate and addition of feeding nutrients provided by SCM significantly lead to enhanced PHA production by the test organism by increasing the overall cell density. According to the statistical model, the increase in the sugar concentration (> 4%) by SCM had a negative effect on PHA production. Characterization by FTIR and 1 H NMR was performed to confirm the biopolymer that has been produced by Bacillus spp.

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