In pixel analysis of molecular structure with Stokes vector resolved second harmonic generation microscopy

Nirmal Mazumder; Lu Yun Xiang; Jianjun Qiu; Fu Jen Kao

doi:10.1117/12.2036651

In pixel analysis of molecular structure with Stokes vector resolved second harmonic generation microscopy

Nirmal Mazumder, Lu Yun Xiang, Jianjun Qiu, Fu Jen Kao

Department of Biophysics, Manipal School of Life Sciences, Manipal

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

3 Citations (Scopus)

Abstract

We report on measurements and characterization of polarization properties of Second Harmonic (SH) signals using a four-channel photon counting based Stokes polarimeter from type I collagen and starch granules. In this way, the critical polarization parameters including the degree of polarization (DOP), the degree of linear polarization (DOLP), and the degree of circular polarization (DOCP), are extracted from the reconstructed Stokes vector based SH images in a pixel-by-pixel manner. The measurements are further extended to determine the molecular structure and orientation of the samples by varying the polarization states of the incident light and recording the resulting Stokes parameters of the SH signal. The combination of SHG microscopy and Stokes polarimeter hence makes a powerful tool to investigate the structural order of starch granules under water and heating environment.

Original language	English
Title of host publication	Multiphoton Microscopy in the Biomedical Sciences XIV
Publisher	SPIE
Volume	8948
ISBN (Print)	9780819498618
DOIs	https://doi.org/10.1117/12.2036651
Publication status	Published - 2014
Event	Multiphoton Microscopy in the Biomedical Sciences XIV - San Francisco, CA, United States Duration: 02-02-2014 → 04-02-2014

Conference

Conference	Multiphoton Microscopy in the Biomedical Sciences XIV
Country/Territory	United States
City	San Francisco, CA
Period	02-02-14 → 04-02-14

All Science Journal Classification (ASJC) codes

Atomic and Molecular Physics, and Optics
Electronic, Optical and Magnetic Materials
Biomaterials
Radiology Nuclear Medicine and imaging

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10.1117/12.2036651

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@inproceedings{c90339c5b9944c4e91e61c4960dd9088,

title = "In pixel analysis of molecular structure with Stokes vector resolved second harmonic generation microscopy",

abstract = "We report on measurements and characterization of polarization properties of Second Harmonic (SH) signals using a four-channel photon counting based Stokes polarimeter from type I collagen and starch granules. In this way, the critical polarization parameters including the degree of polarization (DOP), the degree of linear polarization (DOLP), and the degree of circular polarization (DOCP), are extracted from the reconstructed Stokes vector based SH images in a pixel-by-pixel manner. The measurements are further extended to determine the molecular structure and orientation of the samples by varying the polarization states of the incident light and recording the resulting Stokes parameters of the SH signal. The combination of SHG microscopy and Stokes polarimeter hence makes a powerful tool to investigate the structural order of starch granules under water and heating environment.",

author = "Nirmal Mazumder and Xiang, {Lu Yun} and Jianjun Qiu and Kao, {Fu Jen}",

year = "2014",

doi = "10.1117/12.2036651",

language = "English",

isbn = "9780819498618",

volume = "8948",

booktitle = "Multiphoton Microscopy in the Biomedical Sciences XIV",

publisher = "SPIE",

address = "United States",

note = "Multiphoton Microscopy in the Biomedical Sciences XIV ; Conference date: 02-02-2014 Through 04-02-2014",

}

Mazumder, N, Xiang, LY, Qiu, J & Kao, FJ 2014, In pixel analysis of molecular structure with Stokes vector resolved second harmonic generation microscopy. in Multiphoton Microscopy in the Biomedical Sciences XIV. vol. 8948, 894822, SPIE, Multiphoton Microscopy in the Biomedical Sciences XIV, San Francisco, CA, United States, 02-02-14. https://doi.org/10.1117/12.2036651

TY - GEN

T1 - In pixel analysis of molecular structure with Stokes vector resolved second harmonic generation microscopy

AU - Mazumder, Nirmal

AU - Xiang, Lu Yun

AU - Qiu, Jianjun

AU - Kao, Fu Jen

PY - 2014

Y1 - 2014

N2 - We report on measurements and characterization of polarization properties of Second Harmonic (SH) signals using a four-channel photon counting based Stokes polarimeter from type I collagen and starch granules. In this way, the critical polarization parameters including the degree of polarization (DOP), the degree of linear polarization (DOLP), and the degree of circular polarization (DOCP), are extracted from the reconstructed Stokes vector based SH images in a pixel-by-pixel manner. The measurements are further extended to determine the molecular structure and orientation of the samples by varying the polarization states of the incident light and recording the resulting Stokes parameters of the SH signal. The combination of SHG microscopy and Stokes polarimeter hence makes a powerful tool to investigate the structural order of starch granules under water and heating environment.

AB - We report on measurements and characterization of polarization properties of Second Harmonic (SH) signals using a four-channel photon counting based Stokes polarimeter from type I collagen and starch granules. In this way, the critical polarization parameters including the degree of polarization (DOP), the degree of linear polarization (DOLP), and the degree of circular polarization (DOCP), are extracted from the reconstructed Stokes vector based SH images in a pixel-by-pixel manner. The measurements are further extended to determine the molecular structure and orientation of the samples by varying the polarization states of the incident light and recording the resulting Stokes parameters of the SH signal. The combination of SHG microscopy and Stokes polarimeter hence makes a powerful tool to investigate the structural order of starch granules under water and heating environment.

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U2 - 10.1117/12.2036651

DO - 10.1117/12.2036651

M3 - Conference contribution

AN - SCOPUS:84898022863

SN - 9780819498618

VL - 8948

BT - Multiphoton Microscopy in the Biomedical Sciences XIV

PB - SPIE

T2 - Multiphoton Microscopy in the Biomedical Sciences XIV

Y2 - 2 February 2014 through 4 February 2014

ER -

In pixel analysis of molecular structure with Stokes vector resolved second harmonic generation microscopy

Abstract

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All Science Journal Classification (ASJC) codes

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