In pixel analysis of molecular structure with Stokes vector resolved second harmonic generation microscopy

Nirmal Mazumder, Lu Yun Xiang, Jianjun Qiu, Fu Jen Kao

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

We report on measurements and characterization of polarization properties of Second Harmonic (SH) signals using a four-channel photon counting based Stokes polarimeter from type I collagen and starch granules. In this way, the critical polarization parameters including the degree of polarization (DOP), the degree of linear polarization (DOLP), and the degree of circular polarization (DOCP), are extracted from the reconstructed Stokes vector based SH images in a pixel-by-pixel manner. The measurements are further extended to determine the molecular structure and orientation of the samples by varying the polarization states of the incident light and recording the resulting Stokes parameters of the SH signal. The combination of SHG microscopy and Stokes polarimeter hence makes a powerful tool to investigate the structural order of starch granules under water and heating environment.

Original languageEnglish
Title of host publicationMultiphoton Microscopy in the Biomedical Sciences XIV
PublisherSPIE
Volume8948
ISBN (Print)9780819498618
DOIs
Publication statusPublished - 2014
EventMultiphoton Microscopy in the Biomedical Sciences XIV - San Francisco, CA, United States
Duration: 02-02-201404-02-2014

Conference

ConferenceMultiphoton Microscopy in the Biomedical Sciences XIV
CountryUnited States
CitySan Francisco, CA
Period02-02-1404-02-14

Fingerprint

Harmonic generation
Molecular Structure
Starch
Molecular structure
Microscopy
harmonic generations
Microscopic examination
molecular structure
Pixels
pixels
Polarization
microscopy
starches
polarimeters
polarization
Collagen Type I
Photons
harmonics
Polarimeters
Heating

All Science Journal Classification (ASJC) codes

  • Atomic and Molecular Physics, and Optics
  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Radiology Nuclear Medicine and imaging

Cite this

Mazumder, N., Xiang, L. Y., Qiu, J., & Kao, F. J. (2014). In pixel analysis of molecular structure with Stokes vector resolved second harmonic generation microscopy. In Multiphoton Microscopy in the Biomedical Sciences XIV (Vol. 8948). [894822] SPIE. https://doi.org/10.1117/12.2036651
Mazumder, Nirmal ; Xiang, Lu Yun ; Qiu, Jianjun ; Kao, Fu Jen. / In pixel analysis of molecular structure with Stokes vector resolved second harmonic generation microscopy. Multiphoton Microscopy in the Biomedical Sciences XIV. Vol. 8948 SPIE, 2014.
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abstract = "We report on measurements and characterization of polarization properties of Second Harmonic (SH) signals using a four-channel photon counting based Stokes polarimeter from type I collagen and starch granules. In this way, the critical polarization parameters including the degree of polarization (DOP), the degree of linear polarization (DOLP), and the degree of circular polarization (DOCP), are extracted from the reconstructed Stokes vector based SH images in a pixel-by-pixel manner. The measurements are further extended to determine the molecular structure and orientation of the samples by varying the polarization states of the incident light and recording the resulting Stokes parameters of the SH signal. The combination of SHG microscopy and Stokes polarimeter hence makes a powerful tool to investigate the structural order of starch granules under water and heating environment.",
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Mazumder, N, Xiang, LY, Qiu, J & Kao, FJ 2014, In pixel analysis of molecular structure with Stokes vector resolved second harmonic generation microscopy. in Multiphoton Microscopy in the Biomedical Sciences XIV. vol. 8948, 894822, SPIE, Multiphoton Microscopy in the Biomedical Sciences XIV, San Francisco, CA, United States, 02-02-14. https://doi.org/10.1117/12.2036651

In pixel analysis of molecular structure with Stokes vector resolved second harmonic generation microscopy. / Mazumder, Nirmal; Xiang, Lu Yun; Qiu, Jianjun; Kao, Fu Jen.

Multiphoton Microscopy in the Biomedical Sciences XIV. Vol. 8948 SPIE, 2014. 894822.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

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AU - Mazumder, Nirmal

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N2 - We report on measurements and characterization of polarization properties of Second Harmonic (SH) signals using a four-channel photon counting based Stokes polarimeter from type I collagen and starch granules. In this way, the critical polarization parameters including the degree of polarization (DOP), the degree of linear polarization (DOLP), and the degree of circular polarization (DOCP), are extracted from the reconstructed Stokes vector based SH images in a pixel-by-pixel manner. The measurements are further extended to determine the molecular structure and orientation of the samples by varying the polarization states of the incident light and recording the resulting Stokes parameters of the SH signal. The combination of SHG microscopy and Stokes polarimeter hence makes a powerful tool to investigate the structural order of starch granules under water and heating environment.

AB - We report on measurements and characterization of polarization properties of Second Harmonic (SH) signals using a four-channel photon counting based Stokes polarimeter from type I collagen and starch granules. In this way, the critical polarization parameters including the degree of polarization (DOP), the degree of linear polarization (DOLP), and the degree of circular polarization (DOCP), are extracted from the reconstructed Stokes vector based SH images in a pixel-by-pixel manner. The measurements are further extended to determine the molecular structure and orientation of the samples by varying the polarization states of the incident light and recording the resulting Stokes parameters of the SH signal. The combination of SHG microscopy and Stokes polarimeter hence makes a powerful tool to investigate the structural order of starch granules under water and heating environment.

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Mazumder N, Xiang LY, Qiu J, Kao FJ. In pixel analysis of molecular structure with Stokes vector resolved second harmonic generation microscopy. In Multiphoton Microscopy in the Biomedical Sciences XIV. Vol. 8948. SPIE. 2014. 894822 https://doi.org/10.1117/12.2036651