In vitro activity of vancomycin and daptomycin against healthcare-associated methicillin-resistant staphylococcus Aureus isolated from clinical specimens

Jyoti Kumari, M. Shalini Shenoy, M. Chakrapani, K. Vidyalakshmi, K. Gopalkrishna Bhat

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Abstract

Objective: The present cross-sectional study was conducted to determine minimum inhibitory concentration (MIC) of daptomycin and vancomycin to clinical isolates of healthcare-associated-methicillin-resistant Staphylococcus aureus (HA-MRSA). Methods: Centers for Disease Control and Prevention Criteria were used to define HR infections due to MRSA. Antibiotic susceptibility testing was done by Kirby–Bauer disk diffusion method. MIC of vancomycin and daptomycin was determined by Agar dilution method and E-test, respectively. Results of antibiotic susceptibility testing and MIC were interpreted as per Clinical Laboratory Standard Institute guidelines. Results: A total of 110 strains of MRSA were isolated from healthcare-associated infections. All were susceptible to daptomycin, linezolid, and teicoplanin. A total of 106 isolates were vancomycin susceptible and four were vancomycin-intermediate S. aureus (VISA). MIC90and MIC50of vancomycin were 2 μg/ml. All MRSA isolates were susceptible to daptomycin. Four VISA strains had daptomycin MIC 1 μg/ml. Conclusion: The present study showed the emergence of VISA among HA-MRSA isolates with high MIC90for vancomycin. Although all HA-MRSA isolates were susceptible to daptomycin, VISA isolates had high daptomycin MIC. This indicates that daptomycin may not be used as an alternative choice for VISA infections.

Original languageEnglish
JournalAsian Journal of Pharmaceutical and Clinical Research
Volume9
Issue number3
Publication statusPublished - 01-05-2016

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Daptomycin
Vancomycin
Methicillin-Resistant Staphylococcus aureus
Delivery of Health Care
Microbial Sensitivity Tests
Staphylococcus aureus
Linezolid
In Vitro Techniques
Anti-Bacterial Agents
Teicoplanin
Centers for Disease Control and Prevention (U.S.)
Cross Infection
Infection
Agar
Cross-Sectional Studies
Guidelines

All Science Journal Classification (ASJC) codes

  • Pharmacology
  • Pharmaceutical Science
  • Pharmacology (medical)

Cite this

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title = "In vitro activity of vancomycin and daptomycin against healthcare-associated methicillin-resistant staphylococcus Aureus isolated from clinical specimens",
abstract = "Objective: The present cross-sectional study was conducted to determine minimum inhibitory concentration (MIC) of daptomycin and vancomycin to clinical isolates of healthcare-associated-methicillin-resistant Staphylococcus aureus (HA-MRSA). Methods: Centers for Disease Control and Prevention Criteria were used to define HR infections due to MRSA. Antibiotic susceptibility testing was done by Kirby–Bauer disk diffusion method. MIC of vancomycin and daptomycin was determined by Agar dilution method and E-test, respectively. Results of antibiotic susceptibility testing and MIC were interpreted as per Clinical Laboratory Standard Institute guidelines. Results: A total of 110 strains of MRSA were isolated from healthcare-associated infections. All were susceptible to daptomycin, linezolid, and teicoplanin. A total of 106 isolates were vancomycin susceptible and four were vancomycin-intermediate S. aureus (VISA). MIC90and MIC50of vancomycin were 2 μg/ml. All MRSA isolates were susceptible to daptomycin. Four VISA strains had daptomycin MIC 1 μg/ml. Conclusion: The present study showed the emergence of VISA among HA-MRSA isolates with high MIC90for vancomycin. Although all HA-MRSA isolates were susceptible to daptomycin, VISA isolates had high daptomycin MIC. This indicates that daptomycin may not be used as an alternative choice for VISA infections.",
author = "Jyoti Kumari and {Shalini Shenoy}, M. and M. Chakrapani and K. Vidyalakshmi and {Gopalkrishna Bhat}, K.",
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T1 - In vitro activity of vancomycin and daptomycin against healthcare-associated methicillin-resistant staphylococcus Aureus isolated from clinical specimens

AU - Kumari, Jyoti

AU - Shalini Shenoy, M.

AU - Chakrapani, M.

AU - Vidyalakshmi, K.

AU - Gopalkrishna Bhat, K.

PY - 2016/5/1

Y1 - 2016/5/1

N2 - Objective: The present cross-sectional study was conducted to determine minimum inhibitory concentration (MIC) of daptomycin and vancomycin to clinical isolates of healthcare-associated-methicillin-resistant Staphylococcus aureus (HA-MRSA). Methods: Centers for Disease Control and Prevention Criteria were used to define HR infections due to MRSA. Antibiotic susceptibility testing was done by Kirby–Bauer disk diffusion method. MIC of vancomycin and daptomycin was determined by Agar dilution method and E-test, respectively. Results of antibiotic susceptibility testing and MIC were interpreted as per Clinical Laboratory Standard Institute guidelines. Results: A total of 110 strains of MRSA were isolated from healthcare-associated infections. All were susceptible to daptomycin, linezolid, and teicoplanin. A total of 106 isolates were vancomycin susceptible and four were vancomycin-intermediate S. aureus (VISA). MIC90and MIC50of vancomycin were 2 μg/ml. All MRSA isolates were susceptible to daptomycin. Four VISA strains had daptomycin MIC 1 μg/ml. Conclusion: The present study showed the emergence of VISA among HA-MRSA isolates with high MIC90for vancomycin. Although all HA-MRSA isolates were susceptible to daptomycin, VISA isolates had high daptomycin MIC. This indicates that daptomycin may not be used as an alternative choice for VISA infections.

AB - Objective: The present cross-sectional study was conducted to determine minimum inhibitory concentration (MIC) of daptomycin and vancomycin to clinical isolates of healthcare-associated-methicillin-resistant Staphylococcus aureus (HA-MRSA). Methods: Centers for Disease Control and Prevention Criteria were used to define HR infections due to MRSA. Antibiotic susceptibility testing was done by Kirby–Bauer disk diffusion method. MIC of vancomycin and daptomycin was determined by Agar dilution method and E-test, respectively. Results of antibiotic susceptibility testing and MIC were interpreted as per Clinical Laboratory Standard Institute guidelines. Results: A total of 110 strains of MRSA were isolated from healthcare-associated infections. All were susceptible to daptomycin, linezolid, and teicoplanin. A total of 106 isolates were vancomycin susceptible and four were vancomycin-intermediate S. aureus (VISA). MIC90and MIC50of vancomycin were 2 μg/ml. All MRSA isolates were susceptible to daptomycin. Four VISA strains had daptomycin MIC 1 μg/ml. Conclusion: The present study showed the emergence of VISA among HA-MRSA isolates with high MIC90for vancomycin. Although all HA-MRSA isolates were susceptible to daptomycin, VISA isolates had high daptomycin MIC. This indicates that daptomycin may not be used as an alternative choice for VISA infections.

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