In vitro and in vivo assessment of novel pH-sensitive interpenetrating polymer networks of a graft copolymer for gastro-protective delivery of ketoprofen

Rashmi Boppana, Raghavendra V. Kulkarni, G. Krishna Mohan, Srinivas Mutalik, Tejraj M. Aminabhavi

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The pH-sensitive polyacrylamide-grafted-tamarind seed polysaccharide (PAam-g-TSP) copolymer was synthesized under microwave irradiation in the presence of ceric ammonium nitrate in order to initiate the reaction. The reaction mixture was subjected to hydrolysis in alkaline medium. The copolymer was characterized with the help of FTIR, 1H-NMR and elemental analysis techniques. Furthermore, pH-sensitive IPN microbeads were prepared from these polymers in combination with sodium alginate through a dual crosslinking process and used for gastro-protective delivery of ketoprofen. The IPN microbeads were tested in vitro and in vivo and showed pH-dependent swelling/shrinking with the changing pH of the medium. Drug release was higher in 7.4 pH medium (about 83%), but it was lower in pH 1.2 medium (about 17%). The in vivo pharmacokinetics, anti-inflammatory activity and stomach histopathological evaluation performed on Wistar rats suggested that the microbeads are pH-sensitive and they were able to target ketoprofen to the intestine, resulting in diminished adverse effects in the stomach.

Original languageEnglish
Pages (from-to)64344-64356
Number of pages13
JournalRSC Advances
Volume6
Issue number69
DOIs
Publication statusPublished - 2016

Fingerprint

Ketoprofen
Interpenetrating polymer networks
Graft copolymers
Copolymers
Sodium alginate
Pharmacokinetics
Microwave irradiation
Polysaccharides
Polyacrylates
Crosslinking
Swelling
Seed
Rats
Nitrates
Hydrolysis
Polymers
Anti-Inflammatory Agents
Nuclear magnetic resonance
Chemical analysis
Pharmaceutical Preparations

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Chemical Engineering(all)

Cite this

Boppana, Rashmi ; Kulkarni, Raghavendra V. ; Mohan, G. Krishna ; Mutalik, Srinivas ; Aminabhavi, Tejraj M. / In vitro and in vivo assessment of novel pH-sensitive interpenetrating polymer networks of a graft copolymer for gastro-protective delivery of ketoprofen. In: RSC Advances. 2016 ; Vol. 6, No. 69. pp. 64344-64356.
@article{5c3fe5025d4b4c7b9b9016d1647ba09d,
title = "In vitro and in vivo assessment of novel pH-sensitive interpenetrating polymer networks of a graft copolymer for gastro-protective delivery of ketoprofen",
abstract = "The pH-sensitive polyacrylamide-grafted-tamarind seed polysaccharide (PAam-g-TSP) copolymer was synthesized under microwave irradiation in the presence of ceric ammonium nitrate in order to initiate the reaction. The reaction mixture was subjected to hydrolysis in alkaline medium. The copolymer was characterized with the help of FTIR, 1H-NMR and elemental analysis techniques. Furthermore, pH-sensitive IPN microbeads were prepared from these polymers in combination with sodium alginate through a dual crosslinking process and used for gastro-protective delivery of ketoprofen. The IPN microbeads were tested in vitro and in vivo and showed pH-dependent swelling/shrinking with the changing pH of the medium. Drug release was higher in 7.4 pH medium (about 83{\%}), but it was lower in pH 1.2 medium (about 17{\%}). The in vivo pharmacokinetics, anti-inflammatory activity and stomach histopathological evaluation performed on Wistar rats suggested that the microbeads are pH-sensitive and they were able to target ketoprofen to the intestine, resulting in diminished adverse effects in the stomach.",
author = "Rashmi Boppana and Kulkarni, {Raghavendra V.} and Mohan, {G. Krishna} and Srinivas Mutalik and Aminabhavi, {Tejraj M.}",
year = "2016",
doi = "10.1039/c6ra04218j",
language = "English",
volume = "6",
pages = "64344--64356",
journal = "RSC Advances",
issn = "2046-2069",
publisher = "Royal Society of Chemistry",
number = "69",

}

In vitro and in vivo assessment of novel pH-sensitive interpenetrating polymer networks of a graft copolymer for gastro-protective delivery of ketoprofen. / Boppana, Rashmi; Kulkarni, Raghavendra V.; Mohan, G. Krishna; Mutalik, Srinivas; Aminabhavi, Tejraj M.

In: RSC Advances, Vol. 6, No. 69, 2016, p. 64344-64356.

Research output: Contribution to journalArticle

TY - JOUR

T1 - In vitro and in vivo assessment of novel pH-sensitive interpenetrating polymer networks of a graft copolymer for gastro-protective delivery of ketoprofen

AU - Boppana, Rashmi

AU - Kulkarni, Raghavendra V.

AU - Mohan, G. Krishna

AU - Mutalik, Srinivas

AU - Aminabhavi, Tejraj M.

PY - 2016

Y1 - 2016

N2 - The pH-sensitive polyacrylamide-grafted-tamarind seed polysaccharide (PAam-g-TSP) copolymer was synthesized under microwave irradiation in the presence of ceric ammonium nitrate in order to initiate the reaction. The reaction mixture was subjected to hydrolysis in alkaline medium. The copolymer was characterized with the help of FTIR, 1H-NMR and elemental analysis techniques. Furthermore, pH-sensitive IPN microbeads were prepared from these polymers in combination with sodium alginate through a dual crosslinking process and used for gastro-protective delivery of ketoprofen. The IPN microbeads were tested in vitro and in vivo and showed pH-dependent swelling/shrinking with the changing pH of the medium. Drug release was higher in 7.4 pH medium (about 83%), but it was lower in pH 1.2 medium (about 17%). The in vivo pharmacokinetics, anti-inflammatory activity and stomach histopathological evaluation performed on Wistar rats suggested that the microbeads are pH-sensitive and they were able to target ketoprofen to the intestine, resulting in diminished adverse effects in the stomach.

AB - The pH-sensitive polyacrylamide-grafted-tamarind seed polysaccharide (PAam-g-TSP) copolymer was synthesized under microwave irradiation in the presence of ceric ammonium nitrate in order to initiate the reaction. The reaction mixture was subjected to hydrolysis in alkaline medium. The copolymer was characterized with the help of FTIR, 1H-NMR and elemental analysis techniques. Furthermore, pH-sensitive IPN microbeads were prepared from these polymers in combination with sodium alginate through a dual crosslinking process and used for gastro-protective delivery of ketoprofen. The IPN microbeads were tested in vitro and in vivo and showed pH-dependent swelling/shrinking with the changing pH of the medium. Drug release was higher in 7.4 pH medium (about 83%), but it was lower in pH 1.2 medium (about 17%). The in vivo pharmacokinetics, anti-inflammatory activity and stomach histopathological evaluation performed on Wistar rats suggested that the microbeads are pH-sensitive and they were able to target ketoprofen to the intestine, resulting in diminished adverse effects in the stomach.

UR - http://www.scopus.com/inward/record.url?scp=84978829776&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84978829776&partnerID=8YFLogxK

U2 - 10.1039/c6ra04218j

DO - 10.1039/c6ra04218j

M3 - Article

AN - SCOPUS:84978829776

VL - 6

SP - 64344

EP - 64356

JO - RSC Advances

JF - RSC Advances

SN - 2046-2069

IS - 69

ER -