In-vitro and in-vivo evaluation of novel injectable contraceptive preparations of Centchroman

B. Dinesh Shenoy, N. Udupa, U. V. Singh, Nagaraj Kumari

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Objectives: The in vitro release profile, stability and anti-fertility efficacy of some injectable, biodegradable formulations of Centchroman was investigated. Methods: The formulations included an in situ gelling preparation namely Poly(Lactide-co-Glycolide) (PLGA)-in-Triacetin prepared by solution method and Niosomes prepared by lipid layer hydration method. These were evaluated for physicochemical characteristics like size distribution, percentage entrapment, in vitro drug release profiles in phosphate buffered saline (pH 7.4) and stability at different conditions of storage viz; 4°C, room temperature and 37°C. In vivo anti-fertility activity was evaluated in female albino rats showing normal oestrous cycle by giving a single dose of the formulation (5mg/kg body weight) and then checking for number of implantations on 20th day of pregnancy. Also histopathological sections of the uterus and ovary were studied for further evaluation. Results: The formulations showed controlled drug release and enhanced stability whereas in vivo studies showed promising anti-fertility activity for PLGA-in-Triacetin. Conclusions: It was evident from this study, that, PLGA-in-Triacetin was a better candidate for development of a biodegradable, bio-erodable, long acting, injectable contraceptive formulation.

Original languageEnglish
Pages (from-to)233-237
Number of pages5
JournalIndian Journal of Pharmacology
Volume29
Issue number4
Publication statusPublished - 08-1997

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Centchroman
Triacetin
Contraceptive Agents
Fertility
Injections
Polyglactin 910
Liposomes
Uterus
Ovary
Phosphates
Body Weight
Lipids
Pregnancy
Temperature
In Vitro Techniques
polylactic acid-polyglycolic acid copolymer
Drug Liberation

All Science Journal Classification (ASJC) codes

  • Pharmacology

Cite this

Dinesh Shenoy, B. ; Udupa, N. ; Singh, U. V. ; Kumari, Nagaraj. / In-vitro and in-vivo evaluation of novel injectable contraceptive preparations of Centchroman. In: Indian Journal of Pharmacology. 1997 ; Vol. 29, No. 4. pp. 233-237.
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In-vitro and in-vivo evaluation of novel injectable contraceptive preparations of Centchroman. / Dinesh Shenoy, B.; Udupa, N.; Singh, U. V.; Kumari, Nagaraj.

In: Indian Journal of Pharmacology, Vol. 29, No. 4, 08.1997, p. 233-237.

Research output: Contribution to journalArticle

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