In vitro and in vivo hepatoprotective effects of the total alkaloid fraction of Hygrophila auriculata leaves

Vasanth P. Raj, Raghu H. Chandrasekhar, P. Vijayan, S. A. Dhanaraj, Mallikarjuna C. Rao, Venkata J. Rao, K. Nitesh

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Abstract

Objective: To investigate the total alkaloid fraction of the methanol extract of leaves of Hygrophila auriculata for its hepatoprotective activity against CCl4-induced toxicity in freshly isolated rat hepatocytes, HepG2 cells, and animal models. Materials and Methods: Mature leaves of H. auriculata were collected, authenticated, and subjected to methanolic extraction followed by isolation of total alkaloid fraction. Freshly isolated rat hepatocytes were exposed to CCl4 (1%) along with/without various concentrations of the total alkaloid fraction (80-40 μg/ml). Protection of human liver-derived HepG2 cells against CCl4-induced damage was determined by the MTT assay. Twenty-four healthy Wistar albino rats (150-200 g) of either sex were used for the in vivo investigations. Liver damage was induced by administration of 30% CCl4 suspended in olive oil (1 ml/kg body weight, i.p). Results: The antihepatotoxic effect of the total alkaloid fraction was observed in freshly isolated rat hepatocytes at very low concentrations (80-40 μg/ml). A dose-dependent increase in the percentage viability was observed when CCl4-exposed HepG2 cells were treated with different concentrations of the total alkaloid fraction. Its in vivo hepatoprotective effect at 80 mg/kg body weight was comparable with that of the standard Silymarin at 250 mg/kg body weight. Conclusion: The total alkaloid fraction was able to normalize the biochemical levels which were altered due to CCl4 intoxication.

Original languageEnglish
Pages (from-to)99-104
Number of pages6
JournalIndian Journal of Pharmacology
Volume42
Issue number2
DOIs
Publication statusPublished - 01-03-2010

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Acanthaceae
Alkaloids
Hep G2 Cells
Hepatocytes
Body Weight
Silymarin
Liver
Methanol
In Vitro Techniques
Wistar Rats
Animal Models

All Science Journal Classification (ASJC) codes

  • Pharmacology
  • Pharmacology (medical)

Cite this

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abstract = "Objective: To investigate the total alkaloid fraction of the methanol extract of leaves of Hygrophila auriculata for its hepatoprotective activity against CCl4-induced toxicity in freshly isolated rat hepatocytes, HepG2 cells, and animal models. Materials and Methods: Mature leaves of H. auriculata were collected, authenticated, and subjected to methanolic extraction followed by isolation of total alkaloid fraction. Freshly isolated rat hepatocytes were exposed to CCl4 (1{\%}) along with/without various concentrations of the total alkaloid fraction (80-40 μg/ml). Protection of human liver-derived HepG2 cells against CCl4-induced damage was determined by the MTT assay. Twenty-four healthy Wistar albino rats (150-200 g) of either sex were used for the in vivo investigations. Liver damage was induced by administration of 30{\%} CCl4 suspended in olive oil (1 ml/kg body weight, i.p). Results: The antihepatotoxic effect of the total alkaloid fraction was observed in freshly isolated rat hepatocytes at very low concentrations (80-40 μg/ml). A dose-dependent increase in the percentage viability was observed when CCl4-exposed HepG2 cells were treated with different concentrations of the total alkaloid fraction. Its in vivo hepatoprotective effect at 80 mg/kg body weight was comparable with that of the standard Silymarin at 250 mg/kg body weight. Conclusion: The total alkaloid fraction was able to normalize the biochemical levels which were altered due to CCl4 intoxication.",
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In vitro and in vivo hepatoprotective effects of the total alkaloid fraction of Hygrophila auriculata leaves. / Raj, Vasanth P.; Chandrasekhar, Raghu H.; Vijayan, P.; Dhanaraj, S. A.; Rao, Mallikarjuna C.; Rao, Venkata J.; Nitesh, K.

In: Indian Journal of Pharmacology, Vol. 42, No. 2, 01.03.2010, p. 99-104.

Research output: Contribution to journalArticle

TY - JOUR

T1 - In vitro and in vivo hepatoprotective effects of the total alkaloid fraction of Hygrophila auriculata leaves

AU - Raj, Vasanth P.

AU - Chandrasekhar, Raghu H.

AU - Vijayan, P.

AU - Dhanaraj, S. A.

AU - Rao, Mallikarjuna C.

AU - Rao, Venkata J.

AU - Nitesh, K.

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N2 - Objective: To investigate the total alkaloid fraction of the methanol extract of leaves of Hygrophila auriculata for its hepatoprotective activity against CCl4-induced toxicity in freshly isolated rat hepatocytes, HepG2 cells, and animal models. Materials and Methods: Mature leaves of H. auriculata were collected, authenticated, and subjected to methanolic extraction followed by isolation of total alkaloid fraction. Freshly isolated rat hepatocytes were exposed to CCl4 (1%) along with/without various concentrations of the total alkaloid fraction (80-40 μg/ml). Protection of human liver-derived HepG2 cells against CCl4-induced damage was determined by the MTT assay. Twenty-four healthy Wistar albino rats (150-200 g) of either sex were used for the in vivo investigations. Liver damage was induced by administration of 30% CCl4 suspended in olive oil (1 ml/kg body weight, i.p). Results: The antihepatotoxic effect of the total alkaloid fraction was observed in freshly isolated rat hepatocytes at very low concentrations (80-40 μg/ml). A dose-dependent increase in the percentage viability was observed when CCl4-exposed HepG2 cells were treated with different concentrations of the total alkaloid fraction. Its in vivo hepatoprotective effect at 80 mg/kg body weight was comparable with that of the standard Silymarin at 250 mg/kg body weight. Conclusion: The total alkaloid fraction was able to normalize the biochemical levels which were altered due to CCl4 intoxication.

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