Abstract

Extracts from natural products, especially microorganisms, have served as a valuable source of diverse molecules in many drug discovery efforts and led to the discovery of several important drugs. Identification of microbial strains having promising biological activities and purifying the bio-molecules responsible for the activities, have led to the discovery of many bioactive molecules. Extracellular, as well as intracellular, extracts of the metabolites of thirty-six bacterial and twenty-four fungal isolates, grown under unusual conditions such as high temperature, high salt and low sugar concentrations, were in vitro tested for their cytotoxic potential on various cancer cell lines. The extracts were screened on HeLa and MCF-7 cell lines to study the cytotoxic potential. Nuclear staining and flow cytometric studies were carried out to assess the potential of the extracts in arresting the cell cycle. The crude ethylacetate extract of isolate F-21 showed promising results by MTT assay with IC50 as low as 20.37±0.36 μg/mL on HeLa, and 44.75±0.81 μg/mL on MCF-7 cells, comparable with Cisplatin. The isolate F-21 was identified as Aspergillus sp. Promising results were also obtained with B-2C and B-4E strains. Morphological studies, biochemical tests and preliminary chemical investigation of the extracts were also carried out.

Original languageEnglish
Pages (from-to)279-287
Number of pages9
JournalBrazilian Journal of Pharmaceutical Sciences
Volume47
Issue number2
DOIs
Publication statusPublished - 2011

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MCF-7 Cells
Ecosystem
Cell Line
Drug Discovery
Aspergillus
Biological Products
Complex Mixtures
Cisplatin
Inhibitory Concentration 50
Cell Cycle
Salts
Staining and Labeling
Temperature
Pharmaceutical Preparations
Neoplasms
In Vitro Techniques
2-(4-bromo-2,5-dimethoxyphenyl)ethylamine

All Science Journal Classification (ASJC) codes

  • Pharmacology, Toxicology and Pharmaceutics(all)

Cite this

@article{13037686eddd46c5a85fb23ffe02c71c,
title = "In vitro anticancer activity of microbial isolates from diverse habitats",
abstract = "Extracts from natural products, especially microorganisms, have served as a valuable source of diverse molecules in many drug discovery efforts and led to the discovery of several important drugs. Identification of microbial strains having promising biological activities and purifying the bio-molecules responsible for the activities, have led to the discovery of many bioactive molecules. Extracellular, as well as intracellular, extracts of the metabolites of thirty-six bacterial and twenty-four fungal isolates, grown under unusual conditions such as high temperature, high salt and low sugar concentrations, were in vitro tested for their cytotoxic potential on various cancer cell lines. The extracts were screened on HeLa and MCF-7 cell lines to study the cytotoxic potential. Nuclear staining and flow cytometric studies were carried out to assess the potential of the extracts in arresting the cell cycle. The crude ethylacetate extract of isolate F-21 showed promising results by MTT assay with IC50 as low as 20.37±0.36 μg/mL on HeLa, and 44.75±0.81 μg/mL on MCF-7 cells, comparable with Cisplatin. The isolate F-21 was identified as Aspergillus sp. Promising results were also obtained with B-2C and B-4E strains. Morphological studies, biochemical tests and preliminary chemical investigation of the extracts were also carried out.",
author = "Angel Treasathomas and {Venkata Rao}, Josyula and Subrahmanyam, {Volety Mallikarjuna} and Chandrashekhar, {Hariharapura Raghu} and Naseer Maliyakkal and Kisan, {Tukaram Kedar} and Alex Joseph and Nayanabhirama Udupa",
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AU - Venkata Rao, Josyula

AU - Subrahmanyam, Volety Mallikarjuna

AU - Chandrashekhar, Hariharapura Raghu

AU - Maliyakkal, Naseer

AU - Kisan, Tukaram Kedar

AU - Joseph, Alex

AU - Udupa, Nayanabhirama

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