In-vitro antioxidant activity of extracts of Avipattikar churna

U. Kaushik, P. Lachake, C.S. Shreedhara, H.N. Aswatha Ram

Research output: Contribution to journalArticle

Abstract

Free radicals are highly reactive chemical species indicated in various diseases and ailments. In order to study the antioxidant ability of Avipattikar churna, the aqueous and methanolic extracts of Avipattikar churna was evaluated for in vitro antioxidant potential. The free radical scavenging activity was carried out using the reactivity of extracts towards different radicals such as 2,2 - Azino bis (3-ethyl Benzo Thiazole - 6 - Sulphonic acid (ABTS), 1,1-Diphenyl-2- Picryl Hydrazine (DPPH) and 1:10- Phenanthroline hydrate (O-phenenthroline). The superoxide scavenging activity was measured by spectrophotometric method using (Nitroblue tetrazolium) NBT. The total antioxidant potential of aqueous and methanolic extracts of Avipattikar churna was also evaluated.
Original languageEnglish
Pages (from-to)581-589
Number of pages9
JournalPharmacologyonline
Volume3
Publication statusPublished - 2009

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hydrazine
Antioxidants
Free Radicals
Nitroblue Tetrazolium
Thiazoles
Sulfonic Acids
Superoxides
In Vitro Techniques
diphenyl
2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid
1,10-phenanthroline

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Kaushik, U. ; Lachake, P. ; Shreedhara, C.S. ; Aswatha Ram, H.N. / In-vitro antioxidant activity of extracts of Avipattikar churna. In: Pharmacologyonline. 2009 ; Vol. 3. pp. 581-589.
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abstract = "Free radicals are highly reactive chemical species indicated in various diseases and ailments. In order to study the antioxidant ability of Avipattikar churna, the aqueous and methanolic extracts of Avipattikar churna was evaluated for in vitro antioxidant potential. The free radical scavenging activity was carried out using the reactivity of extracts towards different radicals such as 2,2 - Azino bis (3-ethyl Benzo Thiazole - 6 - Sulphonic acid (ABTS), 1,1-Diphenyl-2- Picryl Hydrazine (DPPH) and 1:10- Phenanthroline hydrate (O-phenenthroline). The superoxide scavenging activity was measured by spectrophotometric method using (Nitroblue tetrazolium) NBT. The total antioxidant potential of aqueous and methanolic extracts of Avipattikar churna was also evaluated.",
author = "U. Kaushik and P. Lachake and C.S. Shreedhara and {Aswatha Ram}, H.N.",
note = "Export Date: 10 November 2017 Correspondence Address: Aswatha Ram, H. N.; Department of Pharmacognosy, Manipal College of Pharmaceutical Sciences, Manipal University, Manipal, India; email: aswatharam@gmail.com Chemicals/CAS: 1,1 diphenyl 2 picrylhydrazyl, 1898-66-4; 1,10 phenanthroline, 3829-86-5, 66-71-7; 2,2' azinobis(3 ethylbenzothiazoline 6 sulfonic acid), 28752-68-3; nitroblue tetrazolium, 298-83-9 References: Formulary of Ayurvedic medicine, , 2nd revised English edition. Anonymous; Gutteridge, J.M., Lipid peroxidation and antioxidants as biomarkers of tissue damage (1995) Clin. Chem, 41, p. 1819; Shri rajeshwaradatta shastri, Bhaisajyaratnavali of Shri Govinddas, 18th edition, 922; Re, R., Pellegrini, N., Proteggente, A., Pannala, A., Yang, M., Rice-Evans, C., Antioxidant activity applying an improved ABTS radical cation assay (1999) Free Rad. Biol. Med, 26, p. 1231; Sreejayan, N., Rao, M.N.A., Free radical scavenging activity of curcuminoids (1996) Drug Res, 46, p. 169; Govindarajan, R., Rastogi, S., Vijay Kumar, M., Shirwaikar, A., Rawat, A.S., Mehrotra, S., Pushpagandhan, P., Studies on the antioxidant activity of Desmodium gangetieum (2003) Biol. Pharm. Bull, 26 (10), pp. 1424-1427; Prieto, P., Pinedo, M., Aguilar, M., (1999) Biochem, 269, pp. 337-341; Shirwaikar, A., Prabhu, K.S., Punitha, I.S.R., In-vitro antioxidant studies of Sphaeranthus indicus (Linn) (2006) Indian J. Exp. Biol, 44, p. 993; Halliwell, B., Gutteridge, J.M., Free radicals in Biology and medicine, pp. 23-30. , Clarendon press, Oxford, pp; Glucin, I., Buyukokuroglu, M.E., Oktay, M., On the in vitro antioxidant properties of melatonin (2002) J. Pineal Res, 33, pp. 167-171; Glucin, I., Oktay, M., Kufrevioglu, O.L., Determination of antioxidant activity of lichen Citraria islandica (L) (2002) Ach. J. Ethnopharmacol, 79, pp. 325-329; Valco, M., Leibfritz, D., Monocol, J., Free radical and antioxidants in normal physiological functions and human disease (2007) The Int. J. Biochem and Cell Biology, 39, pp. 44-48; Aruoma, O.I., Free radicals, oxidative stress and antioxidants in human health and disease (1998) Journal of the Americal Oil Chemista Society, 75, pp. 199-212; Stanner, S.A., Hughes, J., Kelly, C.N., Review of the epidemiological evidence for the 'antioxidant hypothesis' (2004) Public Health Nutrition, 7, pp. 407-422; Branen, A.L., Toxciology and biochemistry of butylated hydroxyl anisole and butylated hydroxyl toluene (1995) Journal of the American Oil Chemists Society, 52, pp. 59-63; Ito, N., Fukushima, S., Hassegawa, A., carcinogenicity of butylated hydroxyl anisole in F344 rats (1983) Journal of National Cancer Institute, 70, pp. 343-347; Marx, J.L., Oxygen free radicals linked to many diseases (1987) Science, 235, p. 529; Covacci, V., Torsello, A., Palozza, P., Sgambato, A., Ramano, G., Boninsegna, A., Cittadini, A., Wolf F.I (2001) Chem. Res. Toxicol, 14, p. 1492; Nakatani, N., Inatani, R., Ohta, H., Nishioka, A., Chemical constituents of peppers (Piper Spp) and application to food preservation: Naturally occurring antioxidant compound (1986) Environ. Health Perspect, 67, p. 135; Agarwal, A.K., Rao, C.V., Sairam, K., Joshi, V.K., Goel, R.K., Effect of Piper longum, Zingiber officinale and Ferula species on gastric ulceration and secretion in rats (2000) Indian J. Exp. Biol, 38, p. 994",
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Kaushik, U, Lachake, P, Shreedhara, CS & Aswatha Ram, HN 2009, 'In-vitro antioxidant activity of extracts of Avipattikar churna', Pharmacologyonline, vol. 3, pp. 581-589.

In-vitro antioxidant activity of extracts of Avipattikar churna. / Kaushik, U.; Lachake, P.; Shreedhara, C.S.; Aswatha Ram, H.N.

In: Pharmacologyonline, Vol. 3, 2009, p. 581-589.

Research output: Contribution to journalArticle

TY - JOUR

T1 - In-vitro antioxidant activity of extracts of Avipattikar churna

AU - Kaushik, U.

AU - Lachake, P.

AU - Shreedhara, C.S.

AU - Aswatha Ram, H.N.

N1 - Export Date: 10 November 2017 Correspondence Address: Aswatha Ram, H. N.; Department of Pharmacognosy, Manipal College of Pharmaceutical Sciences, Manipal University, Manipal, India; email: aswatharam@gmail.com Chemicals/CAS: 1,1 diphenyl 2 picrylhydrazyl, 1898-66-4; 1,10 phenanthroline, 3829-86-5, 66-71-7; 2,2' azinobis(3 ethylbenzothiazoline 6 sulfonic acid), 28752-68-3; nitroblue tetrazolium, 298-83-9 References: Formulary of Ayurvedic medicine, , 2nd revised English edition. Anonymous; Gutteridge, J.M., Lipid peroxidation and antioxidants as biomarkers of tissue damage (1995) Clin. Chem, 41, p. 1819; Shri rajeshwaradatta shastri, Bhaisajyaratnavali of Shri Govinddas, 18th edition, 922; Re, R., Pellegrini, N., Proteggente, A., Pannala, A., Yang, M., Rice-Evans, C., Antioxidant activity applying an improved ABTS radical cation assay (1999) Free Rad. Biol. Med, 26, p. 1231; Sreejayan, N., Rao, M.N.A., Free radical scavenging activity of curcuminoids (1996) Drug Res, 46, p. 169; Govindarajan, R., Rastogi, S., Vijay Kumar, M., Shirwaikar, A., Rawat, A.S., Mehrotra, S., Pushpagandhan, P., Studies on the antioxidant activity of Desmodium gangetieum (2003) Biol. Pharm. Bull, 26 (10), pp. 1424-1427; Prieto, P., Pinedo, M., Aguilar, M., (1999) Biochem, 269, pp. 337-341; Shirwaikar, A., Prabhu, K.S., Punitha, I.S.R., In-vitro antioxidant studies of Sphaeranthus indicus (Linn) (2006) Indian J. Exp. Biol, 44, p. 993; Halliwell, B., Gutteridge, J.M., Free radicals in Biology and medicine, pp. 23-30. , Clarendon press, Oxford, pp; Glucin, I., Buyukokuroglu, M.E., Oktay, M., On the in vitro antioxidant properties of melatonin (2002) J. Pineal Res, 33, pp. 167-171; Glucin, I., Oktay, M., Kufrevioglu, O.L., Determination of antioxidant activity of lichen Citraria islandica (L) (2002) Ach. J. Ethnopharmacol, 79, pp. 325-329; Valco, M., Leibfritz, D., Monocol, J., Free radical and antioxidants in normal physiological functions and human disease (2007) The Int. J. Biochem and Cell Biology, 39, pp. 44-48; Aruoma, O.I., Free radicals, oxidative stress and antioxidants in human health and disease (1998) Journal of the Americal Oil Chemista Society, 75, pp. 199-212; Stanner, S.A., Hughes, J., Kelly, C.N., Review of the epidemiological evidence for the 'antioxidant hypothesis' (2004) Public Health Nutrition, 7, pp. 407-422; Branen, A.L., Toxciology and biochemistry of butylated hydroxyl anisole and butylated hydroxyl toluene (1995) Journal of the American Oil Chemists Society, 52, pp. 59-63; Ito, N., Fukushima, S., Hassegawa, A., carcinogenicity of butylated hydroxyl anisole in F344 rats (1983) Journal of National Cancer Institute, 70, pp. 343-347; Marx, J.L., Oxygen free radicals linked to many diseases (1987) Science, 235, p. 529; Covacci, V., Torsello, A., Palozza, P., Sgambato, A., Ramano, G., Boninsegna, A., Cittadini, A., Wolf F.I (2001) Chem. Res. Toxicol, 14, p. 1492; Nakatani, N., Inatani, R., Ohta, H., Nishioka, A., Chemical constituents of peppers (Piper Spp) and application to food preservation: Naturally occurring antioxidant compound (1986) Environ. Health Perspect, 67, p. 135; Agarwal, A.K., Rao, C.V., Sairam, K., Joshi, V.K., Goel, R.K., Effect of Piper longum, Zingiber officinale and Ferula species on gastric ulceration and secretion in rats (2000) Indian J. Exp. Biol, 38, p. 994

PY - 2009

Y1 - 2009

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AB - Free radicals are highly reactive chemical species indicated in various diseases and ailments. In order to study the antioxidant ability of Avipattikar churna, the aqueous and methanolic extracts of Avipattikar churna was evaluated for in vitro antioxidant potential. The free radical scavenging activity was carried out using the reactivity of extracts towards different radicals such as 2,2 - Azino bis (3-ethyl Benzo Thiazole - 6 - Sulphonic acid (ABTS), 1,1-Diphenyl-2- Picryl Hydrazine (DPPH) and 1:10- Phenanthroline hydrate (O-phenenthroline). The superoxide scavenging activity was measured by spectrophotometric method using (Nitroblue tetrazolium) NBT. The total antioxidant potential of aqueous and methanolic extracts of Avipattikar churna was also evaluated.

M3 - Article

VL - 3

SP - 581

EP - 589

JO - Pharmacologyonline

JF - Pharmacologyonline

SN - 1827-8620

ER -