In vitro antioxidant studies of Annona squamosa Linn. Leaves

A. Shirwaikar, K. Rajendran, C.D. Kumar

Research output: Contribution to journalArticle

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Abstract

The free radical scavenging potential of the leaves of A. squamosa was studied by using different antioxidant models of screening. The ethanolic extract at 1000 μg/ ml showed maximum scavenging of the radical cation, 2,2- azinobis- (3-ethylbenzothiazoline- 6- sulphonate) (ABTS) observed upto 99.07% followed by the scavenging of the stable radical 1,1-diphenyl, 2- picryl hydrazyl (DPPH) (89.77%) and nitric oxide radical (73.64%) at the same concentration. However, the extract showed only moderate scavenging activity of superoxide radicals and antilipid peroxidation potential, which was performed using rat- brain homogenate. The findings justify the therapeutic applications of the plant in the indigenous system of medicine, augmenting its therapeutic value.
Original languageEnglish
Pages (from-to)803-807
Number of pages5
JournalIndian Journal of Experimental Biology
Volume42
Issue number8
Publication statusPublished - 2004

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Annona
Antioxidants
Traditional Medicine
Systems Analysis
Superoxides
Free Radicals
Cations
Nitric Oxide
Brain
Therapeutics
In Vitro Techniques

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Shirwaikar, A., Rajendran, K., & Kumar, C. D. (2004). In vitro antioxidant studies of Annona squamosa Linn. Leaves. Indian Journal of Experimental Biology, 42(8), 803-807.
Shirwaikar, A. ; Rajendran, K. ; Kumar, C.D. / In vitro antioxidant studies of Annona squamosa Linn. Leaves. In: Indian Journal of Experimental Biology. 2004 ; Vol. 42, No. 8. pp. 803-807.
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abstract = "The free radical scavenging potential of the leaves of A. squamosa was studied by using different antioxidant models of screening. The ethanolic extract at 1000 μg/ ml showed maximum scavenging of the radical cation, 2,2- azinobis- (3-ethylbenzothiazoline- 6- sulphonate) (ABTS) observed upto 99.07{\%} followed by the scavenging of the stable radical 1,1-diphenyl, 2- picryl hydrazyl (DPPH) (89.77{\%}) and nitric oxide radical (73.64{\%}) at the same concentration. However, the extract showed only moderate scavenging activity of superoxide radicals and antilipid peroxidation potential, which was performed using rat- brain homogenate. The findings justify the therapeutic applications of the plant in the indigenous system of medicine, augmenting its therapeutic value.",
author = "A. Shirwaikar and K. Rajendran and C.D. Kumar",
note = "Cited By :90 Export Date: 10 November 2017 CODEN: IJEBA Correspondence Address: Shirwaikar, A.; Department of Pharmacognosy, College of Pharmaceutical Sciences, Manipal 576 104, India; email: annieshirwaikar@yahoo.com Chemicals/CAS: Antioxidants; Free Radical Scavengers; Plant Extracts References: Cross, C.E., Oxygen radicals and human disease (1987) Annal Int Med, 107, p. 526; Marx, J.L., Oxygen free radicals linked to many diseases (1987) Science, 235, p. 529; Arora, A., Sairam, R.K., Srivastava, G.C., Oxidative stress and antioxidative system in plants (2002) Curr Sci, 82, p. 1227; Geesin, J.G., Gordon, J.S., Berg, R.A., Retinoids affect collagen synthesis through inhibition of ascorbate- Induced lipid peroxidation in cultured human dermal fibroblasts (1990) Arch Biochem Biophys, 278, p. 352; Warrier, P.K., Nambiar, V.P.K., Raman Kutty, C., (1996) Indian Medicinal Plants, 1, p. 160. , Orient Longman, Hyderabad, India; Yoganarasimhan, S.N., (2000) Medicinal Plants of India- Tamil Nadu, 2, p. 48. , Cyber Media, Bangalore, India; Nadkarni, A.K., (2000) Indian Materia Medica, 1, p. 116. , Popular Prakashan Ltd., Mumbai, India; Atique, A., Iqbql, M., Ghouse, A.K.M., Use of Annona squamosa and Pipier nigrum against diabetes (1985) Fitoterapia, 56, p. 190; Topno, K.K., Plants used by tribals of Chotanagpur against diabetes (1997) Botanica, 47, p. 99; Auddy, B., Ferreira, M., Blasina, F., Lafon, L., Arredondo, F., Dajas, F., Tripathi, P.C., Mukherjee, B., Screening of antioxidant activity of three Indian medicinal plants, traditionally used for the management of neurodegenerative diseases (2003) J Ethnopharmacol, 84, p. 132; Ohkawa, H., Ohishi, N., Yagi, K., Assay for lipid peroxides in animal tissues by thio barbituric acid reaction (1979) Anal Biochem, 95, p. 351; Prashanth Kumar, V., Shashidhara, S., Kumar, M.M., Sridhara, B.Y., Effect of Luffa echinata on lipid peroxidation and free radical scavenging activity (2000) J Pharm Pharmacol, 52, p. 891; Re, R., Pellegrini, N., Proteggente, A., Pannala, A., Yang, M., Rice- Evans, C., Antioxidant activity applying an improved ABTS radical cation assay (1999) Free Rad Biol Med, 26, p. 1231; Sreejayan, N., Rao, M.N.A., Free radical scavenging activity of curcuminoids (1996) Drug Res, 46, p. 169; Sreejayan, N., Rao, M.N.A., Nitric oxide scavenging by curcuminoids (1997) J Pharm Pharmacol, 49, p. 105; Shirwaikar, A., Somashekar, A.P., Antiinflammatory activity and free radical scavenging studies of Aristolochia bracteolata Lam (2003) Indian J Pharm Sci, 65, p. 68; Green, L.C., Wagner, D.A., Glogowski, J., Skipper, P.L., Wishnok, J.S., Tannenbaum, S.R., Analysis of nitrate and 15N in biological fluids (1982) Anal Biochem, 126, p. 131; Marcocci, L., Maguire, J.J., Droy- Lefaix, M.T., Packer, L., The nitric oxide scavenging property of Ginko biloba extract EGB 761 (1994) Biochem Biophys Res Commun, 201, p. 748; Sanchez- Moreno, C., Methods used to evaluate the free radical scavenging activity in foods and biological systems (2002) Food Sci Tech Int, 8, p. 122; Henry, L.E.A., Halliwell, B., Hall, D.O., The superoxide dismutase activity of various photosynthetic organisms measured by a new and a rapid assay technique (1976) FEBS Lett, 66, p. 303; Youdim, K.A., Joseph, J.A., A possible emerging role of phytochemicals in improving age-related neurological dysfunctions: A multiplicity of effects (2001) Free Rod Biol Med, 30, p. 583; Gutteridge, J.M.C., Age pigments and free radicals: Fluorescent lipid complexes formed by iron and copper containing proteins (1985) Biochim Biophys Acta, 834, p. 144; Halliwell, B., Superoxide- Dependent formation of hydroxy free radicals in the presence of iron chelates (1978) FEBS Lett, 92, p. 321; Braugghler, J.M., Duncan, C.A., Chase, L.R., The involvement of iron in lipid peroxidation. Importance of ferrous to ferric ratio in initiation (1986) J Biol Chem, 261, p. 10282; Record, I.R., Dreosti, I.E., Mc Inerney, J.K., Changes in plasma antioxidant status following consumption of diets high or low in fruits and vegetables or following dietary supplementation with an antioxidant mixture (2000) Br J Nutr, 85, p. 459; Pryor, W.A., Oxy- Radicals and related species, their formation, lifetimes and reactions (1986) Annu Rev Physiol, 48, p. 657; Rice- Evans, C., Miller, N.J., Factors influencing the antioxidant activity determined by the ABTS .+ radical cation assay (1997) Free Rad Res, 26, p. 195; Ialenti, A., Moncada, S., Di Rosa, M., Modulation of adjuvant arthritis by endogenous nitric oxide (1993) Br J Pharmacol, 110, p. 701; Ross, R., The pathogenesis of atherosclerosis: A perspective for the 1990s (1993) Nature, 362, p. 801; Marcocci, L., Packer, L., Droy- Lefaiz, M.T., Sekaki, A., Gardes- Albert, M., Antioxidant action of Ginko biloba extract Egb 761 (1994) Meth Enzymol, 234, p. 462; Blois, M.S., Antioxidant determinations by the use of stable free radical (1958) Nature, 26, p. 1199; Kamalakkannan, N., Stanley Mainzen Prince, P., Effect of Aegle marmelos fruit extract on tissue antioxidants in streptozotocin diabetic rats (2003) Indian J Exp Biol, 41, p. 1288; Gibanananda, R., Akhtar, H.S., Oxidents, antioxidants and carcinogenesis (2002) Indian J Exp Biol, 40, p. 1214; Perrisoud, D., Testa, B., Hepatic Pharmacology: Mechanisms of action and classification of antinecrotic hepatoprotective agents (1982) Trends Pharmacol Sci, 3, p. 365; Glyglewski, R.J., Korbut, R., Robak, J., Swies, J., On the mechanisms of antithrombotic action of flavonoids (1987) Biochem Pharmacol, 36, p. 317; Corvazier, E., Maclouf, J., Interference of some flavonoids and non- Steroidal antiinflammatory drugs with oxidative metabolism of arachidonic acid by human platelets and neutrophils (1985) Biochim Biophys Acta, 835, p. 315; Middleton, E., The flavonoids (1984) Trends Pharmacol Sci, 5, p. 335; Robak, J., Glyglewski, R.J., Flavonoids are scavengers of superoxide anions (1988) Biochem Pharmacol, 37, p. 837; Ratty, A.K., Sunamoto, J., Das, N.P., Interaction of flavonoids with 1,1,- Diphenyl-2-picryl hydrazyl free radical, liposomal membranes and soybean lipoxygenase-1 (1998) Biochem Pharmacol, 37, p. 989",
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Shirwaikar, A, Rajendran, K & Kumar, CD 2004, 'In vitro antioxidant studies of Annona squamosa Linn. Leaves', Indian Journal of Experimental Biology, vol. 42, no. 8, pp. 803-807.

In vitro antioxidant studies of Annona squamosa Linn. Leaves. / Shirwaikar, A.; Rajendran, K.; Kumar, C.D.

In: Indian Journal of Experimental Biology, Vol. 42, No. 8, 2004, p. 803-807.

Research output: Contribution to journalArticle

TY - JOUR

T1 - In vitro antioxidant studies of Annona squamosa Linn. Leaves

AU - Shirwaikar, A.

AU - Rajendran, K.

AU - Kumar, C.D.

N1 - Cited By :90 Export Date: 10 November 2017 CODEN: IJEBA Correspondence Address: Shirwaikar, A.; Department of Pharmacognosy, College of Pharmaceutical Sciences, Manipal 576 104, India; email: annieshirwaikar@yahoo.com Chemicals/CAS: Antioxidants; Free Radical Scavengers; Plant Extracts References: Cross, C.E., Oxygen radicals and human disease (1987) Annal Int Med, 107, p. 526; Marx, J.L., Oxygen free radicals linked to many diseases (1987) Science, 235, p. 529; Arora, A., Sairam, R.K., Srivastava, G.C., Oxidative stress and antioxidative system in plants (2002) Curr Sci, 82, p. 1227; Geesin, J.G., Gordon, J.S., Berg, R.A., Retinoids affect collagen synthesis through inhibition of ascorbate- Induced lipid peroxidation in cultured human dermal fibroblasts (1990) Arch Biochem Biophys, 278, p. 352; Warrier, P.K., Nambiar, V.P.K., Raman Kutty, C., (1996) Indian Medicinal Plants, 1, p. 160. , Orient Longman, Hyderabad, India; Yoganarasimhan, S.N., (2000) Medicinal Plants of India- Tamil Nadu, 2, p. 48. , Cyber Media, Bangalore, India; Nadkarni, A.K., (2000) Indian Materia Medica, 1, p. 116. , Popular Prakashan Ltd., Mumbai, India; Atique, A., Iqbql, M., Ghouse, A.K.M., Use of Annona squamosa and Pipier nigrum against diabetes (1985) Fitoterapia, 56, p. 190; Topno, K.K., Plants used by tribals of Chotanagpur against diabetes (1997) Botanica, 47, p. 99; Auddy, B., Ferreira, M., Blasina, F., Lafon, L., Arredondo, F., Dajas, F., Tripathi, P.C., Mukherjee, B., Screening of antioxidant activity of three Indian medicinal plants, traditionally used for the management of neurodegenerative diseases (2003) J Ethnopharmacol, 84, p. 132; Ohkawa, H., Ohishi, N., Yagi, K., Assay for lipid peroxides in animal tissues by thio barbituric acid reaction (1979) Anal Biochem, 95, p. 351; Prashanth Kumar, V., Shashidhara, S., Kumar, M.M., Sridhara, B.Y., Effect of Luffa echinata on lipid peroxidation and free radical scavenging activity (2000) J Pharm Pharmacol, 52, p. 891; Re, R., Pellegrini, N., Proteggente, A., Pannala, A., Yang, M., Rice- Evans, C., Antioxidant activity applying an improved ABTS radical cation assay (1999) Free Rad Biol Med, 26, p. 1231; Sreejayan, N., Rao, M.N.A., Free radical scavenging activity of curcuminoids (1996) Drug Res, 46, p. 169; Sreejayan, N., Rao, M.N.A., Nitric oxide scavenging by curcuminoids (1997) J Pharm Pharmacol, 49, p. 105; Shirwaikar, A., Somashekar, A.P., Antiinflammatory activity and free radical scavenging studies of Aristolochia bracteolata Lam (2003) Indian J Pharm Sci, 65, p. 68; Green, L.C., Wagner, D.A., Glogowski, J., Skipper, P.L., Wishnok, J.S., Tannenbaum, S.R., Analysis of nitrate and 15N in biological fluids (1982) Anal Biochem, 126, p. 131; Marcocci, L., Maguire, J.J., Droy- Lefaix, M.T., Packer, L., The nitric oxide scavenging property of Ginko biloba extract EGB 761 (1994) Biochem Biophys Res Commun, 201, p. 748; Sanchez- Moreno, C., Methods used to evaluate the free radical scavenging activity in foods and biological systems (2002) Food Sci Tech Int, 8, p. 122; Henry, L.E.A., Halliwell, B., Hall, D.O., The superoxide dismutase activity of various photosynthetic organisms measured by a new and a rapid assay technique (1976) FEBS Lett, 66, p. 303; Youdim, K.A., Joseph, J.A., A possible emerging role of phytochemicals in improving age-related neurological dysfunctions: A multiplicity of effects (2001) Free Rod Biol Med, 30, p. 583; Gutteridge, J.M.C., Age pigments and free radicals: Fluorescent lipid complexes formed by iron and copper containing proteins (1985) Biochim Biophys Acta, 834, p. 144; Halliwell, B., Superoxide- Dependent formation of hydroxy free radicals in the presence of iron chelates (1978) FEBS Lett, 92, p. 321; Braugghler, J.M., Duncan, C.A., Chase, L.R., The involvement of iron in lipid peroxidation. Importance of ferrous to ferric ratio in initiation (1986) J Biol Chem, 261, p. 10282; Record, I.R., Dreosti, I.E., Mc Inerney, J.K., Changes in plasma antioxidant status following consumption of diets high or low in fruits and vegetables or following dietary supplementation with an antioxidant mixture (2000) Br J Nutr, 85, p. 459; Pryor, W.A., Oxy- Radicals and related species, their formation, lifetimes and reactions (1986) Annu Rev Physiol, 48, p. 657; Rice- Evans, C., Miller, N.J., Factors influencing the antioxidant activity determined by the ABTS .+ radical cation assay (1997) Free Rad Res, 26, p. 195; Ialenti, A., Moncada, S., Di Rosa, M., Modulation of adjuvant arthritis by endogenous nitric oxide (1993) Br J Pharmacol, 110, p. 701; Ross, R., The pathogenesis of atherosclerosis: A perspective for the 1990s (1993) Nature, 362, p. 801; Marcocci, L., Packer, L., Droy- Lefaiz, M.T., Sekaki, A., Gardes- Albert, M., Antioxidant action of Ginko biloba extract Egb 761 (1994) Meth Enzymol, 234, p. 462; Blois, M.S., Antioxidant determinations by the use of stable free radical (1958) Nature, 26, p. 1199; Kamalakkannan, N., Stanley Mainzen Prince, P., Effect of Aegle marmelos fruit extract on tissue antioxidants in streptozotocin diabetic rats (2003) Indian J Exp Biol, 41, p. 1288; Gibanananda, R., Akhtar, H.S., Oxidents, antioxidants and carcinogenesis (2002) Indian J Exp Biol, 40, p. 1214; Perrisoud, D., Testa, B., Hepatic Pharmacology: Mechanisms of action and classification of antinecrotic hepatoprotective agents (1982) Trends Pharmacol Sci, 3, p. 365; Glyglewski, R.J., Korbut, R., Robak, J., Swies, J., On the mechanisms of antithrombotic action of flavonoids (1987) Biochem Pharmacol, 36, p. 317; Corvazier, E., Maclouf, J., Interference of some flavonoids and non- Steroidal antiinflammatory drugs with oxidative metabolism of arachidonic acid by human platelets and neutrophils (1985) Biochim Biophys Acta, 835, p. 315; Middleton, E., The flavonoids (1984) Trends Pharmacol Sci, 5, p. 335; Robak, J., Glyglewski, R.J., Flavonoids are scavengers of superoxide anions (1988) Biochem Pharmacol, 37, p. 837; Ratty, A.K., Sunamoto, J., Das, N.P., Interaction of flavonoids with 1,1,- Diphenyl-2-picryl hydrazyl free radical, liposomal membranes and soybean lipoxygenase-1 (1998) Biochem Pharmacol, 37, p. 989

PY - 2004

Y1 - 2004

N2 - The free radical scavenging potential of the leaves of A. squamosa was studied by using different antioxidant models of screening. The ethanolic extract at 1000 μg/ ml showed maximum scavenging of the radical cation, 2,2- azinobis- (3-ethylbenzothiazoline- 6- sulphonate) (ABTS) observed upto 99.07% followed by the scavenging of the stable radical 1,1-diphenyl, 2- picryl hydrazyl (DPPH) (89.77%) and nitric oxide radical (73.64%) at the same concentration. However, the extract showed only moderate scavenging activity of superoxide radicals and antilipid peroxidation potential, which was performed using rat- brain homogenate. The findings justify the therapeutic applications of the plant in the indigenous system of medicine, augmenting its therapeutic value.

AB - The free radical scavenging potential of the leaves of A. squamosa was studied by using different antioxidant models of screening. The ethanolic extract at 1000 μg/ ml showed maximum scavenging of the radical cation, 2,2- azinobis- (3-ethylbenzothiazoline- 6- sulphonate) (ABTS) observed upto 99.07% followed by the scavenging of the stable radical 1,1-diphenyl, 2- picryl hydrazyl (DPPH) (89.77%) and nitric oxide radical (73.64%) at the same concentration. However, the extract showed only moderate scavenging activity of superoxide radicals and antilipid peroxidation potential, which was performed using rat- brain homogenate. The findings justify the therapeutic applications of the plant in the indigenous system of medicine, augmenting its therapeutic value.

M3 - Article

VL - 42

SP - 803

EP - 807

JO - Journal of scientific & industrial research. C. Biological sciences

JF - Journal of scientific & industrial research. C. Biological sciences

SN - 0019-5189

IS - 8

ER -