In vitro antioxidant studies of Sphaeranthus indicus (Linn)

A. Shirwaikar, K.S. Prabhu, I.S.R. Punitha

Research output: Contribution to journalArticle

72 Citations (Scopus)

Abstract

The free radical scavenging potential of the plant S.indicus was studied by using different antioxidant models of screening. The ethanolic extract at 1000 μg/ml showed maximum scavenging of the radical cation, 2,2-azinobis-(3- ethylbenzothiazoline-6-sulphonate) (ABTS) observed upto 41.99 % followed by the scavenging of the stable radical 1,1-diphenyl, 2-picryl hydrazyl (DPPH) (33.27 %), superoxide dismutase (25.14 %) and nitric oxide radical (22.36 %) at the same concentration. However, the extract showed only moderate scavenging activity of iron chelation (14.2 %). Total antioxidant capacity of the extract was found to be 160.85 nmol/g ascorbic acid. The results justify the therapeutic applications of the plant in the indigenous system of medicine, augmenting its therapeutic value.
Original languageEnglish
Pages (from-to)993-996
Number of pages4
JournalIndian Journal of Experimental Biology
Volume44
Issue number12
Publication statusPublished - 2006
Externally publishedYes

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Asteraceae
Antioxidants
Traditional Medicine
Systems Analysis
Ascorbic Acid
Superoxide Dismutase
Free Radicals
Cations
Nitric Oxide
Iron
Therapeutics
In Vitro Techniques
diphenyl
2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid

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Shirwaikar, A., Prabhu, K. S., & Punitha, I. S. R. (2006). In vitro antioxidant studies of Sphaeranthus indicus (Linn). Indian Journal of Experimental Biology, 44(12), 993-996.
Shirwaikar, A. ; Prabhu, K.S. ; Punitha, I.S.R. / In vitro antioxidant studies of Sphaeranthus indicus (Linn). In: Indian Journal of Experimental Biology. 2006 ; Vol. 44, No. 12. pp. 993-996.
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abstract = "The free radical scavenging potential of the plant S.indicus was studied by using different antioxidant models of screening. The ethanolic extract at 1000 μg/ml showed maximum scavenging of the radical cation, 2,2-azinobis-(3- ethylbenzothiazoline-6-sulphonate) (ABTS) observed upto 41.99 {\%} followed by the scavenging of the stable radical 1,1-diphenyl, 2-picryl hydrazyl (DPPH) (33.27 {\%}), superoxide dismutase (25.14 {\%}) and nitric oxide radical (22.36 {\%}) at the same concentration. However, the extract showed only moderate scavenging activity of iron chelation (14.2 {\%}). Total antioxidant capacity of the extract was found to be 160.85 nmol/g ascorbic acid. The results justify the therapeutic applications of the plant in the indigenous system of medicine, augmenting its therapeutic value.",
author = "A. Shirwaikar and K.S. Prabhu and I.S.R. Punitha",
note = "Cited By :62 Export Date: 10 November 2017 CODEN: IJEBA Correspondence Address: Shirwaikar, A.; Department of Pharmacognosy, Manipal College of Pharmaceutical Sciences, Manipal 576 104, India; email: annieshirwaikar@yahoo.com Chemicals/CAS: nitric oxide, 10102-43-9; superoxide dismutase, 37294-21-6, 9016-01-7, 9054-89-1; superoxide, 11062-77-4; Antioxidants; Nitric Oxide, 10102-43-9; Plant Extracts; Superoxide Dismutase, EC 1.15.1.1; Superoxides, 11062-77-4 References: Cross, C.E., Oxygen radicals and human disease (1987) Annal Int Med, 107, p. 526; Marx, J.L., Oxygen free radicals linked to many diseases (1987) Science, 235, p. 529; Ajay Arora, S.R.K., Srivastava, G.C., Oxidative stress and antioxidative system in plants (2002) Curr Sci, 82, p. 1227; Geesin, J.G., Gordon, J.S., Berg, R.A., Retinoids affect collagen synthesis through inhibition of ascorbate- induced lipid peroxidation in cultured human dermal fibroblasts (1990) Arch Biochem Biophys, 278, p. 352; Warrier, P.K., Nambiar, V.P.K., Raman Kutty, C., (2004) Indian Medicinal Plants, 1, p. 180. , Orient Longman, Hyderabad, India; Kokate, C.K., (1986) Practical pharmacognosy, p. 15. , 1st ed, Vallabh Prakashan, New Delhi, India; Yoganarasimhan, S.N., (2000) Medicinal plants of India-Tamil Nadu, 2, p. 512. , Cyber Media, Bangalore, India; Nadkarni, A.K., (2000) Indian materia medica, 1, p. 113. , Popular Prakashan Ltd, Mumbai, India; Shirwaikar Annie, K.R., Dinesh, K., In vitro antioxidant studies of Annona Squamosa (2004) Indian J Exp Biol, 142, p. 803; Sreejayan, N., Rao, M.N.A., Free radical scavenging activity of curcuminoids (1996) Drug Res, 46, p. 169; Sanchez-Moreno, C., Methods used to evaluate the free radical scavenging activity in foods and biological systems (2002) Food Sci Tech Int, 8, p. 122; Henry, L.E.A., Halliwell, B., Hall, D.O., The superoxide dismutase activity of various photosynthetic organisms measured by a new and a rapid assay technique (1976) FEBS Lett, 66, p. 303; Sreejayan, N., Rao, M.N.A., Nitric oxide scavenging by curcuminoids (1997) J Pharm Pharmacol, 49, p. 105; Annie, S., Somashekar, A.P., Antiinflammatory activity and free radical scavenging studies of Aristolochia bracteolata Lam (2003) Indian J Pharm Sci, 65, p. 68; Green, L.C., Wagner, D.A., Glogowski, J., Skipper, P.L., Wishnok, J.S., Tannenbaum, S.R., Analysis of nitrate and 15N in biological fluids (1982) Anal Biochem, 126, p. 131; Marcocci, L., Maguire, J.J., Droy- Lefaix, M.T., Packer, L., The nitric oxide scavenging property of Ginko biloba extract EGB 761 (1994) Biochem Biophys Res Commun, 201, p. 748; Benzie, I.F.F., Strain, J.T., The ferric reducing ability of pLASMA (FRAP) as a measure of {"}antioxidant power{"}: The FRAP assay (1996) Anal Biochem, 239, p. 70; Benzie, I.F.F., Szeto, Y.T., Total antioxidant capacity of Teas by the Ferric Reducing/ antioxidant power assay (1999) J Agric Food Chem, 47, p. 633; Annie, S., Govindrajan, R., Rastogi, S., Vijaykumar, M., Rawat, A.K.S., ehlotra, S.M., Pushpangadan, P., Studies on the Antioxidant activities of Desmodium gangeticum (2003) Biol Pharm Bull, 26, p. 1424; Youdim, K.A., Joseph, J.A., A possible emerging role of phytochemicals in improving age-related neurological dysfunctions: A multiplicity of effects (2001) Free Rad Biol Med, 30, p. 583; Rice- Evans, C., Miller, N.J., Factors influencing the antioxidant activity determined by the ABTS.+ radical cation assay (1997) Free Rad Res, 26, p. 195; Blois, M.S., Antioxidant determinations by the use of stable free radical (1958) Nature, 26, p. 1199; Kamalakkannan N & Stanley Mainzen Prince P, Effect of Aegle marmelos fruit extract on tissue antioxidants in streptozotocin diabetic rats, Indian J Exp Biol, 41 (2003) 1288; Ray, G., Akhtar Husain, S., Oxidents, antioxidants and carcinogenesis (2002) Indian J Exp Biol, 40, p. 1214; Ialenti, A., Moncada, S., Di Rosa, M., Modulation of adjuvant arthritis by endogenous nitric oxide (1993) Brit J Pharmacol, 110, p. 701; Marcocci, L., Packer, L., Droy-Lefaiz, M.T., Sekaki, A., Gardes- Albert, M., Antioxidant action of Ginko biloba extract Egb 761 (1994) Meth Enzymol, 234, p. 462; Sanchez, M.C., Methods used to evaluate the free radical scavenging activity in foods and biological systems (2002) Food- Sci Tech Int, 8, p. 122",
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Shirwaikar, A, Prabhu, KS & Punitha, ISR 2006, 'In vitro antioxidant studies of Sphaeranthus indicus (Linn)', Indian Journal of Experimental Biology, vol. 44, no. 12, pp. 993-996.

In vitro antioxidant studies of Sphaeranthus indicus (Linn). / Shirwaikar, A.; Prabhu, K.S.; Punitha, I.S.R.

In: Indian Journal of Experimental Biology, Vol. 44, No. 12, 2006, p. 993-996.

Research output: Contribution to journalArticle

TY - JOUR

T1 - In vitro antioxidant studies of Sphaeranthus indicus (Linn)

AU - Shirwaikar, A.

AU - Prabhu, K.S.

AU - Punitha, I.S.R.

N1 - Cited By :62 Export Date: 10 November 2017 CODEN: IJEBA Correspondence Address: Shirwaikar, A.; Department of Pharmacognosy, Manipal College of Pharmaceutical Sciences, Manipal 576 104, India; email: annieshirwaikar@yahoo.com Chemicals/CAS: nitric oxide, 10102-43-9; superoxide dismutase, 37294-21-6, 9016-01-7, 9054-89-1; superoxide, 11062-77-4; Antioxidants; Nitric Oxide, 10102-43-9; Plant Extracts; Superoxide Dismutase, EC 1.15.1.1; Superoxides, 11062-77-4 References: Cross, C.E., Oxygen radicals and human disease (1987) Annal Int Med, 107, p. 526; Marx, J.L., Oxygen free radicals linked to many diseases (1987) Science, 235, p. 529; Ajay Arora, S.R.K., Srivastava, G.C., Oxidative stress and antioxidative system in plants (2002) Curr Sci, 82, p. 1227; Geesin, J.G., Gordon, J.S., Berg, R.A., Retinoids affect collagen synthesis through inhibition of ascorbate- induced lipid peroxidation in cultured human dermal fibroblasts (1990) Arch Biochem Biophys, 278, p. 352; Warrier, P.K., Nambiar, V.P.K., Raman Kutty, C., (2004) Indian Medicinal Plants, 1, p. 180. , Orient Longman, Hyderabad, India; Kokate, C.K., (1986) Practical pharmacognosy, p. 15. , 1st ed, Vallabh Prakashan, New Delhi, India; Yoganarasimhan, S.N., (2000) Medicinal plants of India-Tamil Nadu, 2, p. 512. , Cyber Media, Bangalore, India; Nadkarni, A.K., (2000) Indian materia medica, 1, p. 113. , Popular Prakashan Ltd, Mumbai, India; Shirwaikar Annie, K.R., Dinesh, K., In vitro antioxidant studies of Annona Squamosa (2004) Indian J Exp Biol, 142, p. 803; Sreejayan, N., Rao, M.N.A., Free radical scavenging activity of curcuminoids (1996) Drug Res, 46, p. 169; Sanchez-Moreno, C., Methods used to evaluate the free radical scavenging activity in foods and biological systems (2002) Food Sci Tech Int, 8, p. 122; Henry, L.E.A., Halliwell, B., Hall, D.O., The superoxide dismutase activity of various photosynthetic organisms measured by a new and a rapid assay technique (1976) FEBS Lett, 66, p. 303; Sreejayan, N., Rao, M.N.A., Nitric oxide scavenging by curcuminoids (1997) J Pharm Pharmacol, 49, p. 105; Annie, S., Somashekar, A.P., Antiinflammatory activity and free radical scavenging studies of Aristolochia bracteolata Lam (2003) Indian J Pharm Sci, 65, p. 68; Green, L.C., Wagner, D.A., Glogowski, J., Skipper, P.L., Wishnok, J.S., Tannenbaum, S.R., Analysis of nitrate and 15N in biological fluids (1982) Anal Biochem, 126, p. 131; Marcocci, L., Maguire, J.J., Droy- Lefaix, M.T., Packer, L., The nitric oxide scavenging property of Ginko biloba extract EGB 761 (1994) Biochem Biophys Res Commun, 201, p. 748; Benzie, I.F.F., Strain, J.T., The ferric reducing ability of pLASMA (FRAP) as a measure of "antioxidant power": The FRAP assay (1996) Anal Biochem, 239, p. 70; Benzie, I.F.F., Szeto, Y.T., Total antioxidant capacity of Teas by the Ferric Reducing/ antioxidant power assay (1999) J Agric Food Chem, 47, p. 633; Annie, S., Govindrajan, R., Rastogi, S., Vijaykumar, M., Rawat, A.K.S., ehlotra, S.M., Pushpangadan, P., Studies on the Antioxidant activities of Desmodium gangeticum (2003) Biol Pharm Bull, 26, p. 1424; Youdim, K.A., Joseph, J.A., A possible emerging role of phytochemicals in improving age-related neurological dysfunctions: A multiplicity of effects (2001) Free Rad Biol Med, 30, p. 583; Rice- Evans, C., Miller, N.J., Factors influencing the antioxidant activity determined by the ABTS.+ radical cation assay (1997) Free Rad Res, 26, p. 195; Blois, M.S., Antioxidant determinations by the use of stable free radical (1958) Nature, 26, p. 1199; Kamalakkannan N & Stanley Mainzen Prince P, Effect of Aegle marmelos fruit extract on tissue antioxidants in streptozotocin diabetic rats, Indian J Exp Biol, 41 (2003) 1288; Ray, G., Akhtar Husain, S., Oxidents, antioxidants and carcinogenesis (2002) Indian J Exp Biol, 40, p. 1214; Ialenti, A., Moncada, S., Di Rosa, M., Modulation of adjuvant arthritis by endogenous nitric oxide (1993) Brit J Pharmacol, 110, p. 701; Marcocci, L., Packer, L., Droy-Lefaiz, M.T., Sekaki, A., Gardes- Albert, M., Antioxidant action of Ginko biloba extract Egb 761 (1994) Meth Enzymol, 234, p. 462; Sanchez, M.C., Methods used to evaluate the free radical scavenging activity in foods and biological systems (2002) Food- Sci Tech Int, 8, p. 122

PY - 2006

Y1 - 2006

N2 - The free radical scavenging potential of the plant S.indicus was studied by using different antioxidant models of screening. The ethanolic extract at 1000 μg/ml showed maximum scavenging of the radical cation, 2,2-azinobis-(3- ethylbenzothiazoline-6-sulphonate) (ABTS) observed upto 41.99 % followed by the scavenging of the stable radical 1,1-diphenyl, 2-picryl hydrazyl (DPPH) (33.27 %), superoxide dismutase (25.14 %) and nitric oxide radical (22.36 %) at the same concentration. However, the extract showed only moderate scavenging activity of iron chelation (14.2 %). Total antioxidant capacity of the extract was found to be 160.85 nmol/g ascorbic acid. The results justify the therapeutic applications of the plant in the indigenous system of medicine, augmenting its therapeutic value.

AB - The free radical scavenging potential of the plant S.indicus was studied by using different antioxidant models of screening. The ethanolic extract at 1000 μg/ml showed maximum scavenging of the radical cation, 2,2-azinobis-(3- ethylbenzothiazoline-6-sulphonate) (ABTS) observed upto 41.99 % followed by the scavenging of the stable radical 1,1-diphenyl, 2-picryl hydrazyl (DPPH) (33.27 %), superoxide dismutase (25.14 %) and nitric oxide radical (22.36 %) at the same concentration. However, the extract showed only moderate scavenging activity of iron chelation (14.2 %). Total antioxidant capacity of the extract was found to be 160.85 nmol/g ascorbic acid. The results justify the therapeutic applications of the plant in the indigenous system of medicine, augmenting its therapeutic value.

M3 - Article

VL - 44

SP - 993

EP - 996

JO - Journal of scientific & industrial research. C. Biological sciences

JF - Journal of scientific & industrial research. C. Biological sciences

SN - 0019-5189

IS - 12

ER -