In vitro cytotoxicity of caracemide alone and in combination with hydroxyurea or iron-chelating agents in human chronic myeloid leukemia cells and murine tumors.

K. Satyamoorthy, M. P. Chitnis, S. H. Advani

    Research output: Contribution to journalArticle

    4 Citations (Scopus)

    Abstract

    The cytotoxic effect of caracemide and hydroxyurea was compared in human chronic myeloid leukemia cells. Caracemide was found to be about twelve times more effective than hydroxyurea. The combined effect of caracemide, hydroxyurea and hydrophobic iron-chelating agents at relatively nontoxic concentrations was studied. Hydroxyurea, 2,2'-bipyridine and 1,10-phenanthroline combined with caracemide synergistically inhibited DNA synthesis, while Desferal did not show any such effect. Fe++ partially reversed the cytotoxicity of caracemide in combination with 2,2'-bipyridine, while it had no effect on the cytotoxicity of caracemide alone. Caracemide was found to have a stronger inhibitory effect on DNA synthesis in P388 lymphocytic leukemia and Ehrlich ascites carcinoma cells than hydroxyurea. However, bipyridine, phenanthroline and Desferal in combination with caracemide did not induce any synergistic inhibition. These data indicate the value of human tumor cells to predict drug responsiveness and suggest the further evaluation of caracemide and its combination with hydroxyurea and iron-chelating agents in the treatment of human leukemias.

    Original languageEnglish
    Pages (from-to)27-35
    Number of pages9
    JournalNeoplasma
    Volume35
    Issue number1
    Publication statusPublished - 1988

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    Iron Chelating Agents
    Hydroxyurea
    Myeloid Cells
    Leukemia, Myelogenous, Chronic, BCR-ABL Positive
    Neoplasms
    2,2'-Dipyridyl
    Deferoxamine
    Lymphoid Leukemia
    In Vitro Techniques
    caracemide
    Phenanthrolines
    DNA
    Ascites
    Leukemia
    Carcinoma

    All Science Journal Classification (ASJC) codes

    • Cancer Research

    Cite this

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    abstract = "The cytotoxic effect of caracemide and hydroxyurea was compared in human chronic myeloid leukemia cells. Caracemide was found to be about twelve times more effective than hydroxyurea. The combined effect of caracemide, hydroxyurea and hydrophobic iron-chelating agents at relatively nontoxic concentrations was studied. Hydroxyurea, 2,2'-bipyridine and 1,10-phenanthroline combined with caracemide synergistically inhibited DNA synthesis, while Desferal did not show any such effect. Fe++ partially reversed the cytotoxicity of caracemide in combination with 2,2'-bipyridine, while it had no effect on the cytotoxicity of caracemide alone. Caracemide was found to have a stronger inhibitory effect on DNA synthesis in P388 lymphocytic leukemia and Ehrlich ascites carcinoma cells than hydroxyurea. However, bipyridine, phenanthroline and Desferal in combination with caracemide did not induce any synergistic inhibition. These data indicate the value of human tumor cells to predict drug responsiveness and suggest the further evaluation of caracemide and its combination with hydroxyurea and iron-chelating agents in the treatment of human leukemias.",
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    In vitro cytotoxicity of caracemide alone and in combination with hydroxyurea or iron-chelating agents in human chronic myeloid leukemia cells and murine tumors. / Satyamoorthy, K.; Chitnis, M. P.; Advani, S. H.

    In: Neoplasma, Vol. 35, No. 1, 1988, p. 27-35.

    Research output: Contribution to journalArticle

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