In vitro free radical scavenging potential of methanol extract of entire plant of Phyllanthus reticulatus poir

H.N. Aswatha Ram, C.S. Shreedhara, F.P. Gajera, S.B. Zanwar

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The herb Phyllanthus reticulatus is commonly known as potato bush, which is widely used in the indigenous system of medicine for the treatment of diarrhoea, diabetes, obesity and burns. The methanolic extract of the plant was studied for its in vitro free radical scavenging activity in different methods viz DPPH radical scavenging assay, ABTS radical scavenging assay, Ophenanthroline assay, lipid peroxidation assay, nitric oxide scavenging assay, superoxide scavenging assay, total antioxidant and non-enzymatic haemoglobin glycosylation assay. The results were analyzed statistically by regression method. Its antioxidant activity was estimated by IC 50 value and the values were 14.31 μgm/ml (DPPH radical scavenging), 10.51 μgm/ml (ABTS radical scavenging), 22.91 μgm/ml (O-phenanthroline assay), 32.16 μgm/ml (lipid peroxidation), 90.51 μgm/ml (nitric oxide scavenging) and 4.18 μgm/ml (superoxide scavenging). In total antioxidant capacity assay, 1 mg of extract is equivalent to 41.31 μg of ascorbic acid. It showed 83.69% inhibition of haemoglobin glycosylation.
Original languageEnglish
Pages (from-to)440-451
Number of pages12
JournalPharmacologyonline
Volume2
Publication statusPublished - 2008

Fingerprint

Phyllanthus
Plant Extracts
Free Radicals
Methanol
Antioxidants
Glycosylation
Superoxides
Lipid Peroxidation
Nitric Oxide
Hemoglobins
Phenanthrolines
Traditional Medicine
Solanum tuberosum
Systems Analysis
Burns
Ascorbic Acid
Diarrhea
Obesity
In Vitro Techniques
2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid

Cite this

@article{05d261a11d964978b5716423a93dd655,
title = "In vitro free radical scavenging potential of methanol extract of entire plant of Phyllanthus reticulatus poir",
abstract = "The herb Phyllanthus reticulatus is commonly known as potato bush, which is widely used in the indigenous system of medicine for the treatment of diarrhoea, diabetes, obesity and burns. The methanolic extract of the plant was studied for its in vitro free radical scavenging activity in different methods viz DPPH radical scavenging assay, ABTS radical scavenging assay, Ophenanthroline assay, lipid peroxidation assay, nitric oxide scavenging assay, superoxide scavenging assay, total antioxidant and non-enzymatic haemoglobin glycosylation assay. The results were analyzed statistically by regression method. Its antioxidant activity was estimated by IC 50 value and the values were 14.31 μgm/ml (DPPH radical scavenging), 10.51 μgm/ml (ABTS radical scavenging), 22.91 μgm/ml (O-phenanthroline assay), 32.16 μgm/ml (lipid peroxidation), 90.51 μgm/ml (nitric oxide scavenging) and 4.18 μgm/ml (superoxide scavenging). In total antioxidant capacity assay, 1 mg of extract is equivalent to 41.31 μg of ascorbic acid. It showed 83.69{\%} inhibition of haemoglobin glycosylation.",
author = "{Aswatha Ram}, H.N. and C.S. Shreedhara and F.P. Gajera and S.B. Zanwar",
note = "Cited By :4 Export Date: 10 November 2017 Correspondence Address: Aswatha Ram, H.N.; Department of Pharmacognosy, Manipal College of Pharmaceutical Sciences, Manipal - 576 104 Karnataka, India; email: aswatharam@google.com Chemicals/CAS: 1,1 diphenyl 2 picrylhydrazyl, 1898-66-4; ascorbic acid, 134-03-2, 15421-15-5, 50-81-7; hemoglobin, 9008-02-0; lipid, 66455-18-3; methanol, 67-56-1; nitric oxide, 10102-43-9; phenanthroline, 12678-01-2; superoxide, 11062-77-4; tocopherol, 1406-66-2 References: Mruthunjaja, K., Hukkeri, V.I., Antioxidant and free radical scavenging potential of Justicia gendarussa Burm. Leaves in vitro (2007) Natural Product Sciences, 13 (3), pp. 199-206; (1987) Medicinal Plants of India, 2, p. 407. , ICMR, Indian council of medicinal research, New Delhi; Nadkarni, K.M., Nadkarni, A.K., (1976) Indian Materia Medica, 1, pp. 948-949. , Popular Prakashan Pvt. Ltd, Bombay, India; Longman, O., (2003) Indian medicinal plants, a compendium of 500 species, 4, p. 264; Lam, S.-H., Wang, C.-Y., Chen, C.-K., Lee, S.-S., Phytochemical investigation of Phyllanthus reticulatus by HPLC-SPE-NMR and conventional methods (2007) Phytochemical Analysis, 18, pp. 251-255; Ghani, A., (2003) Medicinal Plants of Bangladesh: Chemical Constituents and Uses, p. 345. , Asiatic Society of Bangladesh, 2nd edition; Kumar, S., Kumar, D., Deshmukh, R.R., Lokhande, P.D., More, S.N., Rangari, V.D., Antidiabetic potential of Phyllanthus reticulatus in alloxan-induced diabetic mice (2008) Fitoterapia, 79, pp. 21-23; Kokate, C.K., Preliminary Phytochemical Screening (1986) Practical Pharmacognosy, pp. 111-115. , Vallabh Prakashan, Delhi, India, 1st Ed; Sreejayan, N., Rao, M.N.A., Free radical scavenging activity of curcuminoids (1996) Drug Res, 46, p. 169; John, A., Steven, D.A., Microsomal lipid peroxidation (1984) Methods in Enzymology, 30, pp. 302-308; Benzie IFF, Strain JT. The ferric reducing ability of plasma (FRAP) as a measure of antioxidant power, the FRAP assay. Anal. Biochem 1996; 239: 70; Sudheerkumar, M., Jagadish, P.C., Sridhar, R.B., Kiran, B.S., Unnikrishnan, M.K., In vitro evaluation of antioxidant properties of Cocus nucifera Linn (2003) Nahrung/Food, 47, pp. 126-131; Govindarajan, R., Vijaya Kumar, M., Rawat, A.K.S., Mehrotra, S., Free radical scavenging potential of Picrrorhiza kurroa Royle ex Benth (2003) Indian J. Exptl. Biol, 41, p. 875; Annie, S., Govindrajan, R., Rastogi, S., Vijaykumar, M., Rawat, A.K.S., Mehlotra, S., Pushpangadan, P., Studies on the Antioxidant activities of Desmodium gangeticum (2003) Biol. Pharm. Bull, 26, p. 1424; Pal, D.K., Dutta, S., Evaluation of the Antioxidant activity of the roots and Rhizomes of Cyperus rotundus L (2006) Indian Journal of Pharmaceutical Sciences, 68, pp. 256-258; Aswatha Ram, H.N., Shreedhara, C.S., Falguni Gajera, P., Sachin Zanwar, B., Antioxidant studies of aqueous extract of Phyllanthus reticulatus Poir (2008) Pharmacologyonline, 1, pp. 351-364; Cross, C.E., (1987) Oxygen radicals and human disease, Annals of Inernal Med, 107, pp. 526-529; Marx, J.L., Oxygen free radicals linked to many diseases (1987) Science, 235, pp. 512-529; Arora, A., Sairam, R.K., Srivastava, G.C., Oxidative stress and antioxidative system in plants (2002) Cur. Sci, 82, pp. 1227-1229; Shirwaikar, A., Shirwaikar, A., Punitha, I.S.R., Antioxidant studies on the methanol stem extract of Coscinium fenestratum (2007) Natural Product Sciences, 13 (1), pp. 40-45; Ganapaty, S., Chandrashekhar, V.M., Chitme, H.R., Lakshmi Narsu, M., Free radical scavenging activity of gossypin and nevadensin : An in vitro relation (2007) Indian journal of Pharmacology, 39, pp. 281-283; Rice-Evans, C., Miller, N.J., Factors affecting the antioxidant activity determined by the ABTS radical cation assay (1997) Free Radic. Res, 195, pp. 26-27; Mahakunakorn, P., Tohda, M., Murakami, Y., Matsumoto, K., Watanabe, H., Antioxidant and free radical scavenging activity of Choto-san and its related constituents (2004) Biol. Pharm. Bull, 27, pp. 38-46; Valentao, P., Fernandes, E., Carvalho, F., Andrade, P.B., Seabra, R.M., Bastos, M.L., Studies on the antioxidant activity of Lippia citriodoru Infusion: Scavenging effect on superoxide radical, hydroxyl radical and hypochlorous acid (2002) Biol. Pharm. Bull, 25, pp. 1324-1327; Hagerman, A.E., Riedl, K.M., Jones, G.A., Sovik, K.N., Ritchard, N.T., Hartzfeld, P.W., High molecular weight plant polyphenolics (tannins) as biological antioxidants (1998) J. Agric. And Food Chem, 46, pp. 1887-1892; Marcocci, L., Packer, L., Droy-Lefaiz, M.T., Sekaki, A., Gardes-Albert, M., Antioxidant actions of Ginko biloba extract Egb 761 (1958) Method in Enzymol, 26, pp. 1199-1202",
year = "2008",
language = "English",
volume = "2",
pages = "440--451",
journal = "Pharmacologyonline",
issn = "1827-8620",
publisher = "SILAE (Italo-Latin American Society of Ethnomedicine)",

}

In vitro free radical scavenging potential of methanol extract of entire plant of Phyllanthus reticulatus poir. / Aswatha Ram, H.N.; Shreedhara, C.S.; Gajera, F.P.; Zanwar, S.B.

In: Pharmacologyonline, Vol. 2, 2008, p. 440-451.

Research output: Contribution to journalArticle

TY - JOUR

T1 - In vitro free radical scavenging potential of methanol extract of entire plant of Phyllanthus reticulatus poir

AU - Aswatha Ram, H.N.

AU - Shreedhara, C.S.

AU - Gajera, F.P.

AU - Zanwar, S.B.

N1 - Cited By :4 Export Date: 10 November 2017 Correspondence Address: Aswatha Ram, H.N.; Department of Pharmacognosy, Manipal College of Pharmaceutical Sciences, Manipal - 576 104 Karnataka, India; email: aswatharam@google.com Chemicals/CAS: 1,1 diphenyl 2 picrylhydrazyl, 1898-66-4; ascorbic acid, 134-03-2, 15421-15-5, 50-81-7; hemoglobin, 9008-02-0; lipid, 66455-18-3; methanol, 67-56-1; nitric oxide, 10102-43-9; phenanthroline, 12678-01-2; superoxide, 11062-77-4; tocopherol, 1406-66-2 References: Mruthunjaja, K., Hukkeri, V.I., Antioxidant and free radical scavenging potential of Justicia gendarussa Burm. Leaves in vitro (2007) Natural Product Sciences, 13 (3), pp. 199-206; (1987) Medicinal Plants of India, 2, p. 407. , ICMR, Indian council of medicinal research, New Delhi; Nadkarni, K.M., Nadkarni, A.K., (1976) Indian Materia Medica, 1, pp. 948-949. , Popular Prakashan Pvt. Ltd, Bombay, India; Longman, O., (2003) Indian medicinal plants, a compendium of 500 species, 4, p. 264; Lam, S.-H., Wang, C.-Y., Chen, C.-K., Lee, S.-S., Phytochemical investigation of Phyllanthus reticulatus by HPLC-SPE-NMR and conventional methods (2007) Phytochemical Analysis, 18, pp. 251-255; Ghani, A., (2003) Medicinal Plants of Bangladesh: Chemical Constituents and Uses, p. 345. , Asiatic Society of Bangladesh, 2nd edition; Kumar, S., Kumar, D., Deshmukh, R.R., Lokhande, P.D., More, S.N., Rangari, V.D., Antidiabetic potential of Phyllanthus reticulatus in alloxan-induced diabetic mice (2008) Fitoterapia, 79, pp. 21-23; Kokate, C.K., Preliminary Phytochemical Screening (1986) Practical Pharmacognosy, pp. 111-115. , Vallabh Prakashan, Delhi, India, 1st Ed; Sreejayan, N., Rao, M.N.A., Free radical scavenging activity of curcuminoids (1996) Drug Res, 46, p. 169; John, A., Steven, D.A., Microsomal lipid peroxidation (1984) Methods in Enzymology, 30, pp. 302-308; Benzie IFF, Strain JT. The ferric reducing ability of plasma (FRAP) as a measure of antioxidant power, the FRAP assay. Anal. Biochem 1996; 239: 70; Sudheerkumar, M., Jagadish, P.C., Sridhar, R.B., Kiran, B.S., Unnikrishnan, M.K., In vitro evaluation of antioxidant properties of Cocus nucifera Linn (2003) Nahrung/Food, 47, pp. 126-131; Govindarajan, R., Vijaya Kumar, M., Rawat, A.K.S., Mehrotra, S., Free radical scavenging potential of Picrrorhiza kurroa Royle ex Benth (2003) Indian J. Exptl. Biol, 41, p. 875; Annie, S., Govindrajan, R., Rastogi, S., Vijaykumar, M., Rawat, A.K.S., Mehlotra, S., Pushpangadan, P., Studies on the Antioxidant activities of Desmodium gangeticum (2003) Biol. Pharm. Bull, 26, p. 1424; Pal, D.K., Dutta, S., Evaluation of the Antioxidant activity of the roots and Rhizomes of Cyperus rotundus L (2006) Indian Journal of Pharmaceutical Sciences, 68, pp. 256-258; Aswatha Ram, H.N., Shreedhara, C.S., Falguni Gajera, P., Sachin Zanwar, B., Antioxidant studies of aqueous extract of Phyllanthus reticulatus Poir (2008) Pharmacologyonline, 1, pp. 351-364; Cross, C.E., (1987) Oxygen radicals and human disease, Annals of Inernal Med, 107, pp. 526-529; Marx, J.L., Oxygen free radicals linked to many diseases (1987) Science, 235, pp. 512-529; Arora, A., Sairam, R.K., Srivastava, G.C., Oxidative stress and antioxidative system in plants (2002) Cur. Sci, 82, pp. 1227-1229; Shirwaikar, A., Shirwaikar, A., Punitha, I.S.R., Antioxidant studies on the methanol stem extract of Coscinium fenestratum (2007) Natural Product Sciences, 13 (1), pp. 40-45; Ganapaty, S., Chandrashekhar, V.M., Chitme, H.R., Lakshmi Narsu, M., Free radical scavenging activity of gossypin and nevadensin : An in vitro relation (2007) Indian journal of Pharmacology, 39, pp. 281-283; Rice-Evans, C., Miller, N.J., Factors affecting the antioxidant activity determined by the ABTS radical cation assay (1997) Free Radic. Res, 195, pp. 26-27; Mahakunakorn, P., Tohda, M., Murakami, Y., Matsumoto, K., Watanabe, H., Antioxidant and free radical scavenging activity of Choto-san and its related constituents (2004) Biol. Pharm. Bull, 27, pp. 38-46; Valentao, P., Fernandes, E., Carvalho, F., Andrade, P.B., Seabra, R.M., Bastos, M.L., Studies on the antioxidant activity of Lippia citriodoru Infusion: Scavenging effect on superoxide radical, hydroxyl radical and hypochlorous acid (2002) Biol. Pharm. Bull, 25, pp. 1324-1327; Hagerman, A.E., Riedl, K.M., Jones, G.A., Sovik, K.N., Ritchard, N.T., Hartzfeld, P.W., High molecular weight plant polyphenolics (tannins) as biological antioxidants (1998) J. Agric. And Food Chem, 46, pp. 1887-1892; Marcocci, L., Packer, L., Droy-Lefaiz, M.T., Sekaki, A., Gardes-Albert, M., Antioxidant actions of Ginko biloba extract Egb 761 (1958) Method in Enzymol, 26, pp. 1199-1202

PY - 2008

Y1 - 2008

N2 - The herb Phyllanthus reticulatus is commonly known as potato bush, which is widely used in the indigenous system of medicine for the treatment of diarrhoea, diabetes, obesity and burns. The methanolic extract of the plant was studied for its in vitro free radical scavenging activity in different methods viz DPPH radical scavenging assay, ABTS radical scavenging assay, Ophenanthroline assay, lipid peroxidation assay, nitric oxide scavenging assay, superoxide scavenging assay, total antioxidant and non-enzymatic haemoglobin glycosylation assay. The results were analyzed statistically by regression method. Its antioxidant activity was estimated by IC 50 value and the values were 14.31 μgm/ml (DPPH radical scavenging), 10.51 μgm/ml (ABTS radical scavenging), 22.91 μgm/ml (O-phenanthroline assay), 32.16 μgm/ml (lipid peroxidation), 90.51 μgm/ml (nitric oxide scavenging) and 4.18 μgm/ml (superoxide scavenging). In total antioxidant capacity assay, 1 mg of extract is equivalent to 41.31 μg of ascorbic acid. It showed 83.69% inhibition of haemoglobin glycosylation.

AB - The herb Phyllanthus reticulatus is commonly known as potato bush, which is widely used in the indigenous system of medicine for the treatment of diarrhoea, diabetes, obesity and burns. The methanolic extract of the plant was studied for its in vitro free radical scavenging activity in different methods viz DPPH radical scavenging assay, ABTS radical scavenging assay, Ophenanthroline assay, lipid peroxidation assay, nitric oxide scavenging assay, superoxide scavenging assay, total antioxidant and non-enzymatic haemoglobin glycosylation assay. The results were analyzed statistically by regression method. Its antioxidant activity was estimated by IC 50 value and the values were 14.31 μgm/ml (DPPH radical scavenging), 10.51 μgm/ml (ABTS radical scavenging), 22.91 μgm/ml (O-phenanthroline assay), 32.16 μgm/ml (lipid peroxidation), 90.51 μgm/ml (nitric oxide scavenging) and 4.18 μgm/ml (superoxide scavenging). In total antioxidant capacity assay, 1 mg of extract is equivalent to 41.31 μg of ascorbic acid. It showed 83.69% inhibition of haemoglobin glycosylation.

M3 - Article

VL - 2

SP - 440

EP - 451

JO - Pharmacologyonline

JF - Pharmacologyonline

SN - 1827-8620

ER -