In vitro matured oocytes are more susceptible than in vivo matured oocytes to mock ICSI induced functional and genetic changes

Shubhashree Uppangala, Shilly Dhiman, Sujit Raj Salian, Vikram Jeet Singh, Guruprasad Kalthur, Satish Kumar Adiga

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Background: Concerns regarding the safety of ICSI have been intensified recently due to increased risk of birth defects in ICSI born children. Although fertilization rate is significantly higher in ICSI cycles, studies have failed to demonstrate the benefits of ICSI in improving the pregnancy rate. Poor technical skill, and suboptimal in vitro conditions may account for the ICSI results however, there is no report on the effects of oocyte manipulations on the ICSI outcome. Objective: The present study elucidates the influence of mock ICSI on the functional and genetic integrity of the mouse oocytes. Methods: Reactive Oxygen Species (ROS) level, mitochondrial status, and phosphorylation of H2AX were assessed in the in vivo matured and IVM oocytes subjected to mock ICSI. Results: A significant increase in ROS level was observed in both in vivo matured and IVM oocytes subjected to mock ICSI (P<0.05-0.001) whereas unique mitochondrial distribution pattern was found only in IVM oocytes (P<0.01-0.001). Importantly, differential H2AX phosphorylation was observed in both in vivo matured and IVM oocytes subjected to mock ICSI (P <0.001). Conclusion: The data from this study suggests that mock ICSI can alter genetic and functional integrity in oocytes and IVM oocytes are more vulnerable to mock ICSI induced changes.

Original languageEnglish
Article numbere0119735
JournalPLoS One
Volume10
Issue number3
DOIs
Publication statusPublished - 18-03-2015

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Phosphorylation
Intracytoplasmic Sperm Injections
Oocytes
Reactive Oxygen Species
oocytes
Defects
reactive oxygen species
phosphorylation
In Vitro Techniques
fertilization (reproduction)
pregnancy rate
Pregnancy Rate
Fertilization
mice

All Science Journal Classification (ASJC) codes

  • Medicine(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

@article{4a253f8492684392aeedd580a6443785,
title = "In vitro matured oocytes are more susceptible than in vivo matured oocytes to mock ICSI induced functional and genetic changes",
abstract = "Background: Concerns regarding the safety of ICSI have been intensified recently due to increased risk of birth defects in ICSI born children. Although fertilization rate is significantly higher in ICSI cycles, studies have failed to demonstrate the benefits of ICSI in improving the pregnancy rate. Poor technical skill, and suboptimal in vitro conditions may account for the ICSI results however, there is no report on the effects of oocyte manipulations on the ICSI outcome. Objective: The present study elucidates the influence of mock ICSI on the functional and genetic integrity of the mouse oocytes. Methods: Reactive Oxygen Species (ROS) level, mitochondrial status, and phosphorylation of H2AX were assessed in the in vivo matured and IVM oocytes subjected to mock ICSI. Results: A significant increase in ROS level was observed in both in vivo matured and IVM oocytes subjected to mock ICSI (P<0.05-0.001) whereas unique mitochondrial distribution pattern was found only in IVM oocytes (P<0.01-0.001). Importantly, differential H2AX phosphorylation was observed in both in vivo matured and IVM oocytes subjected to mock ICSI (P <0.001). Conclusion: The data from this study suggests that mock ICSI can alter genetic and functional integrity in oocytes and IVM oocytes are more vulnerable to mock ICSI induced changes.",
author = "Shubhashree Uppangala and Shilly Dhiman and Salian, {Sujit Raj} and Singh, {Vikram Jeet} and Guruprasad Kalthur and Adiga, {Satish Kumar}",
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In vitro matured oocytes are more susceptible than in vivo matured oocytes to mock ICSI induced functional and genetic changes. / Uppangala, Shubhashree; Dhiman, Shilly; Salian, Sujit Raj; Singh, Vikram Jeet; Kalthur, Guruprasad; Adiga, Satish Kumar.

In: PLoS One, Vol. 10, No. 3, e0119735, 18.03.2015.

Research output: Contribution to journalArticle

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T1 - In vitro matured oocytes are more susceptible than in vivo matured oocytes to mock ICSI induced functional and genetic changes

AU - Uppangala, Shubhashree

AU - Dhiman, Shilly

AU - Salian, Sujit Raj

AU - Singh, Vikram Jeet

AU - Kalthur, Guruprasad

AU - Adiga, Satish Kumar

PY - 2015/3/18

Y1 - 2015/3/18

N2 - Background: Concerns regarding the safety of ICSI have been intensified recently due to increased risk of birth defects in ICSI born children. Although fertilization rate is significantly higher in ICSI cycles, studies have failed to demonstrate the benefits of ICSI in improving the pregnancy rate. Poor technical skill, and suboptimal in vitro conditions may account for the ICSI results however, there is no report on the effects of oocyte manipulations on the ICSI outcome. Objective: The present study elucidates the influence of mock ICSI on the functional and genetic integrity of the mouse oocytes. Methods: Reactive Oxygen Species (ROS) level, mitochondrial status, and phosphorylation of H2AX were assessed in the in vivo matured and IVM oocytes subjected to mock ICSI. Results: A significant increase in ROS level was observed in both in vivo matured and IVM oocytes subjected to mock ICSI (P<0.05-0.001) whereas unique mitochondrial distribution pattern was found only in IVM oocytes (P<0.01-0.001). Importantly, differential H2AX phosphorylation was observed in both in vivo matured and IVM oocytes subjected to mock ICSI (P <0.001). Conclusion: The data from this study suggests that mock ICSI can alter genetic and functional integrity in oocytes and IVM oocytes are more vulnerable to mock ICSI induced changes.

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