In vitro shoot regeneration of gladiolus in semi-solid agar versus liquid cultures with support systems

V. S.S. Prasad, S. Dutta Gupta

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Multiple shoot clusters of gladiolus were established in semi-solid agar (AS) and liquid media appended with membrane raft (MR) and Duroplast foam (DF) support matrix. A difference in optimum combination of α-napthaleneacetic acid (NAA)/6-benzylaminopurine (BAP) for shoot multiplication was noted with culture systems. Maximum regeneration of 33.15 shoots/cluster was obtained with 1 mg l-1 NAA and 2 mg l-1 BAP in liquid medium with membrane support. There were 33.46 and 25.37% increase in shoot regeneration in MR system than AS and DF systems, respectively. Shoot multiplication was rapid in AS and MR systems (relative differentiation rate of 5.7% per 7 days of incubation) when compared to DF system (relative differentiation rate of 4.6% per 7 days of incubation). Shoot multiplication followed a linear trend with incubation time, whereas shoot elongation fitted well with quadratic functions. Such analysis suggests that shoot number increases steadily with time, whereas the rate of shoot elongation was different at different phases of incubation in different culture systems. Shoots in DF system exhibited faster elongation than those in AS and MR systems.

Original languageEnglish
Pages (from-to)263-271
Number of pages9
JournalPlant Cell, Tissue and Organ Culture
Volume87
Issue number3
DOIs
Publication statusPublished - 01-12-2006
Externally publishedYes

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Gladiolus
support systems
agar
shoots
liquids
foams
acids
benzyladenine

All Science Journal Classification (ASJC) codes

  • Horticulture

Cite this

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abstract = "Multiple shoot clusters of gladiolus were established in semi-solid agar (AS) and liquid media appended with membrane raft (MR) and Duroplast foam (DF) support matrix. A difference in optimum combination of α-napthaleneacetic acid (NAA)/6-benzylaminopurine (BAP) for shoot multiplication was noted with culture systems. Maximum regeneration of 33.15 shoots/cluster was obtained with 1 mg l-1 NAA and 2 mg l-1 BAP in liquid medium with membrane support. There were 33.46 and 25.37{\%} increase in shoot regeneration in MR system than AS and DF systems, respectively. Shoot multiplication was rapid in AS and MR systems (relative differentiation rate of 5.7{\%} per 7 days of incubation) when compared to DF system (relative differentiation rate of 4.6{\%} per 7 days of incubation). Shoot multiplication followed a linear trend with incubation time, whereas shoot elongation fitted well with quadratic functions. Such analysis suggests that shoot number increases steadily with time, whereas the rate of shoot elongation was different at different phases of incubation in different culture systems. Shoots in DF system exhibited faster elongation than those in AS and MR systems.",
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In vitro shoot regeneration of gladiolus in semi-solid agar versus liquid cultures with support systems. / Prasad, V. S.S.; Gupta, S. Dutta.

In: Plant Cell, Tissue and Organ Culture, Vol. 87, No. 3, 01.12.2006, p. 263-271.

Research output: Contribution to journalArticle

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