In-vitro susceptibility of dermatophytes to ketoconazole, fluconazole and tolnaftate

Sumit Kumar, Pallavi Kumari, P. Mallya Shrikara, Shenoy Shalini

Research output: Contribution to journalArticle

Abstract

The present study was undertaken with following aim and objectives. Isolation and identification of different aetiological agents causing dermatophytosis and to study antifungal susceptibility testing of isolated fungi. Isolation and identification was done by macroscopic, microscopic and biochemical tests.In-vitro drug susceptibility testing was done by broth macro dilution method The present study for isolation, identification and in-vitro drug susceptibility testing was done on 250 clinically diagnosed cases of dermatophytosis. Out of 250 cases of dermatophytosis, 138 cases (55.2%) were positive in direct microscopic examination (KOH) and total of 106 cases (42.4%) were positive in culture. 102cases (40.80%) were positive in direct examination (KOH).MIC range of ketoconazole for all the isolates was 0.25-8μg/ml.MIC range for all the isolates against fluconazole was 0.25-16μg/ml.MIC range for all the isolates against tolnaftate was 0.5-> 32μg/ml. This study highlighted that,in vitro susceptibility pattern indicates that dermatophytes are more susceptible to ketoconozole and fluconazole than tolnaftate.

Original languageEnglish
Pages (from-to)1345-1350
Number of pages6
JournalJournal of Pure and Applied Microbiology
Volume6
Issue number3
Publication statusPublished - 01-09-2012
Externally publishedYes

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All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Microbiology
  • Applied Microbiology and Biotechnology

Cite this

Kumar, S., Kumari, P., Shrikara, P. M., & Shalini, S. (2012). In-vitro susceptibility of dermatophytes to ketoconazole, fluconazole and tolnaftate. Journal of Pure and Applied Microbiology, 6(3), 1345-1350.