In vivo chromosome damaging effects of an inosine monophosphate dehydrogenase inhibitor

Ribavirin in mice

K. P. Seetharama Rao, K. Narayana

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Objective: To investigate the in vivo mutagenic effects of ribavirin in mice. Methods: Mice were injected (i.p.) 20, 100, or 200 mg/kg ribavirin (single exposure) for bone marrow micronucleus, peripheral blood micronucleus and bone marrow chromosome aberration tests. Five treatments of 200 mg/kg ribavirin was given (i.p.) for sperm morphology test. The tests were performed as per the standard procedures. Results: Ribavirin induced significant number of micronucleated polychromatic erythrocytes (MNPCEs) at 24, 48 and 72 h following the exposure with more effects at 24 h (p<0.05-0.001). Micronucleated PCEs were more at 48 h in lower dose-levels and at 72 h in highest dose-level in the peripheral blood (p<0.05-0.001). Ribavirin induced structural chromosomal damage hence producing the fragments for the micronucleus formation. Ribavirin decreased the PCE%, P/N ratio and the mitotic index indicating that it prevents cell division in mouse bone marrow. Ribavirin also decreased the testis weight and induced the formation of abnormal sperms. Conclusion: Ribavirin is a potent mutagen and cytotoxic agent in mice in vivo. Further, it also induces point mutations in germ cells yielding abnormal sperms. The genotoxic effects of ribavirin are not exerted in a dose-dependent pattern in mouse.

Original languageEnglish
Pages (from-to)90-95
Number of pages6
JournalIndian Journal of Pharmacology
Volume37
Issue number2
Publication statusPublished - 01-03-2005
Externally publishedYes

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Inosine Monophosphate
Ribavirin
Oxidoreductases
Chromosomes
Spermatozoa
Bone Marrow
Mitotic Index
Cytotoxins
Mutagens
Point Mutation
Germ Cells
Chromosome Aberrations
Cell Division
Testis
Erythrocytes
Weights and Measures

All Science Journal Classification (ASJC) codes

  • Pharmacology
  • Pharmacology (medical)

Cite this

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abstract = "Objective: To investigate the in vivo mutagenic effects of ribavirin in mice. Methods: Mice were injected (i.p.) 20, 100, or 200 mg/kg ribavirin (single exposure) for bone marrow micronucleus, peripheral blood micronucleus and bone marrow chromosome aberration tests. Five treatments of 200 mg/kg ribavirin was given (i.p.) for sperm morphology test. The tests were performed as per the standard procedures. Results: Ribavirin induced significant number of micronucleated polychromatic erythrocytes (MNPCEs) at 24, 48 and 72 h following the exposure with more effects at 24 h (p<0.05-0.001). Micronucleated PCEs were more at 48 h in lower dose-levels and at 72 h in highest dose-level in the peripheral blood (p<0.05-0.001). Ribavirin induced structural chromosomal damage hence producing the fragments for the micronucleus formation. Ribavirin decreased the PCE{\%}, P/N ratio and the mitotic index indicating that it prevents cell division in mouse bone marrow. Ribavirin also decreased the testis weight and induced the formation of abnormal sperms. Conclusion: Ribavirin is a potent mutagen and cytotoxic agent in mice in vivo. Further, it also induces point mutations in germ cells yielding abnormal sperms. The genotoxic effects of ribavirin are not exerted in a dose-dependent pattern in mouse.",
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In vivo chromosome damaging effects of an inosine monophosphate dehydrogenase inhibitor : Ribavirin in mice. / Seetharama Rao, K. P.; Narayana, K.

In: Indian Journal of Pharmacology, Vol. 37, No. 2, 01.03.2005, p. 90-95.

Research output: Contribution to journalArticle

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AB - Objective: To investigate the in vivo mutagenic effects of ribavirin in mice. Methods: Mice were injected (i.p.) 20, 100, or 200 mg/kg ribavirin (single exposure) for bone marrow micronucleus, peripheral blood micronucleus and bone marrow chromosome aberration tests. Five treatments of 200 mg/kg ribavirin was given (i.p.) for sperm morphology test. The tests were performed as per the standard procedures. Results: Ribavirin induced significant number of micronucleated polychromatic erythrocytes (MNPCEs) at 24, 48 and 72 h following the exposure with more effects at 24 h (p<0.05-0.001). Micronucleated PCEs were more at 48 h in lower dose-levels and at 72 h in highest dose-level in the peripheral blood (p<0.05-0.001). Ribavirin induced structural chromosomal damage hence producing the fragments for the micronucleus formation. Ribavirin decreased the PCE%, P/N ratio and the mitotic index indicating that it prevents cell division in mouse bone marrow. Ribavirin also decreased the testis weight and induced the formation of abnormal sperms. Conclusion: Ribavirin is a potent mutagen and cytotoxic agent in mice in vivo. Further, it also induces point mutations in germ cells yielding abnormal sperms. The genotoxic effects of ribavirin are not exerted in a dose-dependent pattern in mouse.

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