In vivo intra- and inter-individual variability study of human stratum corneum by confocal Raman spectroscopy

Laurita dos Santos, Joao Lucas Rangel, Vamshi Krishna Tippavajhala, Michely Glenda Pereira da Silva, Borys Mogilevych, Airton Abrahão Martin

Research output: Contribution to journalArticle

Abstract

The present research work deals with the in vivo determination of intra- and inter-individual human stratum corneum biochemical variability using confocal Raman spectroscopy. This study was carried out at three different spots on the volar forearm region of healthy human participants in the extended fingerprint and high wavenumber spectral regions. All the preprocessed spectra were compared by correlation matrices, which were calculated using Pearson's correlation coefficient (r). The results indicate that the participated volunteers in this study are homogenous with respect to the stratum corneum in the forearm region in both extended fingerprint and high wavenumber regions as there is high correlation between their Raman spectra at both intra- and inter-individual levels. This study confirms the application of confocal Raman spectroscopy as an accurate and precise technique for in vivo studies involving human skin.

Original languageEnglish
Pages (from-to)199-206
Number of pages8
JournalVibrational Spectroscopy
Volume87
DOIs
Publication statusPublished - 01-11-2016

Fingerprint

Raman spectroscopy
Raman scattering
Skin

All Science Journal Classification (ASJC) codes

  • Spectroscopy

Cite this

dos Santos, Laurita ; Rangel, Joao Lucas ; Tippavajhala, Vamshi Krishna ; da Silva, Michely Glenda Pereira ; Mogilevych, Borys ; Martin, Airton Abrahão. / In vivo intra- and inter-individual variability study of human stratum corneum by confocal Raman spectroscopy. In: Vibrational Spectroscopy. 2016 ; Vol. 87. pp. 199-206.
@article{2c601dbe2e35442a91ab0808c0f24d76,
title = "In vivo intra- and inter-individual variability study of human stratum corneum by confocal Raman spectroscopy",
abstract = "The present research work deals with the in vivo determination of intra- and inter-individual human stratum corneum biochemical variability using confocal Raman spectroscopy. This study was carried out at three different spots on the volar forearm region of healthy human participants in the extended fingerprint and high wavenumber spectral regions. All the preprocessed spectra were compared by correlation matrices, which were calculated using Pearson's correlation coefficient (r). The results indicate that the participated volunteers in this study are homogenous with respect to the stratum corneum in the forearm region in both extended fingerprint and high wavenumber regions as there is high correlation between their Raman spectra at both intra- and inter-individual levels. This study confirms the application of confocal Raman spectroscopy as an accurate and precise technique for in vivo studies involving human skin.",
author = "{dos Santos}, Laurita and Rangel, {Joao Lucas} and Tippavajhala, {Vamshi Krishna} and {da Silva}, {Michely Glenda Pereira} and Borys Mogilevych and Martin, {Airton Abrah{\~a}o}",
year = "2016",
month = "11",
day = "1",
doi = "10.1016/j.vibspec.2016.10.007",
language = "English",
volume = "87",
pages = "199--206",
journal = "Vibrational Spectroscopy",
issn = "0924-2031",
publisher = "Elsevier",

}

In vivo intra- and inter-individual variability study of human stratum corneum by confocal Raman spectroscopy. / dos Santos, Laurita; Rangel, Joao Lucas; Tippavajhala, Vamshi Krishna; da Silva, Michely Glenda Pereira; Mogilevych, Borys; Martin, Airton Abrahão.

In: Vibrational Spectroscopy, Vol. 87, 01.11.2016, p. 199-206.

Research output: Contribution to journalArticle

TY - JOUR

T1 - In vivo intra- and inter-individual variability study of human stratum corneum by confocal Raman spectroscopy

AU - dos Santos, Laurita

AU - Rangel, Joao Lucas

AU - Tippavajhala, Vamshi Krishna

AU - da Silva, Michely Glenda Pereira

AU - Mogilevych, Borys

AU - Martin, Airton Abrahão

PY - 2016/11/1

Y1 - 2016/11/1

N2 - The present research work deals with the in vivo determination of intra- and inter-individual human stratum corneum biochemical variability using confocal Raman spectroscopy. This study was carried out at three different spots on the volar forearm region of healthy human participants in the extended fingerprint and high wavenumber spectral regions. All the preprocessed spectra were compared by correlation matrices, which were calculated using Pearson's correlation coefficient (r). The results indicate that the participated volunteers in this study are homogenous with respect to the stratum corneum in the forearm region in both extended fingerprint and high wavenumber regions as there is high correlation between their Raman spectra at both intra- and inter-individual levels. This study confirms the application of confocal Raman spectroscopy as an accurate and precise technique for in vivo studies involving human skin.

AB - The present research work deals with the in vivo determination of intra- and inter-individual human stratum corneum biochemical variability using confocal Raman spectroscopy. This study was carried out at three different spots on the volar forearm region of healthy human participants in the extended fingerprint and high wavenumber spectral regions. All the preprocessed spectra were compared by correlation matrices, which were calculated using Pearson's correlation coefficient (r). The results indicate that the participated volunteers in this study are homogenous with respect to the stratum corneum in the forearm region in both extended fingerprint and high wavenumber regions as there is high correlation between their Raman spectra at both intra- and inter-individual levels. This study confirms the application of confocal Raman spectroscopy as an accurate and precise technique for in vivo studies involving human skin.

UR - http://www.scopus.com/inward/record.url?scp=84994029423&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84994029423&partnerID=8YFLogxK

U2 - 10.1016/j.vibspec.2016.10.007

DO - 10.1016/j.vibspec.2016.10.007

M3 - Article

VL - 87

SP - 199

EP - 206

JO - Vibrational Spectroscopy

JF - Vibrational Spectroscopy

SN - 0924-2031

ER -