Abstract
This research work mainly deals with studying qualitatively the changes in the dermal collagen of two forms of striae distensae (SD) namely striae rubrae (SR) and striae albae (SA) when compared to normal skin (NS) using confocal Raman spectroscopy. The methodology includes an in vivo human skin study for the comparison of confocal Raman spectra of dermis region of SR, SA, and NS by supervised multivariate analysis using partial least squares discriminant analysis (PLS-DA) to determine qualitatively the changes in dermal collagen. These groups are further analyzed for the extent of hydration of dermal collagen by studying the changes in the water content bound to it. PLS-DA score plot showed good separation of the confocal Raman spectra of dermis region into SR, SA, and NS data groups. Further analysis using loading plot and S-plot indicated the participation of various components of dermal collagen in the separation of these groups. Bound water content analysis showed that the extent of hydration of collagen is more in SD when compared to NS. Based on the results obtained, this study confirms the active involvement of dermal collagen in the formation of SD. It also emphasizes the need to study quantitatively the role of these various biochemical changes in the dermal collagen responsible for the variance between SR, SA, and NS.
| Original language | English |
|---|---|
| Pages (from-to) | 609-617 |
| Number of pages | 9 |
| Journal | Lasers in Medical Science |
| Volume | 33 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - 01-04-2018 |
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All Science Journal Classification (ASJC) codes
- Surgery
- Dermatology
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In vivo study of dermal collagen of striae distensae by confocal Raman spectroscopy. / Lung, Pam Wen; Tippavajhala, Vamshi Krishna; de Oliveira Mendes, Thiago; Téllez-Soto, Claudio A.; Schuck, Desirée Cigaran; Brohem, Carla Abdo; Lorencini, Marcio; Martin, Airton Abrahão.
In: Lasers in Medical Science, Vol. 33, No. 3, 01.04.2018, p. 609-617.Research output: Contribution to journal › Article
TY - JOUR
T1 - In vivo study of dermal collagen of striae distensae by confocal Raman spectroscopy
AU - Lung, Pam Wen
AU - Tippavajhala, Vamshi Krishna
AU - de Oliveira Mendes, Thiago
AU - Téllez-Soto, Claudio A.
AU - Schuck, Desirée Cigaran
AU - Brohem, Carla Abdo
AU - Lorencini, Marcio
AU - Martin, Airton Abrahão
PY - 2018/4/1
Y1 - 2018/4/1
N2 - This research work mainly deals with studying qualitatively the changes in the dermal collagen of two forms of striae distensae (SD) namely striae rubrae (SR) and striae albae (SA) when compared to normal skin (NS) using confocal Raman spectroscopy. The methodology includes an in vivo human skin study for the comparison of confocal Raman spectra of dermis region of SR, SA, and NS by supervised multivariate analysis using partial least squares discriminant analysis (PLS-DA) to determine qualitatively the changes in dermal collagen. These groups are further analyzed for the extent of hydration of dermal collagen by studying the changes in the water content bound to it. PLS-DA score plot showed good separation of the confocal Raman spectra of dermis region into SR, SA, and NS data groups. Further analysis using loading plot and S-plot indicated the participation of various components of dermal collagen in the separation of these groups. Bound water content analysis showed that the extent of hydration of collagen is more in SD when compared to NS. Based on the results obtained, this study confirms the active involvement of dermal collagen in the formation of SD. It also emphasizes the need to study quantitatively the role of these various biochemical changes in the dermal collagen responsible for the variance between SR, SA, and NS.
AB - This research work mainly deals with studying qualitatively the changes in the dermal collagen of two forms of striae distensae (SD) namely striae rubrae (SR) and striae albae (SA) when compared to normal skin (NS) using confocal Raman spectroscopy. The methodology includes an in vivo human skin study for the comparison of confocal Raman spectra of dermis region of SR, SA, and NS by supervised multivariate analysis using partial least squares discriminant analysis (PLS-DA) to determine qualitatively the changes in dermal collagen. These groups are further analyzed for the extent of hydration of dermal collagen by studying the changes in the water content bound to it. PLS-DA score plot showed good separation of the confocal Raman spectra of dermis region into SR, SA, and NS data groups. Further analysis using loading plot and S-plot indicated the participation of various components of dermal collagen in the separation of these groups. Bound water content analysis showed that the extent of hydration of collagen is more in SD when compared to NS. Based on the results obtained, this study confirms the active involvement of dermal collagen in the formation of SD. It also emphasizes the need to study quantitatively the role of these various biochemical changes in the dermal collagen responsible for the variance between SR, SA, and NS.
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UR - http://www.scopus.com/inward/citedby.url?scp=85044305846&partnerID=8YFLogxK
U2 - 10.1007/s10103-017-2431-3
DO - 10.1007/s10103-017-2431-3
M3 - Article
AN - SCOPUS:85044305846
VL - 33
SP - 609
EP - 617
JO - Lasers in Medical Science
JF - Lasers in Medical Science
SN - 0268-8921
IS - 3
ER -