In vivo study of dermal collagen of striae distensae by confocal Raman spectroscopy

Pam Wen Lung, Vamshi Krishna Tippavajhala, Thiago de Oliveira Mendes, Claudio A. Téllez-Soto, Desirée Cigaran Schuck, Carla Abdo Brohem, Marcio Lorencini, Airton Abrahão Martin

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

This research work mainly deals with studying qualitatively the changes in the dermal collagen of two forms of striae distensae (SD) namely striae rubrae (SR) and striae albae (SA) when compared to normal skin (NS) using confocal Raman spectroscopy. The methodology includes an in vivo human skin study for the comparison of confocal Raman spectra of dermis region of SR, SA, and NS by supervised multivariate analysis using partial least squares discriminant analysis (PLS-DA) to determine qualitatively the changes in dermal collagen. These groups are further analyzed for the extent of hydration of dermal collagen by studying the changes in the water content bound to it. PLS-DA score plot showed good separation of the confocal Raman spectra of dermis region into SR, SA, and NS data groups. Further analysis using loading plot and S-plot indicated the participation of various components of dermal collagen in the separation of these groups. Bound water content analysis showed that the extent of hydration of collagen is more in SD when compared to NS. Based on the results obtained, this study confirms the active involvement of dermal collagen in the formation of SD. It also emphasizes the need to study quantitatively the role of these various biochemical changes in the dermal collagen responsible for the variance between SR, SA, and NS.

Original languageEnglish
Pages (from-to)609-617
Number of pages9
JournalLasers in Medical Science
Volume33
Issue number3
DOIs
Publication statusPublished - 01-04-2018

Fingerprint

Striae Distensae
Raman Spectrum Analysis
Collagen
Skin
Discriminant Analysis
Dermis
Least-Squares Analysis
Water

All Science Journal Classification (ASJC) codes

  • Surgery
  • Dermatology

Cite this

Lung, P. W., Tippavajhala, V. K., de Oliveira Mendes, T., Téllez-Soto, C. A., Schuck, D. C., Brohem, C. A., ... Martin, A. A. (2018). In vivo study of dermal collagen of striae distensae by confocal Raman spectroscopy. Lasers in Medical Science, 33(3), 609-617. https://doi.org/10.1007/s10103-017-2431-3
Lung, Pam Wen ; Tippavajhala, Vamshi Krishna ; de Oliveira Mendes, Thiago ; Téllez-Soto, Claudio A. ; Schuck, Desirée Cigaran ; Brohem, Carla Abdo ; Lorencini, Marcio ; Martin, Airton Abrahão. / In vivo study of dermal collagen of striae distensae by confocal Raman spectroscopy. In: Lasers in Medical Science. 2018 ; Vol. 33, No. 3. pp. 609-617.
@article{409f02ad4a1143e79d53d188cf2a8ecf,
title = "In vivo study of dermal collagen of striae distensae by confocal Raman spectroscopy",
abstract = "This research work mainly deals with studying qualitatively the changes in the dermal collagen of two forms of striae distensae (SD) namely striae rubrae (SR) and striae albae (SA) when compared to normal skin (NS) using confocal Raman spectroscopy. The methodology includes an in vivo human skin study for the comparison of confocal Raman spectra of dermis region of SR, SA, and NS by supervised multivariate analysis using partial least squares discriminant analysis (PLS-DA) to determine qualitatively the changes in dermal collagen. These groups are further analyzed for the extent of hydration of dermal collagen by studying the changes in the water content bound to it. PLS-DA score plot showed good separation of the confocal Raman spectra of dermis region into SR, SA, and NS data groups. Further analysis using loading plot and S-plot indicated the participation of various components of dermal collagen in the separation of these groups. Bound water content analysis showed that the extent of hydration of collagen is more in SD when compared to NS. Based on the results obtained, this study confirms the active involvement of dermal collagen in the formation of SD. It also emphasizes the need to study quantitatively the role of these various biochemical changes in the dermal collagen responsible for the variance between SR, SA, and NS.",
author = "Lung, {Pam Wen} and Tippavajhala, {Vamshi Krishna} and {de Oliveira Mendes}, Thiago and T{\'e}llez-Soto, {Claudio A.} and Schuck, {Desir{\'e}e Cigaran} and Brohem, {Carla Abdo} and Marcio Lorencini and Martin, {Airton Abrah{\~a}o}",
year = "2018",
month = "4",
day = "1",
doi = "10.1007/s10103-017-2431-3",
language = "English",
volume = "33",
pages = "609--617",
journal = "Lasers in Medical Science",
issn = "0268-8921",
publisher = "Springer London",
number = "3",

}

Lung, PW, Tippavajhala, VK, de Oliveira Mendes, T, Téllez-Soto, CA, Schuck, DC, Brohem, CA, Lorencini, M & Martin, AA 2018, 'In vivo study of dermal collagen of striae distensae by confocal Raman spectroscopy', Lasers in Medical Science, vol. 33, no. 3, pp. 609-617. https://doi.org/10.1007/s10103-017-2431-3

In vivo study of dermal collagen of striae distensae by confocal Raman spectroscopy. / Lung, Pam Wen; Tippavajhala, Vamshi Krishna; de Oliveira Mendes, Thiago; Téllez-Soto, Claudio A.; Schuck, Desirée Cigaran; Brohem, Carla Abdo; Lorencini, Marcio; Martin, Airton Abrahão.

In: Lasers in Medical Science, Vol. 33, No. 3, 01.04.2018, p. 609-617.

Research output: Contribution to journalArticle

TY - JOUR

T1 - In vivo study of dermal collagen of striae distensae by confocal Raman spectroscopy

AU - Lung, Pam Wen

AU - Tippavajhala, Vamshi Krishna

AU - de Oliveira Mendes, Thiago

AU - Téllez-Soto, Claudio A.

AU - Schuck, Desirée Cigaran

AU - Brohem, Carla Abdo

AU - Lorencini, Marcio

AU - Martin, Airton Abrahão

PY - 2018/4/1

Y1 - 2018/4/1

N2 - This research work mainly deals with studying qualitatively the changes in the dermal collagen of two forms of striae distensae (SD) namely striae rubrae (SR) and striae albae (SA) when compared to normal skin (NS) using confocal Raman spectroscopy. The methodology includes an in vivo human skin study for the comparison of confocal Raman spectra of dermis region of SR, SA, and NS by supervised multivariate analysis using partial least squares discriminant analysis (PLS-DA) to determine qualitatively the changes in dermal collagen. These groups are further analyzed for the extent of hydration of dermal collagen by studying the changes in the water content bound to it. PLS-DA score plot showed good separation of the confocal Raman spectra of dermis region into SR, SA, and NS data groups. Further analysis using loading plot and S-plot indicated the participation of various components of dermal collagen in the separation of these groups. Bound water content analysis showed that the extent of hydration of collagen is more in SD when compared to NS. Based on the results obtained, this study confirms the active involvement of dermal collagen in the formation of SD. It also emphasizes the need to study quantitatively the role of these various biochemical changes in the dermal collagen responsible for the variance between SR, SA, and NS.

AB - This research work mainly deals with studying qualitatively the changes in the dermal collagen of two forms of striae distensae (SD) namely striae rubrae (SR) and striae albae (SA) when compared to normal skin (NS) using confocal Raman spectroscopy. The methodology includes an in vivo human skin study for the comparison of confocal Raman spectra of dermis region of SR, SA, and NS by supervised multivariate analysis using partial least squares discriminant analysis (PLS-DA) to determine qualitatively the changes in dermal collagen. These groups are further analyzed for the extent of hydration of dermal collagen by studying the changes in the water content bound to it. PLS-DA score plot showed good separation of the confocal Raman spectra of dermis region into SR, SA, and NS data groups. Further analysis using loading plot and S-plot indicated the participation of various components of dermal collagen in the separation of these groups. Bound water content analysis showed that the extent of hydration of collagen is more in SD when compared to NS. Based on the results obtained, this study confirms the active involvement of dermal collagen in the formation of SD. It also emphasizes the need to study quantitatively the role of these various biochemical changes in the dermal collagen responsible for the variance between SR, SA, and NS.

UR - http://www.scopus.com/inward/record.url?scp=85044305846&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85044305846&partnerID=8YFLogxK

U2 - 10.1007/s10103-017-2431-3

DO - 10.1007/s10103-017-2431-3

M3 - Article

AN - SCOPUS:85044305846

VL - 33

SP - 609

EP - 617

JO - Lasers in Medical Science

JF - Lasers in Medical Science

SN - 0268-8921

IS - 3

ER -