Inducible protective processes in animal systems. X. Influence of nicotinamide in methyl methanesulfonate-adapted mouse bone marrow cells

K. P. Guruprasad, V. Vasudev, M. N. Anilkumar, S. A. Chethan

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The adaptive response is an error-free DNA repair mechanism induced by low levels of physical or chemical agents. Cells pre-exposed to such agents are resistant to genetic damage induced by subsequent treatment at a high dose. There are many reports on such adaptive responses. Recently we have shown the existence of adaptive responses in vivo in the grasshopper Poecilocerus pictus and the mouse and in vitro in human lymphocytes. Different enzymes are implicated in this DNA repair pathway. In an attempt to understand the molecular mechanism of the methyl methanesulfonate (MMS)-induced adaptive response, the present investigations have been undertaken employing nicotinamide, an inhibitor of the DNA repair enzyme poly(ADP-ribose) polymerase (PARP). Pre-, inter- and post-treatments with nicotinamide of MMS-treated mouse bone marrow cells were carried out. The results revealed that there is a significant reduction in the frequency of chromosomal aberrations compared with combined treatment, suggesting an enhancement of the adaptive response by nicotinamide. Further, the results of NAD+ assay in the inter-treatment experiment showed that there is no depletion of NAD+. Thus, it can be stated that PARP is not involved in the MMS-induced adaptive response in mouse bone marrow cells.

Original languageEnglish
Pages (from-to)1-8
Number of pages8
JournalMutagenesis
Volume17
Issue number1
Publication statusPublished - 2002

Fingerprint

Methyl Methanesulfonate
Niacinamide
Bone Marrow Cells
Bone
Animals
Poly(ADP-ribose) Polymerases
Cells
DNA Repair
NAD
Repair
DNA Repair Enzymes
Grasshoppers
Lymphocytes
DNA
Aberrations
Chromosome Aberrations
Assays
Enzymes
Experiments

All Science Journal Classification (ASJC) codes

  • Toxicology
  • Genetics
  • Genetics(clinical)
  • Health, Toxicology and Mutagenesis

Cite this

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Inducible protective processes in animal systems. X. Influence of nicotinamide in methyl methanesulfonate-adapted mouse bone marrow cells. / Guruprasad, K. P.; Vasudev, V.; Anilkumar, M. N.; Chethan, S. A.

In: Mutagenesis, Vol. 17, No. 1, 2002, p. 1-8.

Research output: Contribution to journalArticle

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