Induction of Developmental Toxicity in Mice Treated with Alstonia scholaris (Sapthaparna) in Utero

Ganesh Chandra Jagetia, Manjeshwar Shrinath Baliga

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

BACKGROUND: The teratogenic effect of hydroalcoholic extract of Alstonia scholaris (ASE) was studied in the pregnant Swiss albino mice administered with 0, 60, 120, 240, 360, and 480 mg/kg ASE on Day 11 of gestation. METHODS: Females were allowed to complete the term and parturiate. The litters were monitored regularly for mortality, growth retardation, congenital malformations, and appearance of physiological markers up to 7 weeks post-parturition (p.p.). RESULTS: The administration of 60, 120, 180, and 240 mg/kg ASE to the pregnant mice on Day 11 did not induce mortality, congenital malformations, or alter the normal growth patterns. A further increase in the herbal extract dose up to 360 or 480 mg/kg resulted in a dose dependent increase in the mortality, growth retardation, and congenital malformations, characterized mainly by bent tails and syndactyly. The administration of higher doses (360 or 480 mg) of ASE also caused a significant delay in the morphological parameters such as fur development, eye opening, pinna detachment, and vaginal opening. The incisor eruption and testes decent were found to be delayed in litters born to the mothers treated with 240-480 mg/kg ASE. CONCLUSIONS: Our study indicates clearly that ASE treatment caused teratogenic effect only at doses above 240 mg/kg (> 20% of LD50). Lower doses had no developmental toxicity.

Original languageEnglish
Pages (from-to)472-478
Number of pages7
JournalBirth Defects Research Part B - Developmental and Reproductive Toxicology
Volume68
Issue number6
DOIs
Publication statusPublished - 01-12-2003
Externally publishedYes

Fingerprint

Alstonia
Toxicity
Mortality
Growth
Syndactyly
Lethal Dose 50
Incisor
Tail
Testis
Parturition
Pregnancy

All Science Journal Classification (ASJC) codes

  • Genetics
  • Toxicology
  • Cancer Research

Cite this

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title = "Induction of Developmental Toxicity in Mice Treated with Alstonia scholaris (Sapthaparna) in Utero",
abstract = "BACKGROUND: The teratogenic effect of hydroalcoholic extract of Alstonia scholaris (ASE) was studied in the pregnant Swiss albino mice administered with 0, 60, 120, 240, 360, and 480 mg/kg ASE on Day 11 of gestation. METHODS: Females were allowed to complete the term and parturiate. The litters were monitored regularly for mortality, growth retardation, congenital malformations, and appearance of physiological markers up to 7 weeks post-parturition (p.p.). RESULTS: The administration of 60, 120, 180, and 240 mg/kg ASE to the pregnant mice on Day 11 did not induce mortality, congenital malformations, or alter the normal growth patterns. A further increase in the herbal extract dose up to 360 or 480 mg/kg resulted in a dose dependent increase in the mortality, growth retardation, and congenital malformations, characterized mainly by bent tails and syndactyly. The administration of higher doses (360 or 480 mg) of ASE also caused a significant delay in the morphological parameters such as fur development, eye opening, pinna detachment, and vaginal opening. The incisor eruption and testes decent were found to be delayed in litters born to the mothers treated with 240-480 mg/kg ASE. CONCLUSIONS: Our study indicates clearly that ASE treatment caused teratogenic effect only at doses above 240 mg/kg (> 20{\%} of LD50). Lower doses had no developmental toxicity.",
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Induction of Developmental Toxicity in Mice Treated with Alstonia scholaris (Sapthaparna) in Utero. / Jagetia, Ganesh Chandra; Baliga, Manjeshwar Shrinath.

In: Birth Defects Research Part B - Developmental and Reproductive Toxicology, Vol. 68, No. 6, 01.12.2003, p. 472-478.

Research output: Contribution to journalArticle

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