Abstract
Natural medicinal systems such as Ayurveda and folk medicine has remedies for wound management. However, the exact cellular and extracellular mechanisms involved in the healing process and its influence on keratinocytes is less discussed. Therefore, the present study was designed to evaluate the effect of certain natural wound healing medicines on the biology of the keratinocytes/HaCaT cells. Test materials such as honey (H), ghee (G), aqueous extracts of roots of Glycyrrhiza glabra (GG) and leaves of Nerium indicum (NI) were considered. The HaCaT cells were treated with the test materials singly and in combinations (H+G, all combined [Tot]) for a specific period (24, 48, and 72 hours). The cells were then subjected to cytotoxicity/proliferation and migration/scratch assays. All the test materials, except NI, were non-cytotoxic and showed increased cell proliferation at variable concentrations. Significant observations were made in the groups treated with honey (100 μg/ml at 48 hours, P<0.05; 1,000 μg/ml at 72 hours, P<0.05), GG (all concentrations at 48 hours, P<0.05; 750 μg/ml at 72 hours, P<0.05), H+G (250 μg/ml at 24 hours, P<0.001; 500 μg/ml at 48 and 72 hours, P<0.05), and Tot (50 μg/ml at 24, 48 and 72 hours, P<0.01). In the in-vitro wound healing assay, all the treated groups showed significant migration and narrowing of the scratch area by 24 and 48 hours (P<0.001) compared to control. The results obtained from the present study signifies the positive influence of these natural wound healing compounds on keratinocytes/HaCaT cells.
| Original language | English |
|---|---|
| Pages (from-to) | 324-332 |
| Number of pages | 9 |
| Journal | Anatomy and Cell Biology |
| Volume | 52 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - 01-01-2019 |
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All Science Journal Classification (ASJC) codes
- Anatomy
- Histology
- Developmental Biology
- Cellular and Molecular Neuroscience
- Cell Biology
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Influence of traditional medicines on the activity of keratinocytes in wound healing : An in-vitro study. / Kotian, Sushma R.; Bhat, Kumar M.R.; Padma, Divya; Pai, K. Sreedhara R.
In: Anatomy and Cell Biology, Vol. 52, No. 3, 01.01.2019, p. 324-332.Research output: Contribution to journal › Article
TY - JOUR
T1 - Influence of traditional medicines on the activity of keratinocytes in wound healing
T2 - An in-vitro study
AU - Kotian, Sushma R.
AU - Bhat, Kumar M.R.
AU - Padma, Divya
AU - Pai, K. Sreedhara R.
PY - 2019/1/1
Y1 - 2019/1/1
N2 - Natural medicinal systems such as Ayurveda and folk medicine has remedies for wound management. However, the exact cellular and extracellular mechanisms involved in the healing process and its influence on keratinocytes is less discussed. Therefore, the present study was designed to evaluate the effect of certain natural wound healing medicines on the biology of the keratinocytes/HaCaT cells. Test materials such as honey (H), ghee (G), aqueous extracts of roots of Glycyrrhiza glabra (GG) and leaves of Nerium indicum (NI) were considered. The HaCaT cells were treated with the test materials singly and in combinations (H+G, all combined [Tot]) for a specific period (24, 48, and 72 hours). The cells were then subjected to cytotoxicity/proliferation and migration/scratch assays. All the test materials, except NI, were non-cytotoxic and showed increased cell proliferation at variable concentrations. Significant observations were made in the groups treated with honey (100 μg/ml at 48 hours, P<0.05; 1,000 μg/ml at 72 hours, P<0.05), GG (all concentrations at 48 hours, P<0.05; 750 μg/ml at 72 hours, P<0.05), H+G (250 μg/ml at 24 hours, P<0.001; 500 μg/ml at 48 and 72 hours, P<0.05), and Tot (50 μg/ml at 24, 48 and 72 hours, P<0.01). In the in-vitro wound healing assay, all the treated groups showed significant migration and narrowing of the scratch area by 24 and 48 hours (P<0.001) compared to control. The results obtained from the present study signifies the positive influence of these natural wound healing compounds on keratinocytes/HaCaT cells.
AB - Natural medicinal systems such as Ayurveda and folk medicine has remedies for wound management. However, the exact cellular and extracellular mechanisms involved in the healing process and its influence on keratinocytes is less discussed. Therefore, the present study was designed to evaluate the effect of certain natural wound healing medicines on the biology of the keratinocytes/HaCaT cells. Test materials such as honey (H), ghee (G), aqueous extracts of roots of Glycyrrhiza glabra (GG) and leaves of Nerium indicum (NI) were considered. The HaCaT cells were treated with the test materials singly and in combinations (H+G, all combined [Tot]) for a specific period (24, 48, and 72 hours). The cells were then subjected to cytotoxicity/proliferation and migration/scratch assays. All the test materials, except NI, were non-cytotoxic and showed increased cell proliferation at variable concentrations. Significant observations were made in the groups treated with honey (100 μg/ml at 48 hours, P<0.05; 1,000 μg/ml at 72 hours, P<0.05), GG (all concentrations at 48 hours, P<0.05; 750 μg/ml at 72 hours, P<0.05), H+G (250 μg/ml at 24 hours, P<0.001; 500 μg/ml at 48 and 72 hours, P<0.05), and Tot (50 μg/ml at 24, 48 and 72 hours, P<0.01). In the in-vitro wound healing assay, all the treated groups showed significant migration and narrowing of the scratch area by 24 and 48 hours (P<0.001) compared to control. The results obtained from the present study signifies the positive influence of these natural wound healing compounds on keratinocytes/HaCaT cells.
UR - http://www.scopus.com/inward/record.url?scp=85074573241&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85074573241&partnerID=8YFLogxK
U2 - 10.5115/acb.19.009
DO - 10.5115/acb.19.009
M3 - Article
AN - SCOPUS:85074573241
VL - 52
SP - 324
EP - 332
JO - Anatomy and Cell Biology
JF - Anatomy and Cell Biology
SN - 2093-3665
IS - 3
ER -