Inhibition of angiotensin converting enzyme from sheep tissues by captopril, lisinopril and enalapril

E. G.Padmanabha Udupa, N. Mallikarjuna Rao

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Inhibition of angiotensin converting enzyme(EC 3.4.15.1, ACE) in presence of captopril, lisinopril and enalapril were investigated in kidney, lung and serum of sheep using Hip-His-Leu(HHL) as substrate. The activity in kidney, lung and serum was inhibited at HHL concentration above 5 mM. The inhibitory constants (IC50) ranged between 5.6 nM for serum ACE with lisinopril and 70000 nM for renal ACE with enalapril while K1 ranged from 1.0 nM for serum ACE with lisinopril to 12000 nM for kidney ACE with enalapril. Differences in inhibition observed in different tissues suggest that the inhibitors may block function(s) of ACE to varying degrees in each tissue.

Original languageEnglish
Pages (from-to)524-528
Number of pages5
JournalIndian Journal of Biochemistry and Biophysics
Volume34
Issue number6
Publication statusPublished - 01-12-1997

Fingerprint

Lisinopril
Enalapril
Captopril
Peptidyl-Dipeptidase A
Sheep
Tissue
Kidney
Serum
Lung
Inhibitory Concentration 50
Substrates
hippuryl-histidyl-leucine

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry

Cite this

@article{4db3b91dc0174d15bb45c3cd2364e898,
title = "Inhibition of angiotensin converting enzyme from sheep tissues by captopril, lisinopril and enalapril",
abstract = "Inhibition of angiotensin converting enzyme(EC 3.4.15.1, ACE) in presence of captopril, lisinopril and enalapril were investigated in kidney, lung and serum of sheep using Hip-His-Leu(HHL) as substrate. The activity in kidney, lung and serum was inhibited at HHL concentration above 5 mM. The inhibitory constants (IC50) ranged between 5.6 nM for serum ACE with lisinopril and 70000 nM for renal ACE with enalapril while K1 ranged from 1.0 nM for serum ACE with lisinopril to 12000 nM for kidney ACE with enalapril. Differences in inhibition observed in different tissues suggest that the inhibitors may block function(s) of ACE to varying degrees in each tissue.",
author = "Udupa, {E. G.Padmanabha} and Rao, {N. Mallikarjuna}",
year = "1997",
month = "12",
day = "1",
language = "English",
volume = "34",
pages = "524--528",
journal = "Indian Journal of Biochemistry and Biophysics",
issn = "0301-1208",
publisher = "National Institute of Science Communication and Information Resources (NISCAIR)",
number = "6",

}

Inhibition of angiotensin converting enzyme from sheep tissues by captopril, lisinopril and enalapril. / Udupa, E. G.Padmanabha; Rao, N. Mallikarjuna.

In: Indian Journal of Biochemistry and Biophysics, Vol. 34, No. 6, 01.12.1997, p. 524-528.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Inhibition of angiotensin converting enzyme from sheep tissues by captopril, lisinopril and enalapril

AU - Udupa, E. G.Padmanabha

AU - Rao, N. Mallikarjuna

PY - 1997/12/1

Y1 - 1997/12/1

N2 - Inhibition of angiotensin converting enzyme(EC 3.4.15.1, ACE) in presence of captopril, lisinopril and enalapril were investigated in kidney, lung and serum of sheep using Hip-His-Leu(HHL) as substrate. The activity in kidney, lung and serum was inhibited at HHL concentration above 5 mM. The inhibitory constants (IC50) ranged between 5.6 nM for serum ACE with lisinopril and 70000 nM for renal ACE with enalapril while K1 ranged from 1.0 nM for serum ACE with lisinopril to 12000 nM for kidney ACE with enalapril. Differences in inhibition observed in different tissues suggest that the inhibitors may block function(s) of ACE to varying degrees in each tissue.

AB - Inhibition of angiotensin converting enzyme(EC 3.4.15.1, ACE) in presence of captopril, lisinopril and enalapril were investigated in kidney, lung and serum of sheep using Hip-His-Leu(HHL) as substrate. The activity in kidney, lung and serum was inhibited at HHL concentration above 5 mM. The inhibitory constants (IC50) ranged between 5.6 nM for serum ACE with lisinopril and 70000 nM for renal ACE with enalapril while K1 ranged from 1.0 nM for serum ACE with lisinopril to 12000 nM for kidney ACE with enalapril. Differences in inhibition observed in different tissues suggest that the inhibitors may block function(s) of ACE to varying degrees in each tissue.

UR - http://www.scopus.com/inward/record.url?scp=0031310779&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031310779&partnerID=8YFLogxK

M3 - Article

VL - 34

SP - 524

EP - 528

JO - Indian Journal of Biochemistry and Biophysics

JF - Indian Journal of Biochemistry and Biophysics

SN - 0301-1208

IS - 6

ER -