Inhibition of Radiation-Induced Lipid Peroxidation by Tetrahydrocurcumin: Possible Mechanisms by Pulse Radiolysis

S.M. Khopde, K.I. Priyadarsini, S.N. Guha, J.G. Satav, P. Venkatesan, M.N.A. Rao

Research output: Contribution to journalArticle

81 Citations (Scopus)

Abstract

The antioxidant property of tetrahydrocurcumin (THC), a reduced derivative of curcumin, was examined by its ability to inhibit radiation-induced lipid peroxidation in rat liver microsomes and compared with curcumin. The lipid peroxidation caused by irradiation of N2O-purged and aerated buffered aqueous solutions was found to be inhibited by THC in a dose- and concentration-dependent manner. In order to understand the actual reaction mechanisms involved in the inhibition process, pulse radiolysis investigation of THC with radiolytically produced radicals like hydroxyl, model peroxyl radicals, and azide radicals were done and the transients were detected by kinetic spectrophotometry. The reaction of THC with hydroxyl and azide radicals gave rise to transient absorption in the region 200-400 nm with two peaks at 310 nm and 390 nm. From the spectral properties and kinetics of these radicals, a suitable mechanism is discussed to explain the antioxidant actions of THC. © 2000 by Japan Society for Bioscience, Biotechnology, and Agrochemistry.
Original languageUndefined/Unknown
Pages (from-to)503-509
Number of pages7
JournalBioscience, Biotechnology and Biochemistry
Volume64
Issue number3
DOIs
Publication statusPublished - 2000

Cite this

Khopde, S.M. ; Priyadarsini, K.I. ; Guha, S.N. ; Satav, J.G. ; Venkatesan, P. ; Rao, M.N.A. / Inhibition of Radiation-Induced Lipid Peroxidation by Tetrahydrocurcumin: Possible Mechanisms by Pulse Radiolysis. In: Bioscience, Biotechnology and Biochemistry. 2000 ; Vol. 64, No. 3. pp. 503-509.
@article{9b0aabff890c40cfbd95c63bff9cb154,
title = "Inhibition of Radiation-Induced Lipid Peroxidation by Tetrahydrocurcumin: Possible Mechanisms by Pulse Radiolysis",
abstract = "The antioxidant property of tetrahydrocurcumin (THC), a reduced derivative of curcumin, was examined by its ability to inhibit radiation-induced lipid peroxidation in rat liver microsomes and compared with curcumin. The lipid peroxidation caused by irradiation of N2O-purged and aerated buffered aqueous solutions was found to be inhibited by THC in a dose- and concentration-dependent manner. In order to understand the actual reaction mechanisms involved in the inhibition process, pulse radiolysis investigation of THC with radiolytically produced radicals like hydroxyl, model peroxyl radicals, and azide radicals were done and the transients were detected by kinetic spectrophotometry. The reaction of THC with hydroxyl and azide radicals gave rise to transient absorption in the region 200-400 nm with two peaks at 310 nm and 390 nm. From the spectral properties and kinetics of these radicals, a suitable mechanism is discussed to explain the antioxidant actions of THC. {\circledC} 2000 by Japan Society for Bioscience, Biotechnology, and Agrochemistry.",
author = "S.M. Khopde and K.I. Priyadarsini and S.N. Guha and J.G. Satav and P. Venkatesan and M.N.A. Rao",
note = "cited By 70",
year = "2000",
doi = "10.1271/bbb.64.503",
language = "Undefined/Unknown",
volume = "64",
pages = "503--509",
journal = "Bioscience, Biotechnology and Biochemistry",
issn = "0916-8451",
publisher = "Japan Society for Bioscience Biotechnology and Agrochemistry",
number = "3",

}

Inhibition of Radiation-Induced Lipid Peroxidation by Tetrahydrocurcumin: Possible Mechanisms by Pulse Radiolysis. / Khopde, S.M.; Priyadarsini, K.I.; Guha, S.N.; Satav, J.G.; Venkatesan, P.; Rao, M.N.A.

In: Bioscience, Biotechnology and Biochemistry, Vol. 64, No. 3, 2000, p. 503-509.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Inhibition of Radiation-Induced Lipid Peroxidation by Tetrahydrocurcumin: Possible Mechanisms by Pulse Radiolysis

AU - Khopde, S.M.

AU - Priyadarsini, K.I.

AU - Guha, S.N.

AU - Satav, J.G.

AU - Venkatesan, P.

AU - Rao, M.N.A.

N1 - cited By 70

PY - 2000

Y1 - 2000

N2 - The antioxidant property of tetrahydrocurcumin (THC), a reduced derivative of curcumin, was examined by its ability to inhibit radiation-induced lipid peroxidation in rat liver microsomes and compared with curcumin. The lipid peroxidation caused by irradiation of N2O-purged and aerated buffered aqueous solutions was found to be inhibited by THC in a dose- and concentration-dependent manner. In order to understand the actual reaction mechanisms involved in the inhibition process, pulse radiolysis investigation of THC with radiolytically produced radicals like hydroxyl, model peroxyl radicals, and azide radicals were done and the transients were detected by kinetic spectrophotometry. The reaction of THC with hydroxyl and azide radicals gave rise to transient absorption in the region 200-400 nm with two peaks at 310 nm and 390 nm. From the spectral properties and kinetics of these radicals, a suitable mechanism is discussed to explain the antioxidant actions of THC. © 2000 by Japan Society for Bioscience, Biotechnology, and Agrochemistry.

AB - The antioxidant property of tetrahydrocurcumin (THC), a reduced derivative of curcumin, was examined by its ability to inhibit radiation-induced lipid peroxidation in rat liver microsomes and compared with curcumin. The lipid peroxidation caused by irradiation of N2O-purged and aerated buffered aqueous solutions was found to be inhibited by THC in a dose- and concentration-dependent manner. In order to understand the actual reaction mechanisms involved in the inhibition process, pulse radiolysis investigation of THC with radiolytically produced radicals like hydroxyl, model peroxyl radicals, and azide radicals were done and the transients were detected by kinetic spectrophotometry. The reaction of THC with hydroxyl and azide radicals gave rise to transient absorption in the region 200-400 nm with two peaks at 310 nm and 390 nm. From the spectral properties and kinetics of these radicals, a suitable mechanism is discussed to explain the antioxidant actions of THC. © 2000 by Japan Society for Bioscience, Biotechnology, and Agrochemistry.

U2 - 10.1271/bbb.64.503

DO - 10.1271/bbb.64.503

M3 - Article

VL - 64

SP - 503

EP - 509

JO - Bioscience, Biotechnology and Biochemistry

JF - Bioscience, Biotechnology and Biochemistry

SN - 0916-8451

IS - 3

ER -