Insulin blocks glutamate-induced neurotoxicity in differentiated SH-SY5Y neuronal cells

Madhavan Nampoothiri, Neetinkumar D. Reddy, Jessy John, Nitesh Kumar, Gopalan Kutty Nampurath, Mallikarjuna Rao Chamallamudi

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Insulin is a cytokine which promotes cell growth. Recently, a few published reports on insulin in different cell lines support the antiapoptotic effect of insulin. But the reports fail to explain the role of insulin in modulating glutamate-mediated neuronal cell death through excitotoxicity. Thus, we examined the neuroprotective effect of insulin on glutamate-induced toxicity on differentiated SH-SY5Y neuronal cells. Changes in cell viability were measured by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) based assay, while apoptotic damage was detected by acridine orange/ethidium bromide and Hoechst staining. Intracellular reactive oxygen species (ROS) accumulation and morphological alterations were also measured. Treatment with glutamate induced apoptosis, elevated ROS levels and caused damage to neurons. Insulin was able to attenuate the glutamate-induced excitotoxic damage to neuronal cells.

Original languageEnglish
Article number674164
JournalBehavioural Neurology
Volume2014
DOIs
Publication statusPublished - 2014

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Glutamic Acid
Insulin
Reactive Oxygen Species
Acridine Orange
Ethidium
Neuroprotective Agents
Cell Survival
Cell Death
Apoptosis
Staining and Labeling
Cytokines
Neurons
Cell Line
Growth

All Science Journal Classification (ASJC) codes

  • Neuropsychology and Physiological Psychology
  • Neurology
  • Clinical Neurology

Cite this

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abstract = "Insulin is a cytokine which promotes cell growth. Recently, a few published reports on insulin in different cell lines support the antiapoptotic effect of insulin. But the reports fail to explain the role of insulin in modulating glutamate-mediated neuronal cell death through excitotoxicity. Thus, we examined the neuroprotective effect of insulin on glutamate-induced toxicity on differentiated SH-SY5Y neuronal cells. Changes in cell viability were measured by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) based assay, while apoptotic damage was detected by acridine orange/ethidium bromide and Hoechst staining. Intracellular reactive oxygen species (ROS) accumulation and morphological alterations were also measured. Treatment with glutamate induced apoptosis, elevated ROS levels and caused damage to neurons. Insulin was able to attenuate the glutamate-induced excitotoxic damage to neuronal cells.",
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Insulin blocks glutamate-induced neurotoxicity in differentiated SH-SY5Y neuronal cells. / Nampoothiri, Madhavan; Reddy, Neetinkumar D.; John, Jessy; Kumar, Nitesh; Kutty Nampurath, Gopalan; Rao Chamallamudi, Mallikarjuna.

In: Behavioural Neurology, Vol. 2014, 674164, 2014.

Research output: Contribution to journalArticle

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AU - Nampoothiri, Madhavan

AU - Reddy, Neetinkumar D.

AU - John, Jessy

AU - Kumar, Nitesh

AU - Kutty Nampurath, Gopalan

AU - Rao Chamallamudi, Mallikarjuna

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