Insulin protects against brain oxidative stress with an apparent effect on episodic memory in doxorubicin-induced cognitive dysfunction in wistar rats

Grandhi Venkata Ramalingayya, Vishwajeet Sonawane, Sri Pragnya Cheruku, Anoop Kishore, Pawan G. Nayak, Nitesh Kumar, Rekha R. Shenoy, Krishnadas Nandakumar

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The present study was aimed at assessing the protective effect of insulin against doxorubicin (DOX) -induced cognitive dysfunction in Wistar rats. Cognitive function for episodic memory was assessed by a novel object recognition task (NORT) in male Wistar rats. Oxidative stress markers—SOD, catalase, glutathione, and lipid peroxidation—in the hippocampus and frontal cortex were assessed using colorimetric methods. Doxorubicin treatment (2.5 mg/kg, i.p., every 5 days for 50 days) reduced recognition and discriminative indices in NORT with increased oxidative stress in the brain. A nonhypoglycemic dose of insulin (0.5 IU/kg, i.p.) significantly reduced brain oxidative stress (MDA) induced by doxorubicin with an increase in the antioxidant defense systems (SOD, catalase, and GSH). Rats treated with combined insulin and DOX spent comparatively more time with the novel object when compared to the non-novel objects; however, the observed difference was not statistically significant. An apparent improvement (p < 0.26) in recognition of the novel object was observed against the damage induced by doxorubicin. These results suggest that insulin reduces brain oxidative stress and apparently improves doxorubicin-induced cognitive dysfunction in Wistar rats.

Original languageEnglish
Pages (from-to)121-130
Number of pages10
JournalJournal of Environmental Pathology, Toxicology and Oncology
Volume36
Issue number2
Publication statusPublished - 2017

Fingerprint

Oxidative stress
Episodic Memory
Doxorubicin
Wistar Rats
Rats
Brain
Oxidative Stress
Insulin
Data storage equipment
Object recognition
Catalase
Frontal Lobe
Cognition
Glutathione
Cognitive Dysfunction
Hippocampus
Antioxidants
Lipids
Recognition (Psychology)

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine
  • Medicine(all)
  • Toxicology
  • Health, Toxicology and Mutagenesis

Cite this

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title = "Insulin protects against brain oxidative stress with an apparent effect on episodic memory in doxorubicin-induced cognitive dysfunction in wistar rats",
abstract = "The present study was aimed at assessing the protective effect of insulin against doxorubicin (DOX) -induced cognitive dysfunction in Wistar rats. Cognitive function for episodic memory was assessed by a novel object recognition task (NORT) in male Wistar rats. Oxidative stress markers—SOD, catalase, glutathione, and lipid peroxidation—in the hippocampus and frontal cortex were assessed using colorimetric methods. Doxorubicin treatment (2.5 mg/kg, i.p., every 5 days for 50 days) reduced recognition and discriminative indices in NORT with increased oxidative stress in the brain. A nonhypoglycemic dose of insulin (0.5 IU/kg, i.p.) significantly reduced brain oxidative stress (MDA) induced by doxorubicin with an increase in the antioxidant defense systems (SOD, catalase, and GSH). Rats treated with combined insulin and DOX spent comparatively more time with the novel object when compared to the non-novel objects; however, the observed difference was not statistically significant. An apparent improvement (p < 0.26) in recognition of the novel object was observed against the damage induced by doxorubicin. These results suggest that insulin reduces brain oxidative stress and apparently improves doxorubicin-induced cognitive dysfunction in Wistar rats.",
author = "Ramalingayya, {Grandhi Venkata} and Vishwajeet Sonawane and Cheruku, {Sri Pragnya} and Anoop Kishore and Nayak, {Pawan G.} and Nitesh Kumar and Shenoy, {Rekha R.} and Krishnadas Nandakumar",
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AU - Ramalingayya, Grandhi Venkata

AU - Sonawane, Vishwajeet

AU - Cheruku, Sri Pragnya

AU - Kishore, Anoop

AU - Nayak, Pawan G.

AU - Kumar, Nitesh

AU - Shenoy, Rekha R.

AU - Nandakumar, Krishnadas

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