Investigation of Calpain 10 (rs2975760) gene polymorphism in Asian Indians with Gestational Diabetes Mellitus

Imran Ali Khan, Sireesha Movva, Noor Ahmad Shaik, Srinivas Chava, Parveen Jahan, Kamal Kiran Mukkavali, Vasundhara Kamineni, Qurratulain Hasan, Pragna Rao

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Background: Type 2 Diabetes Mellitus (T2DM) and Gestational Diabetes Mellitus (GDM) are part of a heterogeneous and complex metabolic group of disorders that share common pathophysiological circumstances, including β-cell dysfunction and insulin resistance. The protein Calpain 10 (CAPN10) plays a role in glucose metabolism, pancreatic β-cell insulin secretion, and thermogenesis. Objective: Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) based genotyping of CAPN10 (rs2975760) polymorphism was carried out in T2DM and GDM with suitable controls for each of the pathologies fromthe same population. Genomic DNA was isolated from 787 participants, including 250 cases of T2DM, 287 pregnant women, of which 137 were identified as having GDM and the remaining 150 were confirmed as non-GDM, and 250 healthy control volunteers, and association analysis was carried out for genotypes and alleles. Results: In the present study, T2DM was compared with healthy controls and was not found to be associated with the CAPN10 C allele (odds ratio, OR: 1.09; 95% CI = 0.8011-1.484; p = 0.5821). GDM also did not show any association when compared with non-GDM (OR: 1.124; 95% CI = 0.7585-1.667; p = 0.5606) respectively. ConclusionOur study suggests that the CAPN10 (rs2975760) polymorphism scrutinized in this study is not associated with T2DM and GDM.

Original languageEnglish
Pages (from-to)299-306
Number of pages8
JournalMeta Gene
Volume2
Issue number1
DOIs
Publication statusPublished - 2014

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Gestational Diabetes
Type 2 Diabetes Mellitus
Genes
Diabetes Mellitus
Alleles
Thermogenesis
Restriction Fragment Length Polymorphisms
Insulin Resistance
Pregnant Women
Healthy Volunteers
Odds Ratio
Genotype
calpain 10
Insulin
Pathology
Glucose
Polymerase Chain Reaction
DNA
Population
Proteins

All Science Journal Classification (ASJC) codes

  • Genetics
  • Genetics(clinical)

Cite this

Khan, Imran Ali ; Movva, Sireesha ; Shaik, Noor Ahmad ; Chava, Srinivas ; Jahan, Parveen ; Mukkavali, Kamal Kiran ; Kamineni, Vasundhara ; Hasan, Qurratulain ; Rao, Pragna. / Investigation of Calpain 10 (rs2975760) gene polymorphism in Asian Indians with Gestational Diabetes Mellitus. In: Meta Gene. 2014 ; Vol. 2, No. 1. pp. 299-306.
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abstract = "Background: Type 2 Diabetes Mellitus (T2DM) and Gestational Diabetes Mellitus (GDM) are part of a heterogeneous and complex metabolic group of disorders that share common pathophysiological circumstances, including β-cell dysfunction and insulin resistance. The protein Calpain 10 (CAPN10) plays a role in glucose metabolism, pancreatic β-cell insulin secretion, and thermogenesis. Objective: Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) based genotyping of CAPN10 (rs2975760) polymorphism was carried out in T2DM and GDM with suitable controls for each of the pathologies fromthe same population. Genomic DNA was isolated from 787 participants, including 250 cases of T2DM, 287 pregnant women, of which 137 were identified as having GDM and the remaining 150 were confirmed as non-GDM, and 250 healthy control volunteers, and association analysis was carried out for genotypes and alleles. Results: In the present study, T2DM was compared with healthy controls and was not found to be associated with the CAPN10 C allele (odds ratio, OR: 1.09; 95{\%} CI = 0.8011-1.484; p = 0.5821). GDM also did not show any association when compared with non-GDM (OR: 1.124; 95{\%} CI = 0.7585-1.667; p = 0.5606) respectively. ConclusionOur study suggests that the CAPN10 (rs2975760) polymorphism scrutinized in this study is not associated with T2DM and GDM.",
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Khan, IA, Movva, S, Shaik, NA, Chava, S, Jahan, P, Mukkavali, KK, Kamineni, V, Hasan, Q & Rao, P 2014, 'Investigation of Calpain 10 (rs2975760) gene polymorphism in Asian Indians with Gestational Diabetes Mellitus', Meta Gene, vol. 2, no. 1, pp. 299-306. https://doi.org/10.1016/j.mgene.2014.03.001

Investigation of Calpain 10 (rs2975760) gene polymorphism in Asian Indians with Gestational Diabetes Mellitus. / Khan, Imran Ali; Movva, Sireesha; Shaik, Noor Ahmad; Chava, Srinivas; Jahan, Parveen; Mukkavali, Kamal Kiran; Kamineni, Vasundhara; Hasan, Qurratulain; Rao, Pragna.

In: Meta Gene, Vol. 2, No. 1, 2014, p. 299-306.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Investigation of Calpain 10 (rs2975760) gene polymorphism in Asian Indians with Gestational Diabetes Mellitus

AU - Khan, Imran Ali

AU - Movva, Sireesha

AU - Shaik, Noor Ahmad

AU - Chava, Srinivas

AU - Jahan, Parveen

AU - Mukkavali, Kamal Kiran

AU - Kamineni, Vasundhara

AU - Hasan, Qurratulain

AU - Rao, Pragna

PY - 2014

Y1 - 2014

N2 - Background: Type 2 Diabetes Mellitus (T2DM) and Gestational Diabetes Mellitus (GDM) are part of a heterogeneous and complex metabolic group of disorders that share common pathophysiological circumstances, including β-cell dysfunction and insulin resistance. The protein Calpain 10 (CAPN10) plays a role in glucose metabolism, pancreatic β-cell insulin secretion, and thermogenesis. Objective: Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) based genotyping of CAPN10 (rs2975760) polymorphism was carried out in T2DM and GDM with suitable controls for each of the pathologies fromthe same population. Genomic DNA was isolated from 787 participants, including 250 cases of T2DM, 287 pregnant women, of which 137 were identified as having GDM and the remaining 150 were confirmed as non-GDM, and 250 healthy control volunteers, and association analysis was carried out for genotypes and alleles. Results: In the present study, T2DM was compared with healthy controls and was not found to be associated with the CAPN10 C allele (odds ratio, OR: 1.09; 95% CI = 0.8011-1.484; p = 0.5821). GDM also did not show any association when compared with non-GDM (OR: 1.124; 95% CI = 0.7585-1.667; p = 0.5606) respectively. ConclusionOur study suggests that the CAPN10 (rs2975760) polymorphism scrutinized in this study is not associated with T2DM and GDM.

AB - Background: Type 2 Diabetes Mellitus (T2DM) and Gestational Diabetes Mellitus (GDM) are part of a heterogeneous and complex metabolic group of disorders that share common pathophysiological circumstances, including β-cell dysfunction and insulin resistance. The protein Calpain 10 (CAPN10) plays a role in glucose metabolism, pancreatic β-cell insulin secretion, and thermogenesis. Objective: Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) based genotyping of CAPN10 (rs2975760) polymorphism was carried out in T2DM and GDM with suitable controls for each of the pathologies fromthe same population. Genomic DNA was isolated from 787 participants, including 250 cases of T2DM, 287 pregnant women, of which 137 were identified as having GDM and the remaining 150 were confirmed as non-GDM, and 250 healthy control volunteers, and association analysis was carried out for genotypes and alleles. Results: In the present study, T2DM was compared with healthy controls and was not found to be associated with the CAPN10 C allele (odds ratio, OR: 1.09; 95% CI = 0.8011-1.484; p = 0.5821). GDM also did not show any association when compared with non-GDM (OR: 1.124; 95% CI = 0.7585-1.667; p = 0.5606) respectively. ConclusionOur study suggests that the CAPN10 (rs2975760) polymorphism scrutinized in this study is not associated with T2DM and GDM.

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