Involvement of oxidative and nitrosative stress in modulation of gene expression and functional responses by IFNγ

S. Jyothi Prasanna, Banishree Saha, Dipankar Nandi

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

IFNγ is a potent immunomodulator which plays important roles in host defense. IFNγ modulates transcription of growth-related genes [N-myc downstream regulator 1, g rowth arrest and DNA damage inducible γ and inhibitor of DNA binding 2 (Id2)], which is followed by increased growth suppression in the mouse hepatoma cell line, H6. Further studies revealed modulation of genes involved in oxidative and nitrosative stress (iNos, gp91phox and Catalase) and increased generation of reactive oxygen species (ROS) and reactive nitrogen intermediates (RNIs) upon IFNγ treatment. High amounts of ROS and RNI are responsible for IFNγ-mediated reduction in cell growth as this process is blocked, using either diphenylene iodonium (DPI), an inhibitor of flavin-containing NADPH oxidases, or N-methyl L-arginine (LNMA), an inhibitor of nitric oxide synthase. Based on studies with LNMA and DPI, IFNγ-modulated genes can be categorized into two distinct sets: oxidative and nitrosative stress independent (transporter associated with antigen processing 2, Cd80, Lmp10 and Icosl) and oxidative and nitrosative stress dependent (iNos, gp91phox, Catalase and Id2). In addition, DPI or LNMA blocked IFNγ-induced activation of Ras, demonstrating the involvement of oxidative and nitrosative stress. Manumycin A, a farnesyl transferase inhibitor, blocked Ras activation and reduced NADPH oxidase activity and ROS amounts leading to increased cell growth in the presence of IFNγ. Notably, the IFNγ-induced MHC class I levels are not modulated in cells treated with DPI, LNMA or manumycin A. Together, these results delineate the role of high amounts of ROS, RNI and Ras activation in modulating expression of some genes and, thereby, function by IFNγ. The implications of these results during modulation of immune responses by IFNγ are discussed.

Original languageEnglish
Pages (from-to)867-879
Number of pages13
JournalInternational Immunology
Volume19
Issue number7
DOIs
Publication statusPublished - 07-2007

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Reactive Oxygen Species
Oxidative Stress
Gene Expression
Nitrogen
NADPH Oxidase
Catalase
Cell Growth Processes
Growth
myc Genes
DNA
Antigen Presentation
Immunologic Factors
Transferases
Nitric Oxide Synthase
Genes
DNA Damage
Arginine
Hepatocellular Carcinoma
Cell Line
diphenyleneiodonium

All Science Journal Classification (ASJC) codes

  • Immunology

Cite this

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title = "Involvement of oxidative and nitrosative stress in modulation of gene expression and functional responses by IFNγ",
abstract = "IFNγ is a potent immunomodulator which plays important roles in host defense. IFNγ modulates transcription of growth-related genes [N-myc downstream regulator 1, g rowth arrest and DNA damage inducible γ and inhibitor of DNA binding 2 (Id2)], which is followed by increased growth suppression in the mouse hepatoma cell line, H6. Further studies revealed modulation of genes involved in oxidative and nitrosative stress (iNos, gp91phox and Catalase) and increased generation of reactive oxygen species (ROS) and reactive nitrogen intermediates (RNIs) upon IFNγ treatment. High amounts of ROS and RNI are responsible for IFNγ-mediated reduction in cell growth as this process is blocked, using either diphenylene iodonium (DPI), an inhibitor of flavin-containing NADPH oxidases, or N-methyl L-arginine (LNMA), an inhibitor of nitric oxide synthase. Based on studies with LNMA and DPI, IFNγ-modulated genes can be categorized into two distinct sets: oxidative and nitrosative stress independent (transporter associated with antigen processing 2, Cd80, Lmp10 and Icosl) and oxidative and nitrosative stress dependent (iNos, gp91phox, Catalase and Id2). In addition, DPI or LNMA blocked IFNγ-induced activation of Ras, demonstrating the involvement of oxidative and nitrosative stress. Manumycin A, a farnesyl transferase inhibitor, blocked Ras activation and reduced NADPH oxidase activity and ROS amounts leading to increased cell growth in the presence of IFNγ. Notably, the IFNγ-induced MHC class I levels are not modulated in cells treated with DPI, LNMA or manumycin A. Together, these results delineate the role of high amounts of ROS, RNI and Ras activation in modulating expression of some genes and, thereby, function by IFNγ. The implications of these results during modulation of immune responses by IFNγ are discussed.",
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Involvement of oxidative and nitrosative stress in modulation of gene expression and functional responses by IFNγ. / Prasanna, S. Jyothi; Saha, Banishree; Nandi, Dipankar.

In: International Immunology, Vol. 19, No. 7, 07.2007, p. 867-879.

Research output: Contribution to journalArticle

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AB - IFNγ is a potent immunomodulator which plays important roles in host defense. IFNγ modulates transcription of growth-related genes [N-myc downstream regulator 1, g rowth arrest and DNA damage inducible γ and inhibitor of DNA binding 2 (Id2)], which is followed by increased growth suppression in the mouse hepatoma cell line, H6. Further studies revealed modulation of genes involved in oxidative and nitrosative stress (iNos, gp91phox and Catalase) and increased generation of reactive oxygen species (ROS) and reactive nitrogen intermediates (RNIs) upon IFNγ treatment. High amounts of ROS and RNI are responsible for IFNγ-mediated reduction in cell growth as this process is blocked, using either diphenylene iodonium (DPI), an inhibitor of flavin-containing NADPH oxidases, or N-methyl L-arginine (LNMA), an inhibitor of nitric oxide synthase. Based on studies with LNMA and DPI, IFNγ-modulated genes can be categorized into two distinct sets: oxidative and nitrosative stress independent (transporter associated with antigen processing 2, Cd80, Lmp10 and Icosl) and oxidative and nitrosative stress dependent (iNos, gp91phox, Catalase and Id2). In addition, DPI or LNMA blocked IFNγ-induced activation of Ras, demonstrating the involvement of oxidative and nitrosative stress. Manumycin A, a farnesyl transferase inhibitor, blocked Ras activation and reduced NADPH oxidase activity and ROS amounts leading to increased cell growth in the presence of IFNγ. Notably, the IFNγ-induced MHC class I levels are not modulated in cells treated with DPI, LNMA or manumycin A. Together, these results delineate the role of high amounts of ROS, RNI and Ras activation in modulating expression of some genes and, thereby, function by IFNγ. The implications of these results during modulation of immune responses by IFNγ are discussed.

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