IPEB transcription factor regulating the intracisternal a particle gene during F9 cell differentiation is expressed at sites of lymphoid development

Jaya P. Kamat, Amitabha Basu, Kapaettu Satyamoorthy, Mei Q. Xu, Louise Showe, Chin C. Howe

    Research output: Contribution to journalArticle

    3 Citations (Scopus)

    Abstract

    The murine intracisternal A particle (IAP) proviral elements are expressed at low levels in undifferentiated F9 embryonal carcinoma cells but are highly expressed when F9 cells are induced to differentiate into parietal endoderm‐like cells. IAP elements are also expressed in parietal endoderm‐like PYS‐2 cells. We previously identified an IAP proximal enhancer (IPE) element that mediates a F9 differentiation‐specific enhancer activity. We also identified a 60 kDa IPE binding (IPEB) protein whose activity is high in PYS‐2 cells, where IAP is expressed, but very low in F9 cells. Transcription of IAP elements has also been shown in the adult mouse thymus and in activated splenic B cells. We have now shown by DNA affinity chromatography, sodium dodecyl sulfate‐polyacrylamide gel electrophoresis, and band‐shift analysis that the 60 kDa IPEB is expressed in adult T lymphocytes and in resting as well as lipopolysaccharide activated splenic B cells but not in adult liver cells, suggesting an important role for IPEB in IAP transcription in vivo. In addition, we find IPEB expressed in the fetal mouse at sites of lymphoid development, such as the liver, spleen, and thymus, suggesting it may play an important role in gene expression during lymphoid development. In support of this, we find IPEB in the human T cell tumor lines, Jurkat and Molt 13, as well as the Daudi B cell line and in the normal calf thymus and in the thymus and spleen of the chicken and rat. © 1995 Wiley‐Liss, Inc.

    Original languageEnglish
    Pages (from-to)8-15
    Number of pages8
    JournalMolecular Reproduction and Development
    Volume41
    Issue number1
    DOIs
    Publication statusPublished - 1995

    Fingerprint

    Intracisternal A-Particle Genes
    Cell Differentiation
    Transcription Factors
    Thymus Gland
    Genes
    B-Lymphocytes
    Spleen
    T-Lymphocytes
    Embryonal Carcinoma Stem Cells
    Cell Line
    Liver
    Affinity Chromatography
    Lipopolysaccharides
    Electrophoresis
    Chickens
    Carrier Proteins
    Gels
    Sodium
    Gene Expression
    DNA

    All Science Journal Classification (ASJC) codes

    • Genetics
    • Developmental Biology
    • Cell Biology

    Cite this

    @article{e63b511d14b34e94a22e13c88d25a74a,
    title = "IPEB transcription factor regulating the intracisternal a particle gene during F9 cell differentiation is expressed at sites of lymphoid development",
    abstract = "The murine intracisternal A particle (IAP) proviral elements are expressed at low levels in undifferentiated F9 embryonal carcinoma cells but are highly expressed when F9 cells are induced to differentiate into parietal endoderm‐like cells. IAP elements are also expressed in parietal endoderm‐like PYS‐2 cells. We previously identified an IAP proximal enhancer (IPE) element that mediates a F9 differentiation‐specific enhancer activity. We also identified a 60 kDa IPE binding (IPEB) protein whose activity is high in PYS‐2 cells, where IAP is expressed, but very low in F9 cells. Transcription of IAP elements has also been shown in the adult mouse thymus and in activated splenic B cells. We have now shown by DNA affinity chromatography, sodium dodecyl sulfate‐polyacrylamide gel electrophoresis, and band‐shift analysis that the 60 kDa IPEB is expressed in adult T lymphocytes and in resting as well as lipopolysaccharide activated splenic B cells but not in adult liver cells, suggesting an important role for IPEB in IAP transcription in vivo. In addition, we find IPEB expressed in the fetal mouse at sites of lymphoid development, such as the liver, spleen, and thymus, suggesting it may play an important role in gene expression during lymphoid development. In support of this, we find IPEB in the human T cell tumor lines, Jurkat and Molt 13, as well as the Daudi B cell line and in the normal calf thymus and in the thymus and spleen of the chicken and rat. {\circledC} 1995 Wiley‐Liss, Inc.",
    author = "Kamat, {Jaya P.} and Amitabha Basu and Kapaettu Satyamoorthy and Xu, {Mei Q.} and Louise Showe and Howe, {Chin C.}",
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    language = "English",
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    IPEB transcription factor regulating the intracisternal a particle gene during F9 cell differentiation is expressed at sites of lymphoid development. / Kamat, Jaya P.; Basu, Amitabha; Satyamoorthy, Kapaettu; Xu, Mei Q.; Showe, Louise; Howe, Chin C.

    In: Molecular Reproduction and Development, Vol. 41, No. 1, 1995, p. 8-15.

    Research output: Contribution to journalArticle

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    T1 - IPEB transcription factor regulating the intracisternal a particle gene during F9 cell differentiation is expressed at sites of lymphoid development

    AU - Kamat, Jaya P.

    AU - Basu, Amitabha

    AU - Satyamoorthy, Kapaettu

    AU - Xu, Mei Q.

    AU - Showe, Louise

    AU - Howe, Chin C.

    PY - 1995

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    N2 - The murine intracisternal A particle (IAP) proviral elements are expressed at low levels in undifferentiated F9 embryonal carcinoma cells but are highly expressed when F9 cells are induced to differentiate into parietal endoderm‐like cells. IAP elements are also expressed in parietal endoderm‐like PYS‐2 cells. We previously identified an IAP proximal enhancer (IPE) element that mediates a F9 differentiation‐specific enhancer activity. We also identified a 60 kDa IPE binding (IPEB) protein whose activity is high in PYS‐2 cells, where IAP is expressed, but very low in F9 cells. Transcription of IAP elements has also been shown in the adult mouse thymus and in activated splenic B cells. We have now shown by DNA affinity chromatography, sodium dodecyl sulfate‐polyacrylamide gel electrophoresis, and band‐shift analysis that the 60 kDa IPEB is expressed in adult T lymphocytes and in resting as well as lipopolysaccharide activated splenic B cells but not in adult liver cells, suggesting an important role for IPEB in IAP transcription in vivo. In addition, we find IPEB expressed in the fetal mouse at sites of lymphoid development, such as the liver, spleen, and thymus, suggesting it may play an important role in gene expression during lymphoid development. In support of this, we find IPEB in the human T cell tumor lines, Jurkat and Molt 13, as well as the Daudi B cell line and in the normal calf thymus and in the thymus and spleen of the chicken and rat. © 1995 Wiley‐Liss, Inc.

    AB - The murine intracisternal A particle (IAP) proviral elements are expressed at low levels in undifferentiated F9 embryonal carcinoma cells but are highly expressed when F9 cells are induced to differentiate into parietal endoderm‐like cells. IAP elements are also expressed in parietal endoderm‐like PYS‐2 cells. We previously identified an IAP proximal enhancer (IPE) element that mediates a F9 differentiation‐specific enhancer activity. We also identified a 60 kDa IPE binding (IPEB) protein whose activity is high in PYS‐2 cells, where IAP is expressed, but very low in F9 cells. Transcription of IAP elements has also been shown in the adult mouse thymus and in activated splenic B cells. We have now shown by DNA affinity chromatography, sodium dodecyl sulfate‐polyacrylamide gel electrophoresis, and band‐shift analysis that the 60 kDa IPEB is expressed in adult T lymphocytes and in resting as well as lipopolysaccharide activated splenic B cells but not in adult liver cells, suggesting an important role for IPEB in IAP transcription in vivo. In addition, we find IPEB expressed in the fetal mouse at sites of lymphoid development, such as the liver, spleen, and thymus, suggesting it may play an important role in gene expression during lymphoid development. In support of this, we find IPEB in the human T cell tumor lines, Jurkat and Molt 13, as well as the Daudi B cell line and in the normal calf thymus and in the thymus and spleen of the chicken and rat. © 1995 Wiley‐Liss, Inc.

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