Label-free detection of DNA using novel organic-based electrolyte-insulator-semiconductor

Tsung Wu Lin, Dhananjay Kekuda, Chih Wei Chu

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

In this study, we have constructed the first organic field effect sensor based on an electrolyte-insulator-semiconductor structure (OEIS) and applied this novel device to pH and DNA sensing. Variations in the insulator-electrolyte surface potential, which originate from either the change of the ionization states of the insulator surface groups or the binding of charged molecules to the insulator surface, modify the flat band voltage (VFB) of the OEIS sensor. The pH sensing experiments of OEIS sensor showed that the output signal linearly depended on pH solution in the range from pH 2 to pH 12, and an average sensitivity of 44.1mV/pH was obtained. In the biosensing experiments, the absorption of positively charged poly-l-lysine on the insulator surface resulted in the reduction of the VFB value, whereas the subsequent binding of negatively charged single-stranded DNA probe (ssDNA) via electrostatic interaction increased the VFB value. Furthermore, the ssDNA-immobilized OEIS device was successfully used for the detection of DNA hybridization. The detection limit of complementary DNA was as low as 1μM, and the output signal of OEIS biosensor linearly increased with the logarithm of complementary DNA concentration in the range from 5×10-5 to 10-7M. The easy and inexpensive fabrication of the OEIS device allows to be served as a potentially disposable and sensitive biosensor.

Original languageEnglish
Pages (from-to)2706-2710
Number of pages5
JournalBiosensors and Bioelectronics
Volume25
Issue number12
DOIs
Publication statusPublished - 01-08-2010

Fingerprint

Semiconductors
Electrolytes
Labels
DNA
Semiconductor materials
Semiconductor device structures
Single-Stranded DNA
DNA Probes
Biosensing Techniques
Biosensors
Equipment and Supplies
Sensors
Complementary DNA
Surface potential
Coulomb interactions
Static Electricity
Lysine
Ionization
Limit of Detection
Experiments

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Biophysics
  • Biomedical Engineering
  • Electrochemistry

Cite this

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abstract = "In this study, we have constructed the first organic field effect sensor based on an electrolyte-insulator-semiconductor structure (OEIS) and applied this novel device to pH and DNA sensing. Variations in the insulator-electrolyte surface potential, which originate from either the change of the ionization states of the insulator surface groups or the binding of charged molecules to the insulator surface, modify the flat band voltage (VFB) of the OEIS sensor. The pH sensing experiments of OEIS sensor showed that the output signal linearly depended on pH solution in the range from pH 2 to pH 12, and an average sensitivity of 44.1mV/pH was obtained. In the biosensing experiments, the absorption of positively charged poly-l-lysine on the insulator surface resulted in the reduction of the VFB value, whereas the subsequent binding of negatively charged single-stranded DNA probe (ssDNA) via electrostatic interaction increased the VFB value. Furthermore, the ssDNA-immobilized OEIS device was successfully used for the detection of DNA hybridization. The detection limit of complementary DNA was as low as 1μM, and the output signal of OEIS biosensor linearly increased with the logarithm of complementary DNA concentration in the range from 5×10-5 to 10-7M. The easy and inexpensive fabrication of the OEIS device allows to be served as a potentially disposable and sensitive biosensor.",
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Label-free detection of DNA using novel organic-based electrolyte-insulator-semiconductor. / Lin, Tsung Wu; Kekuda, Dhananjay; Chu, Chih Wei.

In: Biosensors and Bioelectronics, Vol. 25, No. 12, 01.08.2010, p. 2706-2710.

Research output: Contribution to journalArticle

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