Laboratory indicators of mastitis are not associated with elevated HIV-1 DNA loads or predictive of HIV-1 RNA loads in breast milk

Soren Gantt, Avinash K. Shetty, Kristy D. Seidel, Kuda Matasa, Georgina Musingwini, Godfrey Woelk, Lynn S. Zijenah, David A. Katzenstein, Lisa M. Frenkel

Research output: Contribution to journalArticlepeer-review

19 Citations (Scopus)

Abstract

Background. Mother-to-child transmission (MTCT) of HIV-1 has been associated with symptomatic and asymptomatic mastitis and with the quantity of HIV-1 RNA and DNA in maternal milk. An improved understanding of the relationship between indicators of inflammation and HIV-1 loads in breast milk could improve MTCT prevention strategies. Methods. In a cross-sectional study, laboratory indicators of mastitis (breast milk sodium [Na +] concentration, sodium:potassium ratio [Na +:K +], and leukocyte count) were related to breast milk HIV-1 RNA and DNA loads and were evaluated for predicting viral loads in milk. Results. Mastitis was present in 63 (15%) of 407, 60 (15%) of 407, and 76 (18%) of 412 milk specimens, as defined by Na + concentration >12 mmol/L, Na +:K + >1, and total leukocyte counts ≥106 cells/mL, respectively. Each indicator was associated with an increased milk HIV-1 RNA load (P < .05) but not with HIV-1 DNA load. Neutrophils correlated better with milk HIV-1 RNA load than total leukocytes. However, neither neutrophil count, Na + concentration, nor Na +:K + displayed a threshold that was both sensitive and specific for the detection of HIV-1 RNA in milk at thresholds of ≥50 or ≥10 4 copies/mL. Conclusions. HIV-1 DNA loads in breast milk were not increased during mastitis. Neither milk cell counts nor electrolyte concentrations were useful predictors of milk HIV-1 RNA or DNA loads for individual women.

Original languageEnglish
Pages (from-to)570-576
Number of pages7
JournalJournal of Infectious Diseases
Volume196
Issue number4
DOIs
Publication statusPublished - 15-08-2007
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Public Health, Environmental and Occupational Health
  • Immunology

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