Abstract
Sperm DNA damage affects sperm function, compromising reproductive outcome in both natural and assisted reproduction. The present study addresses the existing ambiguity between sperm morphology and DNA damage, by use of comet assay/single cell gel electrophoresis. Using comet assay as an end point, sperm DNA fragmentation in a total of 17 control and infertile subjects presenting to the infertility clinic of Kasturba Medical College has been investigated. Percent tail DNA, tail length and olive tail moment were selected as a measure to compare the extent of DNA damage between the groups. No significant difference was observed between the control group with morphologically normal sperm and experimental group with abnormal sperm morphology with respect to head DNA, tail DNA, Olive tail moment or tail length by alkaline comet assay. The comet assay failed to demonstrate any positive association between sperm morphology and DNA damage. In view of accumulating evidence on the importance of sperm DNA integrity in fertilization and embryogenesis, refined techniques allowing sperm selection with intact sperm DNA or with minimal DNA damage for clinical use should be sought.
| Original language | English |
|---|---|
| Pages (from-to) | 1345-1350 |
| Number of pages | 6 |
| Journal | Proceedings of the National Academy of Sciences India Section B - Biological Sciences |
| Volume | 88 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - 01-12-2018 |
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All Science Journal Classification (ASJC) codes
- Environmental Science(all)
- Agricultural and Biological Sciences(all)
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Lack of an Association Between Sperm Head Abnormality and DNA Damage by Alkaline Comet Assay. / Jayaraman, Varshini; Upadhya, Dinesh; Narayan, Pratap Kumar; Adiga, Satish Kumar.
In: Proceedings of the National Academy of Sciences India Section B - Biological Sciences, Vol. 88, No. 4, 01.12.2018, p. 1345-1350.Research output: Contribution to journal › Article
TY - JOUR
T1 - Lack of an Association Between Sperm Head Abnormality and DNA Damage by Alkaline Comet Assay
AU - Jayaraman, Varshini
AU - Upadhya, Dinesh
AU - Narayan, Pratap Kumar
AU - Adiga, Satish Kumar
PY - 2018/12/1
Y1 - 2018/12/1
N2 - Sperm DNA damage affects sperm function, compromising reproductive outcome in both natural and assisted reproduction. The present study addresses the existing ambiguity between sperm morphology and DNA damage, by use of comet assay/single cell gel electrophoresis. Using comet assay as an end point, sperm DNA fragmentation in a total of 17 control and infertile subjects presenting to the infertility clinic of Kasturba Medical College has been investigated. Percent tail DNA, tail length and olive tail moment were selected as a measure to compare the extent of DNA damage between the groups. No significant difference was observed between the control group with morphologically normal sperm and experimental group with abnormal sperm morphology with respect to head DNA, tail DNA, Olive tail moment or tail length by alkaline comet assay. The comet assay failed to demonstrate any positive association between sperm morphology and DNA damage. In view of accumulating evidence on the importance of sperm DNA integrity in fertilization and embryogenesis, refined techniques allowing sperm selection with intact sperm DNA or with minimal DNA damage for clinical use should be sought.
AB - Sperm DNA damage affects sperm function, compromising reproductive outcome in both natural and assisted reproduction. The present study addresses the existing ambiguity between sperm morphology and DNA damage, by use of comet assay/single cell gel electrophoresis. Using comet assay as an end point, sperm DNA fragmentation in a total of 17 control and infertile subjects presenting to the infertility clinic of Kasturba Medical College has been investigated. Percent tail DNA, tail length and olive tail moment were selected as a measure to compare the extent of DNA damage between the groups. No significant difference was observed between the control group with morphologically normal sperm and experimental group with abnormal sperm morphology with respect to head DNA, tail DNA, Olive tail moment or tail length by alkaline comet assay. The comet assay failed to demonstrate any positive association between sperm morphology and DNA damage. In view of accumulating evidence on the importance of sperm DNA integrity in fertilization and embryogenesis, refined techniques allowing sperm selection with intact sperm DNA or with minimal DNA damage for clinical use should be sought.
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U2 - 10.1007/s40011-017-0863-y
DO - 10.1007/s40011-017-0863-y
M3 - Article
VL - 88
SP - 1345
EP - 1350
JO - Proceedings of the National Academy of Sciences India Section B - Biological Sciences
JF - Proceedings of the National Academy of Sciences India Section B - Biological Sciences
SN - 0369-8211
IS - 4
ER -