Laser Raman tweezer spectroscopy to explore the bisphenol A-induced changes in human erythrocytes

Jijo Lukose, N. Mithun, M. Priyanka, Ganesh Mohan, Shamee Shastry, Santhosh Chidangil

Research output: Contribution to journalArticle

Abstract

The dermal penetration of bisphenol-A (BPA) from thermal papers into the human skin is a matter of major health concern due to its extensive use in developing countries like India, one of its largest users in the world. Bisphenol A is widely used in the manufacture of many consumer goods like polycarbonate water bottles, baby bottles, food containers, home appliances, thermal papers used in billing and tickets, the inner lining of food cans, etc. BPA can be easily adsorbed into the blood rapidly. The integration of optical tweezers with Raman spectroscopic techniques has realized avenues for interpreting single cell investigations. In the present work, the impact of BPA from thermal papers on individual human erythrocytes (red blood cells) has been investigated using micro-Raman spectroscopy. Significant intensity variations were noticed for hemoglobin oxygenation markers in the Raman spectra of red blood cells (RBCs). Raman spectral variations supporting RBC hemoglobin depletion were also found in the presence of BPA. Evident morphological changes are also observed in RBCs due to BPA in vitro exposures, which ultimately lead to cell bursting at higher concentrations.

Original languageEnglish
Pages (from-to)15933-15940
Number of pages8
JournalRSC Advances
Volume9
Issue number28
DOIs
Publication statusPublished - 01-01-2019

Fingerprint

Blood
Spectroscopy
Lasers
Hemoglobin
Bottles
polycarbonate
Cells
Hemoglobins
Optical tweezers
Domestic appliances
Oxygenation
Polycarbonates
Linings
Developing countries
Containers
Raman spectroscopy
Raman scattering
Skin
bisphenol A
Health

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Chemical Engineering(all)

Cite this

Lukose, Jijo ; Mithun, N. ; Priyanka, M. ; Mohan, Ganesh ; Shastry, Shamee ; Chidangil, Santhosh. / Laser Raman tweezer spectroscopy to explore the bisphenol A-induced changes in human erythrocytes. In: RSC Advances. 2019 ; Vol. 9, No. 28. pp. 15933-15940.
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Laser Raman tweezer spectroscopy to explore the bisphenol A-induced changes in human erythrocytes. / Lukose, Jijo; Mithun, N.; Priyanka, M.; Mohan, Ganesh; Shastry, Shamee; Chidangil, Santhosh.

In: RSC Advances, Vol. 9, No. 28, 01.01.2019, p. 15933-15940.

Research output: Contribution to journalArticle

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