LC method for the estimation of Cefprozil in human plasma and its pharmacokinetics

Shivprakash, S. Reddy, B. Dasandi, C. Patel, K. M. Bhat

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Abstract

A new method for the estimation of Cefprozil is developed and validated. This method is simple and rapid because the sample preparation is devoid of any solvent extraction. The plasma proteins were precipitated and the supernatant was directly injected to the HPLC system. The separation was achieved using RP 18e (5μ, 250 X 4 mm) column and the mobile phase was a mixture of Phosphate buffer and acetonitrile. The eluents monitored at 235nm. The column was maintained at a temperature of 40±0.1°C. The method was validated for sensitivity, specificity, precision and accuracy. The LOQ of this method is 0.25μg ml1 and the calibration curve range is 0.25 to 30μg ml-1. This method is used to study the comparative bioavailability of conventional and sustained release capsules of Cefprozil.

Original languageEnglish
Pages (from-to)86-90
Number of pages5
JournalIndian Drugs
Volume41
Issue number2
Publication statusPublished - 01-02-2004

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All Science Journal Classification (ASJC) codes

  • Pharmacology
  • Pharmaceutical Science
  • Drug Discovery

Cite this

Shivprakash, Reddy, S., Dasandi, B., Patel, C., & Bhat, K. M. (2004). LC method for the estimation of Cefprozil in human plasma and its pharmacokinetics. Indian Drugs, 41(2), 86-90.