Light emitting diode (LED) based fluorescence microscopy for tuberculosis detection: a review

Anushka Ojha, Soumyabrata Banik, Sindhoora Kaniyala Melanthota, Nirmal Mazumder

Research output: Contribution to journalArticle

Abstract

Since time immemorial, tuberculosis (TB) has intimidated the human race owing to its severity. Its socio-economic burden has led to it being a major cause of concern. It is one of the world’s major causes of death from a single agent. Since most of the middle- and low-income countries are burdened with TB, sputum smear examination using conventional light microscopy is often the only resort for diagnosing TB. However, fluorescence microscopy is used as standard in most high-income countries, owing to its increased sensitivity. Light-emitting diodes (LEDs), being inexpensive, are increasingly gaining popularity as an alternative light source for fluorescence microscopy. It has been found to be highly efficient and has a lot of advantages over the conventional Ziehl-Neelsen-based bright field microscopy. In this review, we discuss about the usefulness of LED-based fluorescence microscopy in diagnosing TB and how it is superior to the other sources of light used.

Original languageEnglish
JournalLasers in Medical Science
DOIs
Publication statusAccepted/In press - 01-01-2020

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Fluorescence Microscopy
Tuberculosis
Light
Microscopy
Sputum
Cause of Death
Economics

All Science Journal Classification (ASJC) codes

  • Surgery
  • Dermatology

Cite this

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abstract = "Since time immemorial, tuberculosis (TB) has intimidated the human race owing to its severity. Its socio-economic burden has led to it being a major cause of concern. It is one of the world’s major causes of death from a single agent. Since most of the middle- and low-income countries are burdened with TB, sputum smear examination using conventional light microscopy is often the only resort for diagnosing TB. However, fluorescence microscopy is used as standard in most high-income countries, owing to its increased sensitivity. Light-emitting diodes (LEDs), being inexpensive, are increasingly gaining popularity as an alternative light source for fluorescence microscopy. It has been found to be highly efficient and has a lot of advantages over the conventional Ziehl-Neelsen-based bright field microscopy. In this review, we discuss about the usefulness of LED-based fluorescence microscopy in diagnosing TB and how it is superior to the other sources of light used.",
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Light emitting diode (LED) based fluorescence microscopy for tuberculosis detection : a review. / Ojha, Anushka; Banik, Soumyabrata; Melanthota, Sindhoora Kaniyala; Mazumder, Nirmal.

In: Lasers in Medical Science, 01.01.2020.

Research output: Contribution to journalArticle

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