Long-term exposure to a butter-rich diet induces mild-to-moderate steatosis in Chang liver cells and Swiss albino mice models

Thomas John Philip Nalloor, Nitesh Kumar, Kasinathan Narayanan, Vasanth Raj Palanimuthu

Research output: Contribution to journalArticle

Abstract

Butter is one of the widely used fats present in the diet. However, there is no satisfactory study available that evaluates the effect of a high-fat diet containing butter as the principal fat on the development of non-alcoholic fatty liver disease (NAFLD). In the present study, butter was used for the development of steatosis in Chang liver cells in an in vitro study and Swiss albino mice in an in vivo study. In vitro steatosis was established, and butter was compared with oleic acid in Chang liver cells using an oil red O (ORO)-based colorimetric assay. In the in vivo study, a butter-rich special diet was fed for 15 weeks to mice, who showed no significant change in body weight. The expression pattern of phosphatase and tensin homolog (PTEN) and miR-21 was compared by reverse transcriptase-PCR. Special diet-fed animals showed downregulated PTEN compared to normal diet-fed animals, while levels of miR-21 remained the same. Elevations in biochemical parameters, viz., triglycerides and liver function tests showed symptoms of onset of NAFLD. Histophathological study of livers of test animals confirmed mild-to-moderate degree of NAFLD.

Original languageEnglish
Pages (from-to)257-265
Number of pages9
JournalJournal of Basic and Clinical Physiology and Pharmacology
Volume28
Issue number3
DOIs
Publication statusPublished - 01-05-2017

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Butter
Diet
Liver
Phosphoric Monoester Hydrolases
Fats
Body Weight Changes
Liver Function Tests
High Fat Diet
Oleic Acid
Reverse Transcriptase Polymerase Chain Reaction
Triglycerides
Down-Regulation
Non-alcoholic Fatty Liver Disease

All Science Journal Classification (ASJC) codes

  • Physiology
  • Pharmacology
  • Drug Discovery

Cite this

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Long-term exposure to a butter-rich diet induces mild-to-moderate steatosis in Chang liver cells and Swiss albino mice models. / Nalloor, Thomas John Philip; Kumar, Nitesh; Narayanan, Kasinathan; Palanimuthu, Vasanth Raj.

In: Journal of Basic and Clinical Physiology and Pharmacology, Vol. 28, No. 3, 01.05.2017, p. 257-265.

Research output: Contribution to journalArticle

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