Method development and validation for the estimation of Ticargrelor in bulk and comparison with other published methods

Nazare Lobo John Shane, Ashish Hanumantrao Chamle, S. G. Vasantharaju, Aravind Pai, B. S. Muddukrishna

Research output: Contribution to journalArticle

Abstract

The following article consist of highly sensitive and easy RP-HPLC method to estimate Ticagrelor in bulk. Ticagrelor was separated using Shimadzu LC-20-AD with auto sampler and PDA/UV detector. The separation was achieved using a C18 Vydac Monomeric 120A (5.0 micron, 250 x 4.6mm) column. Acetonitrile: Water Milli Q (60:40 v/v) at a flow rate of 1.0 ml/min was used as the mobile phase. The method was linear having line equation f(x)=1.90442e-005*x-0.302059 with correlation coefficient (R2) of 0.997. The method was precise having RSD as 0.27% (intraday precision). The LOD & LOQ were 0.083μg/mL and 0.25μg/mL respectively. The developed method was validated for precision, linearity, system suitability and robustness; hence proves that it is highly applicable to estimate Ticagrelor in day to day analysis.

Original languageEnglish
Pages (from-to)1-6
Number of pages6
JournalJournal of Global Pharma Technology
Volume8
Issue number12
Publication statusPublished - 2016

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High Pressure Liquid Chromatography
Water
Ticagrelor
AD 20
acetonitrile

All Science Journal Classification (ASJC) codes

  • Pharmaceutical Science

Cite this

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title = "Method development and validation for the estimation of Ticargrelor in bulk and comparison with other published methods",
abstract = "The following article consist of highly sensitive and easy RP-HPLC method to estimate Ticagrelor in bulk. Ticagrelor was separated using Shimadzu LC-20-AD with auto sampler and PDA/UV detector. The separation was achieved using a C18 Vydac Monomeric 120A (5.0 micron, 250 x 4.6mm) column. Acetonitrile: Water Milli Q (60:40 v/v) at a flow rate of 1.0 ml/min was used as the mobile phase. The method was linear having line equation f(x)=1.90442e-005*x-0.302059 with correlation coefficient (R2) of 0.997. The method was precise having RSD as 0.27{\%} (intraday precision). The LOD & LOQ were 0.083μg/mL and 0.25μg/mL respectively. The developed method was validated for precision, linearity, system suitability and robustness; hence proves that it is highly applicable to estimate Ticagrelor in day to day analysis.",
author = "Shane, {Nazare Lobo John} and Chamle, {Ashish Hanumantrao} and Vasantharaju, {S. G.} and Aravind Pai and Muddukrishna, {B. S.}",
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Method development and validation for the estimation of Ticargrelor in bulk and comparison with other published methods. / Shane, Nazare Lobo John; Chamle, Ashish Hanumantrao; Vasantharaju, S. G.; Pai, Aravind; Muddukrishna, B. S.

In: Journal of Global Pharma Technology, Vol. 8, No. 12, 2016, p. 1-6.

Research output: Contribution to journalArticle

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AU - Shane, Nazare Lobo John

AU - Chamle, Ashish Hanumantrao

AU - Vasantharaju, S. G.

AU - Pai, Aravind

AU - Muddukrishna, B. S.

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