Method development and validation for the estimation of Ticargrelor in bulk and comparison with other published methods

Nazare Lobo John Shane, Ashish Hanumantrao Chamle, S. G. Vasantharaju, Aravind Pai, B. S. Muddukrishna

Research output: Contribution to journalArticle


The following article consist of highly sensitive and easy RP-HPLC method to estimate Ticagrelor in bulk. Ticagrelor was separated using Shimadzu LC-20-AD with auto sampler and PDA/UV detector. The separation was achieved using a C18 Vydac Monomeric 120A (5.0 micron, 250 x 4.6mm) column. Acetonitrile: Water Milli Q (60:40 v/v) at a flow rate of 1.0 ml/min was used as the mobile phase. The method was linear having line equation f(x)=1.90442e-005*x-0.302059 with correlation coefficient (R2) of 0.997. The method was precise having RSD as 0.27% (intraday precision). The LOD & LOQ were 0.083μg/mL and 0.25μg/mL respectively. The developed method was validated for precision, linearity, system suitability and robustness; hence proves that it is highly applicable to estimate Ticagrelor in day to day analysis.

Original languageEnglish
Pages (from-to)1-6
Number of pages6
JournalJournal of Global Pharma Technology
Issue number12
Publication statusPublished - 2016


All Science Journal Classification (ASJC) codes

  • Pharmaceutical Science

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